猪丹毒丝菌灭活疫苗免疫佐剂的筛选

doi:10.11843/j.issn.0366-6964.2020.05.019

Abstract

Abstract: The objective of this study was to screen the adjuvant suitable for an inactivated vaccine against Erysipelothrix rhusiopathiae. The five inactivated vaccines of mineral oil adjuvant (referred as oil vaccine), aluminum hydroxide adjuvant (referred as alh vaccine), ISA201 dual phase oil emulsion adjuvant (referred as ISA201vaccine), GEL02 water-soluble polymer adjuvant (referred as GEL vaccine), and IMS1313 water-soluble nano-sized adjuvant (referred as IMS1313 vaccine) were prepared, which the antigens of that were all inactivated bacteria of E. rhusiopathiae strain HG-1, isolated and identified as serovar 1a. The immunoprotection test results of the mouse showed that after 14 days of second immunization, mice were intraperitoneally challenged with 4LD₅₀ HG-1 solution, giving the protection rates of the five vaccines 100% (7/7), 14% (1/7), 14% (1/7), 71% (5/7) and 14% (1/7), respectively. According to the above test results, the safety test of piglets was carried out by using alh vaccine and GEL vaccine, and the results showed that the side effects of the two adjuvant vaccines were small. Fourteen days after the second immunization, groups of pigs vaccinated with alh vaccine and GEL vaccine were challenged intravenously with 16 LD₁₀₀ of HG-1 solution, and the results showed that alh vaccine and GEL vaccine provide 60% (3/5) and 100% (5/5) protective efficacy, respectively. This study identified GEL adjuvant as the optimal adjuvant for the development of an inactivated vaccine against swine erysipelas.

Key words: swine, Erysipelothrix rhusiopathiae, vaccine, adjuvant

CLC Number:

S858.285.1

PEI Shixuan, SONG Jijian, HAN Yinong, XUE Yun, WANG Chen, SI Lifang, ZHAO Zhanqin. Comparison of Adjuvant for Inactivated Swine Erysipelas Vaccines against Erysipelothrix rhusiopathiae[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1083-1090.

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Comparison of Adjuvant for Inactivated Swine Erysipelas Vaccines against Erysipelothrix rhusiopathiae

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