牛坏死杆菌43K OMP基因缺失株生物学特性分析

doi:10.11843/j.issn.0366-6964.2022.11.027

Abstract

Abstract: The aim of this study was to investigate the biological function of the 43K OMP gene of Fusobacterium necrophorum. In vitro growth rate, drug sensitivity, biofilm formation ability, bacterial adhesion ability, bacterial toxicity to cells and pathogenicity biological properties to Balb/c female mice between the wild type strain (A25 strain) and 43K OMP gene defective strain (A25Δ43K OMP strain) were detected to investigate the potential biological function of 43K OMP gene in the pathogenesis of Fusobacterium necrophorum. The results showed that when 43K OMP gene was mutated, there was no significant difference in growth rate or bacterial toxicity to cells of F. necrophorum (P>0.05); Resistance to claricid, kanamycin, neomycin, amikacin, netilmicin, furantoin, polymyxin B, rifampicin and ciprofloxacin were diminished; Biofilm formation ability was significantly enhanced after 72 and 96 h of incubation (P<0.05); After co-incubation with BEND cells for 1 h, the bacterial adhesion ability was significantly reduced (P<0.05); The pathogenicity to Balb/c female mice was significantly weakened (P<0.05). Therefore, 43K OMP gene is mainly related to the bacterial adhesion ability, biofilm formation ability and drug resistance of F. necrophorum.

Key words: Fusobacterium necrophorum, 43K OMP, cell adhesion, biofilm, drug resistance

CLC Number:

S852.615

JIANG Kai, ZHAO Pengyu, WANG Tianshuo, YU Siwen, BI Lan, XIAO Jiawei, HE Xianjing, GUO Donghua. Analysis of Biological Characteristics of Fusobacterium necrophorum 43K OMP Genes Mutant Strain[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4019-4026.

References 27

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Analysis of Biological Characteristics of Fusobacterium necrophorum 43K OMP Genes Mutant Strain

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