C型产气荚膜梭菌外毒素致小鼠肠道损伤的转录组分析

doi:10.11843/j.issn.0366-6964.2022.10.029

Abstract

Abstract: The present study aimed to explore the toxicity mechanism of inflammatory injury and immune regulation disorders in organisms, and lay a foundation for further in-depth exploration of the pathogenic mechanism of Clostridium perfringens disease. The cultured supernatant of C. perfringens type C was intraperitoneally injected into BALB/c mice, and the small intestine samples were collected for transcriptome sequencing. Based on the differentially expressed genes (DEGs), GO functional annotation and KEGG pathway enrichment analysis were performed. The results showed that in total, there were 40.99 Gb effective bases and 795 DEGs were obtained, among which 229 were up-regulated and 566 were down-regulated. The ten randomly selected DEGs were verified by real-time fluorescent quantitative PCR that their relative expression was consistent with the transcriptional expression profile. GO functional annotation mainly involved G-protein coupled nucleotide receptor activity, and G-protein coupled purine nucleotide receptor activity. KEGG pathway enrichment analysis found that it was mainly concentrated in TNF signaling pathway, IL-17 signaling pathway, p53 signaling pathway, FOXO signaling pathway, Toll-like receptor signaling pathway, and NF-κB signaling pathway. When C. perfringens exotoxin invades the body, inflammatory signaling pathways such as TNF will be activated, resulting in inflammatory damage and even necrosis of the intestine.

Key words: Clostridium perfringens, transcriptome, intestine, inflammatory signaling pathway

CLC Number:

ZHANG Siyu, WANG Yujiong, ZENG Jin. Transcriptome Analysis of Intestinal Injury Induced by Clostridium perfringens Type C Exotoxin in Mouse[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3570-3581.

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Transcriptome Analysis of Intestinal Injury Induced by Clostridium perfringens Type C Exotoxin in Mouse

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