甘草查尔酮A与三种抗生素联用对产气荚膜梭菌感染小鼠的治疗作用

doi:10.11843/j.issn.0366-6964.2024.01.031

Abstract

Abstract: The aim of this study was to investigate the effect of Chinese herbal monomers in combination with antibiotics on Clostridium perfringens (CP) infection in mice. In this study, licorice chalcone A (LCA) was used as a representative drug to investigate the in vitro and in vivo therapeutic effects of LCA in combination with antibiotics. Some antibiotics were identified by combined drug sensitivity tests as having synergistic effects with the monomeric LCA of Chinese medicine. The therapeutic effect of the three antibiotics in combination with LCA at different concentrations was compared by measuring the bactericidal profile of Clostridium perfringens at different concentrations, the hemolysis test, the sliding test and therapeutic effect on gas gangrene in mice. Three antibiotics, clindamycin (CLDM), tetracycline (TCN), and tilmicosin (TMS), which have synergistic effects with the LCA, were identified by combined drug sensitivity tests for follow-up trials. According to the bactericidal curve results, LCA at 8 μg·mL^-1 and TMS at 16 μg·mL^-1 can almost completely kill Clostridium perfringens. The combination of LCA and TMS (both 2 μg·mL^-1) significantly reduced bacterial hemolysis and hemolysis was almost completely inhibited (hemolysis quantified at 9.08%). It is worth noting that LCA has a facilitating effect on bacterial mobility and can improve hair-mediated motility, and its facilitating effect is significantly enhanced when combined with TMS. The results of the animal studies showed that CLDM alone had a good inhibitory effect on Clostridium perfringens and was clinically effective; TCN and TMS each showed a synergistic effect (FIC index of 0.375) when combined with LCA, and also showed a good synergistic effect in treatment. In practice, therefore, TMS can be used in combination with LCA in the treatment of Clostridium perfringens infections to reduce the amount of medication used while improving the therapeutic effect.

Key words: licorice chalcone A, Clostridium perfringens, gas gangrene, tilmicosin, tetracycline, clindamycin

CLC Number:

S859.7

ZHOU Wenhui, BAO Hongxia, WANG Junhao, HUANG Yuanling, WANG Wenhui, HAO Haihong. Therapeutic Effect of Licorice Chalcone A in Combination with Three Antibiotics on Clostridium perfringens Infection in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 334-345.

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Therapeutic Effect of Licorice Chalcone A in Combination with Three Antibiotics on Clostridium perfringens Infection in Mice

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