卵母细胞成熟相关激素和生长因子受体在马扩展型和紧凑型卵丘-卵母细胞复合体表达的研究

doi:10.11843/j.issn.0366-6964.2023.09.014

Abstract

Abstract: The present study was conducted to compare the expression of some growth factors and hormone receptors associated with oocyte maturation in Ex-COC and Cp-COC to investigate the reasons for the difference in maturation and development capacity in the two types of equine oocytes. Isolated ovaries from Mongolian horses were collected at the slaughterhouse and brought back to the laboratory to recover COCs, Ex- and Cp-COCs were distinguished according to cumulus morphology; the expression pattern of FSHR, LHR, IGF1R, IGF2R, ESR1, ESR2, the BMP15 and GDF9 receptors BMPR1B, BMPR2, and ALK5 were studied by qPCR and immunofluorescence in both COCs. Estradiol, IGF2, GDF9, and BMP15 were added to the equine IVM culture system to examine their effects on the maturation rate of equine oocytes in vitro. The results showed that the expression of these receptor genes was difference in cumulus cells and oocytes between Ex- and Cp-COCs, with most receptor genes being expressed at higher levels in cumulus than in oocytes in Cp-COCs, while in Ex-COCs, the levels in cumulus were similar with oocytes or higher in oocytes. Most of the receptor genes showed higher expression levels in oocytes from Ex-COCs compared to oocytes from Cp-COCs. In vitro maturation culture experiments revel that the addition of estrogen and IGF2 may be beneficial in increasing the rate of maturation of equine oocytes in vitro. Different expression levels of the important hormones or growth factor receptors such as FSH, LH, IGF1, IGF2, estrogen, BMP15, and GDF9 in equine Ex- and Cp-COCs could explain the differences in maturation and developmental capacity in vitro, and the addition of IGF2 and E2 to the maturation culture system may increase the in vitro maturation rate of equine oocytes.

Key words: equine oocyte, FSH, IGF1/2, estrogen, GDF9, BMP15, IVM

CLC Number:

S821.2

SHEN Yingchao, DAVSHILT Toli, REN Hong, WANG Xisheng, TIAN Shuyue, DU Ming, DUGARJAVIIN Manglai, BOU Gerelchimeg. Differential Expression of Oocyte Development-related Hormone and Growth Factor Receptors in Equine Expanded and Compact Cumulus-oocyte Complexes[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3735-3744.

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Differential Expression of Oocyte Development-related Hormone and Growth Factor Receptors in Equine Expanded and Compact Cumulus-oocyte Complexes

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