PLCγ1基因对绵羊早期胚胎体外发育的影响

doi:10.11843/j.issn.0366-6964.2022.08.016

Abstract

Abstract: The purpose of this study was to investigate the effect of PLCγ1 on the development of sheep early embryo in vitro, and to lay a foundation for revealing the action mechanism of PLCγ1 in the development of sheep early embryo. In this study, sheep oocytes with more than 3 layers of granulosa cells were selected as experimental materials, and they were divided into two groups after mature in vitro culture. PLCγ1 was introduced into MⅡ oocytes (n=127) by microinjection as experimental group. Microinjection of empty vector pcDNA3.1-EGFP was used as the control group (n=120), and after 48 h the cleavage rate were measured. The Ca²⁺ probe Rhod 2-AM was used to monitor the Ca²⁺ fluctuation of MⅡ oocytes and early embryos at 16, 48, 72, 96 and 120 h after microinjection. The results showed that the cleavage rate was ((19.67±0.2)%, P<0.01)) and morula development rate was ((9.00±0.17)%,P<0.05)) after microinjection of PLCγ1. After injection of PLCγ1, Ca²⁺ concentration in oocytes and early embryos varied at different developmental stages.It was observed that Ca²⁺ was mainly distributed in the cytoplasm and reached the peak at 48 h after injection (P<0.01). The results showed that PLCγ1 gene could induce Ca²⁺ oscillation during ovine oocyte development, initiate cleavage, and promote the development of early embryo.

Key words: PLCγ1, Ca²⁺, oocyte, morula, sheep

CLC Number:

S826.3

WU Xiaoxue, YUAN Liming, LIU Xinjie, LIU Suping, CHEN Ning, SAIWU Jiafu. Effect of PLCγ1 Gene on the Development of Sheep Early Embryo in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2578-2585.

References 35

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Effect of PLCγ1 Gene on the Development of Sheep Early Embryo in vitro

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