布鲁氏菌OMP16对RAW264.7细胞凋亡与免疫活性的影响

doi:10.11843/j.issn.0366-6964.2022.08.022

Abstract

Abstract: The purpose of this study was to study the effect of Brucella outer membrane protein 16 on apoptosis and immune activity of mouse macrophage RAW264.7 cell, and to explore its mechanism. In this study, OMP16 treated RAW264.7 cells were used as a model to detect the effects of OMP16 on cell viability by MTT and Hoechst assay. The expressions of Caspase-3, GRP78 and CHOP level were detected by Western blot. The apoptosis of OMP16 treated RAW264.7 was detected by flow cytometry. The changes of IL-1β, IL-6 and TNF-α were detected by RT-PCR. The results showed that OMP16 could affect the activity of RAW264.7 cells in a concentration-dependent manner. OMP16 significantly increased the expression of caspase-3 (P<0.001) and promoted apoptosis of OMP16 treated RAW264.7 cells. The expression of ER stress marker protein GRP78 and CHOP was significantly increased in RAW264.7 cells after OMP16 treated 24 and 36 h. The mRNA expression levels of IL-1β, IL-6, TNF-α were significantly up-regulated. Brucella OMP16 can induce RAW264.7 apoptosis, and OMP16 significantly causes ER stress through CHOP pathway.

Key words: Brucella, outer membrane protein, OMP16, RAW264.7, apoptosis, immune activity

CLC Number:

S852.61

LI Yang, ZHOU Dong, YIN Yanlong, ZHANG Guangdong, XIANG Caixia, ZHI Feijie, BAI Furong, LIN Pengfei, JIN Yaping, WANG Aihua. Effects of Brucella Outer Membrane Protein 16 on Apoptosis and Immune Activity of RAW264.7 Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2642-2651.

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Effects of Brucella Outer Membrane Protein 16 on Apoptosis and Immune Activity of RAW264.7 Cells

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