XRCC1在卵子和早期胚胎中的定位与功能的初步分析

doi:10.11843/j.issn.0366-6964.2023.05.033

Abstract

Abstract: XRCC1 is a molecular scaffold protein that plays a central role in the base excision repair pathway. Here, we examined the subcellular localization of XRCC1 and investigated its effects on meiotic maturation of oocytes, subsequent embryonic development, and global DNA methylation in mice. XRCC1 was found to be localized mainly in the nuclei during oocyte maturation and subsequent embryonic development. Blocking XRCC1 by microinjection with antibodies partly reduced oocyte germinal vesicle breakdown (GVBD) and embryo cleavage, and also disrupts DNA demethylation in the 2-cell embryos. The results indicates that XRCC1 is mainly located in the nuclei at the interphase of cell cycle, and XRCC1 with normal function contributes to the maintenance of the quality of mouse oocytes during meiosis and the normal development of early embryos.

Key words: XRCC1, mouse embryo, DNA demethylation, oocyte

CLC Number:

S852.1

ZHU Jiaqiao, CHENG Laiyang, CAO Jiangqin, ZHU Min, LI Junwei, JU Huimin, LIU Zongping. Preliminary Study on the Location and Function of XRCC1 in Oocyte and Early Embryos[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2126-2133.

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Preliminary Study on the Location and Function of XRCC1 in Oocyte and Early Embryos

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