1株鸡滑液囊支原体的分离鉴定与全基因组分析

doi:10.11843/j.issn.0366-6964.2022.08.024

Abstract

Abstract: Mycoplasma synoviae (MS) can cause respiratory diseases, infectious synovitis, abnormal eggshell top and growth retardation in poultry, and bring huge economic losses to the global poultry industry. However, there are differences in drug resistance and pathogenicity of MS in different countries and regions. Therefore, this study aims to explore the characteristics of MS strains in Fujian. The contents of the articular cavity of chickens suspected of suffering from MS in Fujian were collected, the strains were isolated and identified, and the whole genome was sequenced by third-generation combined with second-generation sequencing technology. The results showed that the pathogenic characteristics of the isolate were stable and had been deposited in China center for type culture collection. The preservation number was CCTCC No.:M 2021210 and named MS-FJ01. The total length of MS-FJ01 genome was 795 381 bp and the content of GC was 28.39%; The predicted number of coding genes was 704, accounting for 90.48% of the whole genome; 476, 382 and 425 genes were annotated in COG, GO and KEGG databases respectively. Through the analysis of each data, 40 drug resistance related genes, 4 carbohydrate related enzyme genes, 136 pathogen host interaction related genes, 47 virulence factor related genes, 106 membrane transporter related genes and 25 restriction modification related genes were annotated. Through further comparison with MS HN01 and MS 5-9 genomes, it was found that MS-FJ01 had a good collinear relationship with both Chinese strains, and was closer to MS 5-9. This study clarified the genomic structure of Fujian MS strain, predicted and annotated the function of its gene through the analysis of relevant databases, to provide a reference basis for the further study of MS.

Key words: Mycoplasma synoviae, isolation and identification, whole genome sequencing, gene function annotation analysis

CLC Number:

S852.62

XU Lifeng, JIA Chenyu, CHEN Fuzai, FANG Mengyuan, LIU Xiaodan, CHEN Jilong, LI Xunliang. Isolation, Identification and Whole Genome Analysis of a Mycoplasma synoviae Strain from Chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2663-2676.

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Isolation, Identification and Whole Genome Analysis of a Mycoplasma synoviae Strain from Chicken

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