微囊化沙门菌SP4噬菌体的制备及其缓释特性分析

doi:10.11843/j.issn.0366-6964.2022.07.028

Abstract

Abstract: Microencapsulated SP4 phage was prepared by using cationic etherified starch/sodium alginate/xanthan gum/nano TiO₂/chitosan oligosaccharide as raw materials, to provide biological agents for controlling Salmonella infection. Salmonella SP4 phage was used to prepare microencapsulated SP4 phage by droplet method, and its titer, cationic etherified starch concentration, sodium alginate concentration, xanthan gum concentration, chitosan oligosaccharide concentration, pH stability, thermal stability, simulated gastric juice stability, simulated intestinal juice release behavior and preservation stability were analyzed. The results showed that when the concentration of cationic etherified starch was 2.4%, the concentration of sodium alginate was 2%, the concentration of xanthan gum was 1%, and the concentration of chitosan oligosaccharide was 0.6%, the shape of microencapsulated SP4 phage microspheres was regular, and the encapsulation efficiency was 97.5%. The phage maintained high biological activity in the range of pH 5.0-8.0, temperature 10-30℃, simulated gastric juice pH 2.0 for 0-30 min and pH 3.0 for 0-150 min. The phage was completely released after 4 h in simulated intestinal juice, and the phage titer did not decrease significantly after 6 weeks at 4℃. The results show that microencapsulated Salmonella SP4 phage significantly improves the stability and sustained-release performance of phage, its acid resistance is conducive to resisting the decomposition of gastric acid, and has the potential to be used in biological control, which lays a foundation for the further identification of microencapsulated Salmonella SP4 phage by multi factor test.

Key words: SP4 phage, microencapsulated coating, stability, slow release performance, biological agent

CLC Number:

S854.5

ZHANG Bo, ZHANG Yongying, HAO He, ZHANG Yibo, LV Xingbang, ZHU Shouchuang, ZHU Zhen, SHI Yuxiang. Preparation of Microencapsulated Salmonella SP4 Bacteriophage and the Study on Its Slow-release Performance[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2317-2325.

