高效赤红球菌协同CpG ODN增强鸡禽流感疫苗的免疫效力

doi:10.11843/j.issn.0366-6964.2022.12.022

Abstract

Abstract: Avian vaccines have problems of low immunization efficiency, short immunization duration and insufficient cellular immune stimulation. New avian vaccine adjuvants need to be developed and used to solve these problems. As reported in our former studies, CpG ODN has high safety and good cellular and humoral immune stimulation functions. However, the expensive cost of CpG ODN prevents its promotion in avian vaccines. In order to reduce cost and improve efficacy, based on the potential synergistic relationship between TLR2 and TLR21, this study investigated the relationship between Rhodococcus ruber and CpG ODN to synergistically stimulate immunity by utilizing the avian influenza vaccine as a model vaccine. Through the HI test, cell proliferation test and detection of the expression of related cytokines, we confirmed that the compound adjuvant which induced Th1 immunity has stronger humoral immunity and cellular immunity, a long-last time protection, as well as systemic and local immunity enhancement. By examining the relative expression levels of the receptors, we confirmed that there is a synergistic relationship between CpG ODN and Rhodococcus ruber. In addition, in the compound adjuvant stimulation experiment of halving the dose of CpG ODN, we found that due to the existence of synergy, the compound adjuvant with half dose CpG ODN still had good immune stimulation effect. This study made a preliminary exploration for further reducing the production and use costs of CpG preparations, and laid a foundation for the efficient and cost-controllable promotion of CpG preparations in poultry animal breeding and even animal husbandry.

Key words: effective adjuvant, CpG ODN, Rhodococcus ruber, Th1 immunity

CLC Number:

PAN Weixiong, CHEN Yuyi, ZHAO Zengjue, FENG Saixiang, QIU Weihong, YE Hejia, ZHANG Linghua. Rhodococcus ruber Synergizes Effectively with CpG ODN to Enhance the Immune Efficacy of the Bird Flu Vaccine in Chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4367-4378.

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Rhodococcus ruber Synergizes Effectively with CpG ODN to Enhance the Immune Efficacy of the Bird Flu Vaccine in Chickens

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