非洲猪瘟病毒基因Ⅰ型的双重实时荧光定量PCR检测方法建立

doi:10.11843/j.issn.0366-6964.2023.10.039

Acta Veterinaria et Zootechnica Sinica ›› 2023, Vol. 54 ›› Issue (10): 4428-4432.doi: 10.11843/j.issn.0366-6964.2023.10.039

• RESEARCH NOTES • Previous Articles Next Articles

Establishment of a Dual Real-time Fluorescence Quantitative PCR Assay for Genotype I ASFV

CHANG Hao^1,2,3, QIU Yingwu^1,2,3, PENG Jie^1,2,3, GAO Qi^1,3, SONG Zebu^1,3,4, CHEN Yang^1,3,5, LI Wei², LIN Limiao², CAO Xuezhen², ZHOU Qingfeng², ZHANG Guihong^1,3,4, LI Qunhui^2*, ZHENG Zezhong^1,3,4*

1. African Swine Fever Prevention and Control Technology Research Center, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
2. Guangdong Provincial Key Laboratory of Livestock and Poultry Health Breeding and Environmental Control, Wens Food Group Co., Ltd., Yunfu 527400, China;
3. National African Swine Fever Regional Laboratory (Guangzhou), Guangzhou 510642, China;
4. Guangdong Provincial Key Laboratory of Prevention and Control of Zoonotic Diseases of Animal Origin, Guangzhou 510642, China;
5. Maoming Branch of Guangdong Laboratory of Lingnan Modern Agricultural Science and Technology, Maoming 525000, China

Received:2022-11-14 Online:2023-10-23 Published:2023-10-26

Abstract

Abstract: With the beginning of Genotype I African swine fever (Genotype I ASFV) epidemic in China, it makes the diagnosis, prevention and control of ASF in China more challenging. In this study, primers and probes of B646L recommended by China Animal Disease Control Center (CADC) was used, combined with primers and probes designed for the conserved specific region of GenotypeⅠ ASFV EP402R gene, to establish a rapid detection method that can identify GenotypeⅠ ASFV while diagnosing ASF, and its sensitivity and specificity were tested. The results show that the method has good sensitivity and specificity and can be effectively used for the clinical diagnosis of ASFV and the monitoring of GenotypeⅠ ASFV.

Key words: African swine fever, genotype I ASFV, real-time fluorescence quantitative PCR, EP402R, B646L

CLC Number:

S852.651

CHANG Hao, QIU Yingwu, PENG Jie, GAO Qi, SONG Zebu, CHEN Yang, LI Wei, LIN Limiao, CAO Xuezhen, ZHOU Qingfeng, ZHANG Guihong, LI Qunhui, ZHENG Zezhong. Establishment of a Dual Real-time Fluorescence Quantitative PCR Assay for Genotype I ASFV[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4428-4432.

References 8

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Establishment of a Dual Real-time Fluorescence Quantitative PCR Assay for Genotype I ASFV

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