References 35

[1]	BURMEISTER A R, TURNER P E. Trading-off and trading-up in the world of bacteria-phage evolution[J]. Curr Biol, 2020, 30(19):R1120-R1124.
[2]	ZHANG W H, WU Q. Applications of phage-derived RNA-based technologies in synthetic biology[J]. Synth Syst Biotechnol, 2020, 5(4):343-360.
[3]	巩倩雯,李一昊,曾颃,等.特异性识别K1荚膜大肠杆菌的噬菌体PNJ1809-36生物学特性及全基因组分析[J].畜牧兽医学报, 2021, 52(6):1677-1688.GONG Q W, LI Y H, ZENG H, et al. Biological characteristics and whole genome analysis of phage PNJ1809-36 target Escherichia coli K1[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6):1677-1688.(in Chinese)
[4]	ESTEVINHO B N, HORCIU I L, BLAGA A C, et al. Development of controlled delivery functional systems by microencapsulation of different extracts of plants:Hypericum perforatum L., Salvia officinalis L. and Syzygium aromaticum[J]. Food Bioprocess Technol, 2021, 14(8):1503-1517.
[5]	PUTTARAT N, THANGRONGTHONG S, KASEMWONG K, et al. Spray-drying microencapsulation using whey protein isolate and nano-crystalline starch for enhancing the survivability and stability of Lactobacillus reuteri TF-7[J]. Food Sci Biotechnol, 2021, 30(2):245-256.
[6]	LI J J, LI Y M, DING Y F, et al. Characterization of a novel Siphoviridae Salmonella bacteriophage T156 and its microencapsulation application in food matrix[J]. Food Res Int, 2021, 140:110004.
[7]	GUO Y T, LI J, ISLAM S, et al. Application of a novel phage vB_SalS-LPSTLL for the biological control of Salmonella in foods[J]. Food Res Int, 2021, 147:110492.
[8]	龙门,周卉,谢文,等.海藻酸钠微囊化JS25噬菌体的制备、表征及其在食品模拟体系中的释放[J].食品科学, 2018, 39(12):262-267.LONG M, ZHOU B, XIE W, et al. Preparation, characterization and release characteristics in food simulant systems of sodium alginate microcapsules containing phage JS25[J]. Food Science, 2018, 39(12):262-267.(in Chinese)
[9]	张玉宇,张敏,黄赞,等. 1株跨属感染猪霍乱沙门菌和大肠杆菌烈性噬菌体的分离及其生物学特性[J].畜牧兽医学报, 2020, 51(8):2032-2038.ZHANG Y Y, ZHANG M, HUANG Z, et al. Isolation and characterization of a lytic polyvalent bacteriophage infecting Salmonella choleraesuis and Escherichia coli[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8):2032-2038.(in Chinese)
[10]	何依蓉,张奕杰,杨伟,等.鼠伤寒沙门菌烈性噬菌体的分离鉴定与生物学特性[J].畜牧兽医学报, 2021, 52(3):763-771.HE Y R, ZHANG Y J, YANG W, et al. Isolation, identification and biological properties of a lytic phage against Salmonella typhimurium[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3):763-771.(in Chinese)
[11]	马永生.口服微囊化噬菌体的制备及其在胃肠环境中的稳定性、释放行为和抗菌活性研究[D].大连:大连理工大学, 2011.MA Y S. Preparation of microencapsulated bacteriophages for oral delivery and evaluation of their stabilities, release behaviors and antimicrobial activities in the gastrointestinal environment[D]. Dalian:Dalian University of Technology, 2011.(in Chinese)
[12]	LAMAN A G, SHEPELYAKOVSKAYA A O, BROVKO F A, et al. Application of monoclonal antibodies and phage display technology for YB-1 protein analysis[J]. Russ J Bioorg Chem, 2020, 46(1):43-51.
[13]	TRIDGETT M, ABABI M, OSGERBY A, et al. Engineering bacteria to produce pure phage-like particles for gene delivery[J]. ACS Synth Biol, 2021, 10(1):107-114.
[14]	ALTAMIRANO F L G, BARR J J. Unlocking the next generation of phage therapy:the key is in the receptors[J]. Curr Opin Biotechnol, 2021, 68:115-123.
[15]	LAW N, ASLAM S. Phage therapy:primer and role in the treatment of MDROs[J]. Curr Infect Dis Rep, 2020, 22(12):31.
[16]	LI M, XIAO Y Y, LI P, et al. Characterization and genome analysis of Klebsiella phage P509, with lytic activity against clinical carbapenem-resistant Klebsiella pneumoniae of the KL64 capsular type[J]. Arch Virol, 2020, 165(12):2799-2806.
[17]	朱守创.益生菌LGG微胶囊化及对提高其存活性的机制研究[D].郑州:郑州大学, 2013.ZHU S C. Study on the microencapsulation of Lactobacillus rhamnosus GG and the mechanism behind the viability enhancement[D]. Zhengzhou:Zhengzhou University, 2013.(in Chinese)
