不同类型肉鸡线粒体单倍型与遗传起源研究

doi:10.11843/j.issn.0366-6964.2022.11.004

Abstract

Abstract: The aim of this study was to investigate the haplotype characteristics of the mitochondrial D-loop region in chickens and its correlation with growth rate, and to analyze its genetic origin. Six different types of broiler breeds (synthetic lines) were selected to determine their production performance. The full length of the mitochondrial D-loop region of 314 individuals from the 6 groups were sequenced to analyze their haplotype characteristics, and clustered with different jungle fowl subspecies to analyze their maternal origin. The results showed that a total of 37 variant loci were detected in the whole sequences of D-loop regions of the 6 populations, constituting 40 haplotypes, and were divided into 4 haplotype groups A, B, C and E. Among them, AA broilers, Ross 308, Qinyan partridge chicken and Yuhe No.1 broilers were all mainly E haplotypes, accounting for 85.92%, 50.00%, 100.00% and 70.21%, respectively. Yuanfeng partridge chicken No.2 was mainly B haplotype (66.67%) and Gangfeng yao black partridge chicken was mainly C haplotypes (40.30%). While the proportions of E haplotype in the two chicken populations were relatively low, with 15.00% and 22.39%, respectively. Correlation analysis showed that birth weight was positively correlated with the proportion of haplotype E (P<0.01), and negatively correlated with the proportion of haplotype A, B and C. There was a significantly negative correlation between the ratio of E haplotype and the age of the average body weight of males and females at about 1.8 kg (P<0.05). And there was a very significantly negative correlation between the ratio of E haplotype and the feed conversion ratio of average body weight of males and females at about 1.8 kg (P<0.01). Cluster analysis showed that all individuals with A and B haplotypes clustered with gallus gallus spadiceus, all individuals with E haplotypes clustered with gallus gallus murgha, all individuals with C haplotypes clustered with gallus gallus murghi, gallus gallus spadiceus, gallus gallus gallus and gallus gallus bankiva. The results indicated that there is a significant correlation between mitochondrial haplotype and the growth rate of broilers, E haplotype had a strong positive correlation with broiler growth rate. The local chicken breeds in China had multiple maternal origins, mainly originated from Gallus gallus spadiceus.

Key words: chicken, mitochondrial haplotype, production performance, genetic origins

CLC Number:

S831.2

TANG Xiujun, FAN Yanfeng, JIA Xiaoxu, GE Qinglian, LU Junxian, ZHOU Qian, CHEN Dawei, GAO Yushi. Studies on Mitochondrial Haplotype and Genetic Origins of Different Types Broilers[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3748-3758.

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Studies on Mitochondrial Haplotype and Genetic Origins of Different Types Broilers

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