苯基吡啶酮衍生物影响猪δ冠状病毒复制的作用分析

doi:10.11843/j.issn.0366-6964.2022.09.033

Abstract

Abstract: The purpose of this study was to investigate the antiviral effect of phenylpyridinone derivative JIB-04 on porcine deltacoronavirus (PDCoV), and explore the possible mechanism. Cell viability after treatment with different concentrations of JIB-04 was detected by CCK-8, and the 50% cytotoxic concentration (CC₅₀) and 50% effective concentration (EC₅₀) were calculated. TCID₅₀ method was used to detect the effects of JIB-04 pretreatment and co-treatment on PDCoV replicationand the effect of JIB-04 treatment on virus attachment and penetration. Finally, qRT-PCR, TCID₅₀ and Western blot methods were used to detect the effect of JIB-04 on virus replication at different times post infection. The results showed that JIB-04 did not affect the cell viability of LLC-PK cells at all tested concentrations, and CC₅₀>640 μmol·L^-1, EC₅₀=0.216 μmol·L^-1, and SI index is greater than 2 963. Compared with untreated virus infection group, JIB-04 treatment significantly reduced the virus titer (P<0.001), but it had no effect on attachment or penetration of PDCoV. At 6 h post infection, compared with untreated virus infection group, virus titer in JIB-04 treatment group was significantly decreased (P<0.01). At 12 and 24 h post infection, virus titer, genome copy number, and N protein expression level all significantly decreased (P<0.01). JIB-04 has a low cytotoxicity and a high selective index, and can protect against PDCoV infection in vitro, making it a potential antiviral drug. JIB-04 can inhibit synthesis of viral RNA, protein and PDCoV replication.

Key words: phenylpyridinone derivative JIB-04, antiviral, porcine deltacoronavirus

CLC Number:

R978.7

YAO Chen, GUO Meng, HU Hui, SHI Chenxi, YANG Guoyu. Effects of Phenylpyridinone Derivative on the Replication of Porcine Deltacoronavirus[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3190-3198.

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Effects of Phenylpyridinone Derivative on the Replication of Porcine Deltacoronavirus

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