[18]	吕小丽.阳离子氧化微孔大米淀粉的制备、性能及应用研究[D].沈阳:沈阳工业大学, 2020.LV X L. Cationic oxidized microporous rice starch:preparation, characterization, and properties[D]. Shenyang:Shenyang University of Technology, 2020.(in Chinese)
[19]	梁冬梅,莫福旺,黄志强,等.阳离子醚化淀粉改性脲醛树脂胶黏剂的研究[J].化工技术与开发, 2020, 49(8):17-19, 22.LIANG D M, MO F W, HUANG Z Q, et al. Study on cationic etherified starch modified urea-formaldehyde resin adhesive[J]. Technology&Development of Chemical Industry, 2020, 49(8):17-19, 22.(in Chinese)
[20]	周蕊.微/纳淀粉材料的制备、表征及其与两种食品成分的相互作用性能研究[D].武汉:武汉轻工大学, 2019.ZHOU R. Study on preparation and characterization of Micro/Nano starch materials and their interaction with two kinds of food components[D]. Wuhan:Wuhan Polytechnic University, 2019.(in Chinese)
[21]	赵凯强,杨超,王晨.阳离子改性淀粉絮凝剂的研究进展[J].当代化工, 2019, 48(9):2162-2166.ZHAO K Q, YANG C, WANG C. Research progress of cationic modified starch flocculant[J]. Contemporary Chemical Industry, 2019, 48(9):2162-2166.(in Chinese)
[22]	YANG I H, CHEN Y S, LI J J, et al. The development of laminin-alginate microspheres encapsulated with Ginsenoside Rg1 and ADSCs for breast reconstruction after lumpectomy[J]. Bioact Mater, 2021, 6(6):1699-1710.
[23]	CASTRO-YOBAL M A, CONTRERAS-OLIVA A, SAUCEDO-RIVALCOBA V, et al. Evaluation of physicochemical properties of film-based alginate for food packing applications[J]. e-Polymers, 2021, 21(1):82-95.
[24]	DMITRENKO M, LIAMIN V, LAHDERANTA E, et al. Mixed matrix membranes based on sodium alginate modified by fullerene derivatives with L-amino acids for pervaporation isopropanol dehydration[J]. J Mater Sci, 2021, 56(12):7765-7787.
[25]	REHMAN S, RANJHA N M, RAZA M R, et al. Enteric-coated Ca-alginate hydrogel beads:a promising tool for colon targeted drug delivery system[J]. Polym Bull, 2021, 78(9):5103-5117.
[26]	FENG Z Y, XU J, NI C H. Preparation of redox responsive modified xanthan gum nanoparticles and the drug controlled release[J]. Int J Polym Mater Polym Biomat, 2021, 70(14):994-1001.
[27]	JIAO T, LIAN Q, ZHAO T Z, et al. Preparation, mechanical and biological properties of inkjet printed alginate/gelatin hydrogel[J]. J Bionic Eng, 2021, 18(3):574-583.
[28]	WANG Y C, ZHU W G, YAO T K, et al. Nanoscale TiO2 coating improves water stability of Cs2SnCl6[J]. MRS Commun, 2020, 10(4):687-694.
[29]	VEIGA E T, FERNANDES S L, DE OLIVEIRA GRAEFF C F, et al. Compact TiO2 blocking-layer prepared by LbL for perovskite solar cells[J]. Solar Energy, 2021, 214:510-516.
[30]	韩雪.纳米TiO2促进噬菌体介导的抗生素抗性基因水平转移机制研究[D].镇江:江苏大学, 2019.HAN X. Study on the mechanism of Nano-TiO2 promoting phage-mediated horizontal transfer of antibiotic resistance genes[D]. Zhenjiang:Jiangsu University, 2019.(in Chinese)
[31]	BAI R B, YU Y Y, WANG Q, et al. Laccase-catalyzed polymerization of hydroquinone incorporated with chitosan oligosaccharide for enzymatic coloration of cotton[J]. Appl Biochem Biotechnol, 2020, 191(2):605-622.
[32]	MBAE K M, UMESHA S. Physicochemical and antimicrobial properties of post-synthesis betanin and chitosan oligosaccharide functionalized silver nanoparticles[J]. J Nanopart Res, 2020, 22(11):346.
[33]	YU Z D, SCHWARZ C, ZHU L, et al. Hitchhiking behavior in bacteriophages facilitates phage infection and enhances carrier bacteria colonization[J]. Environ Sci Technol, 2021, 55(4):2462-2472.
[34]	MOON K, CHO J C. Metaviromics coupled with phage-host identification to open the viral'black box'[J]. J Microbiol, 2021, 59(3):311-323.
[35]	ISHIDA N, HOSOKAWA Y, IMAEDA T, et al. Reduction of the cytotoxicity of copper (II) oxide nanoparticles by coating with a surface-binding peptide[J]. Appl Biochem Biotechnol, 2020, 190(2):645-659.

