黄牛源产气荚膜梭菌分离株基因组的生物信息学分析

doi:10.11843/j.issn.0366-6964.2024.04.032

Abstract

Abstract: In this study, two strains of Clostridium perfringens type A, named ZWCP209 and ZWCP210, were isolated from the tissues and organs of sudden death Hainan cattle. Genome assembly results showed that the genome size was between 3.4-3.5 Mb, and the number of tRNA and CDS was stable. Multilocus sequence analysis (MLST) showed that the sequenced strains belonged to the same new ST type. The genomes of 27 bovine C. perfringens strains were obtained from the NCBI database and analyzed by bioinformatics together with sequenced isolates: A total of 13 resistance genes were detected, among which the tetracycline resistance genes tetA(P) and tetB(P) had the highest carrying rate (79.4%). It is noteworthy that the two Hainan cattle isolates in this study carried more than 7 resistance genes, including optrA, which is resistant to oxazolidinone for non-veterinary use. Pan-genome analysis showed that the genomes of these strains contained a total of 7 345 genes, and the number of core genes accounted for 22.94%. In the phylogenetic analysis of the core genome and plc gene, the two Hainan cattle isolates were in the same branch and had the same virulence factors, with high homology. This study is the first complete genome sequencing and bioinformatics analysis of C. perfringens isolated from cattle in Hainan Province in China, which has a reference value for the prevention and treatment of C. perfringens disease in cattle and the study of genome.

Key words: Hainan cattle, Clostridium perfringens, isolation and identification, Whole genome sequencing, bioinformatics analysis

CLC Number:

S852.616.3

TIAN Rui, XU Sixiang, XIE Feng, LIU Guangjin, WANG Gang, LI Qingxia, DAI Lei, XIE Guoxin, ZHANG Qiongwen, LU Yajing, WANG Guangwen, WANG Jinxiu, ZHANG Wei. Bioinformatics Analysis of the Genome of Clostridium perfringens Isolated from Cattle[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1707-1715.

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Bioinformatics Analysis of the Genome of Clostridium perfringens Isolated from Cattle

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