干扰素刺激基因对羊口疮病毒在OFTu细胞中增殖的抑制效应

doi:10.11843/j.issn.0366-6964.2022.02.025

Abstract

Abstract: As an important zoonotic virus, orf virus (ORFV) not only seriously affects the sheep breeding, but also threatens human health. Stimulator of interferon genes (STING), as DNA sensor of the cell, plays an important role in innate immunity of the host. In order to explore the role of STING in infection and replication of ORFV, cell model of ORFV infection of ovine fetal turbinate cells (OFTu) was constructed and dynamic expression of STING and related genes after ORFV infection in cells were analyzed. The effects of STING on ORFV proliferation were investigated under the state of interference expression and overexpression in OFTu cells. The results showed that the transcription of STING, cGAS, TBK1, IRF3, IRF7, IL-6, IFN-β, IL-1β and TNF-α were significantly increased after OFTu cells infected with ORFV. OFTu cells with STING overexpression resulted in up-regulated transcription of RIG-Ⅰ,DDX41, IFI16, IRF3, IRF7, IL-6, TNF-α, IFN-α and IFN-β.Infection of OFTu cells with STING overexpression can increase the expression of TBK-1, IRF3, IFN-β and TNF-α genes and inhibit the replication of ORFV. While, infection of OFTu cells with the interference of STING expression can decrease the transcription of TBK-1, IRF3, IFN-β and TNF-α and increase the replication of ORFV. These results indicated that STING protein can enhance the expression of antiviral cytokines and inhibit the proliferation of ORFV in OFTu cells. The results provide scientific theoretical basis for further understanding the role of STING in infection and replication of ORFV, and provide basic data for further exploration of the molecular mechanism of ORFV infection and pathogenicity.

Key words: orf virus, STING, infection, replication

CLC Number:

S852.659.1

CHEN Yongjie, LIU Jianxin, LI Huizi, ZHONG Wenxia, LI Baojian, PI Molin, NING Zhangyong. Inhibitory Effect of Stimulator of Interferon Genes on the Proliferation of Orf Virus in OFTu Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 588-596.

References 32

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Inhibitory Effect of Stimulator of Interferon Genes on the Proliferation of Orf Virus in OFTu Cells

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