Preparation of Microencapsulated Salmonella SP4 Bacteriophage and the Study on Its Slow-release Performance

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 35

Related Articles 12

Recommended Articles

Metrics

Comments

[1]	YU Xiuju, ZHANG Min'ai, HU Yanjiao, ZHU Zhiwei, WANG Haidong, YANG Lihua, FAN Kuohai. Isolation, Expression and Stability Analysis of Bacteriocins from Bacillus subtilis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 323-333.
[2]	LI Rang, WENG Xiang, LI Quanxiao, WU Daocheng, CAO Hui, ZHANG Ailian. Analysis of Emulsifying Method and Stability of Foot-and-mouth Disease Vaccine Combined with Crude Polysaccharides from Cultivated Artemisia rupestris L. [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1608-1615.
[3]	ZHAO Yaya, CHANG Jiang, JU Ning, LUO Yulong. Mechanism of Plant Polyphenols Addition in Diet Regulating Meat Color Stability [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 36-47.
[4]	LI Hui, XIONG Jing, MEI Cui, WANG Shiyuan, ZHOU Yang, LI Xiaofen, FU Guihua, ZHANG Yang, CHENG Peng, HE Yuzhang, CHEN Hongwei. The Safety and Stability of the Antimicrobial Peptide CRAMP and Its Role in Eradicating Pseudomonas aeruginosa Biofilms [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1576-1586.
[5]	YANG Xianying, XIONG Xianrong, HAN Jie, HUANG Xiangyue, WANG Yan, A GUO Yueda, LI Jian. Identification of Suitable Reference Gene for Quantitative RT-PCR Analysis in Mouse Ovaries [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 446-453.
[6]	CHEN Rui，YANG Xiao-nong，ZENG Wan-qiu，WEN Juan. Expression Stability Analysis of Reference Genes in Different Development Periods and Tissues in Oryctolagus Cuniculus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 477-483.
[7]	WEI Xiao-xiao，HANG Bo-lin，MA Cui，XIA Yi-he，ZHANG Bing-qing，WANG Qing，XU Yan-zhao，HU Jian-he. Analysis of Antimicrobial Activity and Stability of Antibacterial Peptide JH-3 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 361-366.
[8]	LIU Hui，BU Deng-pan，Lü Zhong-wang，LI Fa-di，LIU Shi-jie，ZHANG Kai-zhan，WANG Jia-qi. Effect of Lactic Acid Bacteria or a Chemical Preservative on the Quality and Aerobic Stability of Alfalfa Silage Produced in Farm-scale Silos [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 784-791.
[9]	CHEN Lei，YUAN Xian-jun，GUO Gang，WEN Ai-you，XIAO Shen-hua,BA Sang，YU Cheng-qun，SHAO Tao. The Effects of Lactic Acid Bacteria and Propionic Acid on the Fermentation Quality and Aerobic Stability of Total Mixed Ration Silages Prepared with Whole-crop Corn in Tibet [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 104-110.
[10]	CHEN Li，ZHAO Wei，ZHAN Si-yuan，LI Dan，LI Li，ZHONG Tao，WANG Lin-jie，ZHANG Hong-ping. The Expression Stability Analysis of Reference Genes in the Different Tissues and Skeletal Muscle of Different Development Periods in Goat [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(8): 1228-1236.
[11]	LIANG Jian-guang;LV Lin;LUO Xu-gang;DIAO Qi-yu;LIU Bin;. Physical and Chemical Characteristics of Supplemental Organic Zinc Sources and Their Stabilities in vitro Fermentation Rumen [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(10): 1355-1366.
[12]	JIN Zhong-hui; XIAO Xi-long;XUE Zhan-yong. Studies on the Combination Injectable Solution of Dandelion [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2005, 36(4): 402-406.