奶牛早孕诊断技术研究进展

doi:10.11843/j.issn.0366-6964.2022.07.002

Abstract

Abstract: High reproductive rate is an important guarantee for enhancing the economic benefits of dairy farms, and early pregnancy diagnosis is one of the important means in improving the reproductive rate of dairy cows. It is difficult for traditional pregnancy diagnosis methods to determine the pregnancy status of dairy cows within 21 days after mating. However, the new pregnancy diagnosis technologies developed in recent years can effectively diagnose early pregnancy in dairy cows. In this paper, color Doppler ultrasound, mid-infrared spectroscopy technology, and pregnancy-related protein, circulating nucleotides, interferon tau-induced gene detection and other technologies about the latest diagnosis techniques of early pregnancy in dairy cows were reviewed. Meanwhile, the practical application effects, the advantages and disadvantages of these technologies were compared. And the new diagnostic techniques for early pregnancy were predicted in dairy cows, which would provide some ideas and references for exploitation and application of advanced and high-efficiency early pregnancy diagnosis techniques in dairy cows.

Key words: cow, diagnosis of early pregnancy, pregnancy associated protein, infrared spectrum, circulating nucleic acid

CLC Number:

S823.913

WEI Shihao, DAI Tianshu, WANG Feng, SHI Yuangang, MA Yun, DAN Xingang. Research Progress of Early Pregnancy Diagnosis in Dairy Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2047-2056.

References 71

[1]	FERNANDES C A D C,PEREIRA J R,SOUZA V O,et al.Timing of early resynchronization protocols affects subsequent pregnancy outcome in dairy cows[J].Theriogenology,2021,167:61-66.
[2]	SZENCI O.Recent possibilities for the diagnosis of early pregnancy and embryonic mortality in dairy cows[J].Animals (Basel),2021,11(6):1666.
[3]	HOLTZ W,NIGGEMEYER H.Reliable identification of pregnant dairy cows by double milk progesterone analysis[J].Livest Sci,2019,228:38-41.
[4]	BARBATO O,MENCHETTI L,SOUSA N M,et al.Pregnancy-associated glycoproteins (PAGs) concentrations in water buffaloes (Bubalus bubalis) during gestation and the postpartum period[J].Theriogenology,2017,97:73-77.
[5]	BOND R L,MIDLA L T,GORDON E D,et al.Effect of student transrectal palpation on early pregnancy loss in dairy cattle[J].J Dairy Sci,2019,102(10):9236-9240.
[6]	ROMANO J E,PINEDO P,BRYAN K,et al.Comparison between allantochorion membrane and amniotic sac detection by per rectal palpation for pregnancy diagnosis on pregnancy loss,calving rates,and abnormalities in newborn calves[J].Theriogenology,2017,90:219-227.
[7]	ROMANO J E,BRYAN K,RAMOS R S,et al.Effect of early pregnancy diagnosis by per rectum amniotic sac palpation on pregnancy loss,calving rates,and abnormalities in newborn dairy calves[J].Theriogenology,2016,85(3):419-427.
[8]	KANAZAWA T,SEKI M,ISHIYAMA K,et al.Pregnancy prediction on the day of embryo transfer (Day 7) and Day 14 by measuring luteal blood flow in dairy cows[J].Theriogenology,2016,86(6):1436-1444.
[9]	HASSAN M,ARSHAD U,BILAL M,et al.Luteal blood flow measured by Doppler ultrasonography during the first three weeks after artificial insemination in pregnant and non-pregnant Bos indicus dairy cows[J].J Reprod Dev,2019,65(1):29-36.
[10]	DUBUC J,HOULE J,ROUSSEAU M,et al.Short communication:Accuracy of corpus luteum color flow Doppler ultrasonography to diagnose nonpregnancy in dairy cows on day 21 after insemination[J].J Dairy Sci,2020,103(2):2019-2023.
[11]	UTT M D,JOHNSON G L,BEAL W E.The evaluation of corpus luteum blood flow using color-flow Doppler ultrasound for early pregnancy diagnosis in bovine embryo recipients[J].Theriogenology,2009,71(4):707-715.
[12]	SIQUEIRA L G B,AREAS V S,GHETTI A M,et al.Color Doppler flow imaging for the early detection of nonpregnant cattle at 20 days after timed artificial insemination[J].J Dairy Sci,2013,96(10):6461-6472.
[13]	ANDRADE J P N,ANDRADE F S,GUERSON Y B,et al.Early pregnancy diagnosis at 21 days post artificial insemination using corpus luteum vascular perfusion compared to corpus luteum diameter and/or echogenicity in Nelore heifers[J].Anim Reprod Sci,2019,209:106144.
[14]	LASHEEN M E,BADR H M,KANDIEL M M M,et al.Predicting early pregnancy in Egyptian buffalo cows via measuring uterine and luteal blood flows,and serum and saliva progesterone[J].Trop Anim Health Prod,2018,50(1):137-142.
[15]	HONNENS A,VOSS C,HERZOG K,et al.Uterine blood flow during the first 3 weeks of pregnancy in dairy cows[J].Theriogenology,2008,70(7):1048-1056.
[16]	SAMIR H,KANDIEL M M M.Accuracy of subjective evaluation of luteal blood flow by color Doppler ultrasonography for early diagnosis of pregnancy in Egyptian buffalo[J].Anim Reprod Sci,2019,208:106129.
[17]	SCULLY S,BUTLER S T,KELLY A K,et al.Early pregnancy diagnosis on days 18 to 21 postinsemination using high-resolution imaging in lactating dairy cows[J].J Dairy Sci,2014,97(6):3542-3557.
[18]	PANARACE M,GARNIL C,MARFIL M,et al.Transrectal Doppler sonography for evaluation of uterine blood flow throughout pregnancy in 13 cows[J].Theriogenology,2006,66(9):2113-2119.
[19]	LAINÉ A,BASTIN C,GRELET C,et al.Assessing the effect of pregnancy stage on milk composition of dairy cows using mid-infrared spectra[J].J Dairy Sci,2017,100(4):2863-2876.
[20]	HO P N,PRYCE J E.Predicting the likelihood of conception to first insemination of dairy cows using milk mid-infrared spectroscopy[J].J Dairy Sci,2020,103(12):11535-11544.
[21]	HO P N,BONFATTI V,LUKE T D W,et al.Classifying the fertility of dairy cows using milk mid-infrared spectroscopy[J].J Dairy Sci,2019,102(11):10460-10470.
[22]	DELHEZ P,HO P N,GENGLER N,et al.Diagnosing the pregnancy status of dairy cows:How useful is milk mid-infrared spectroscopy?[J].J Dairy Sci,2020,103(4):3264-3274.
[23]	TOLEDO-ALVARADO H,VAZQUEZ A I,DE LOS CAMPOS G,et al.Diagnosing pregnancy status using infrared spectra and milk composition in dairy cows[J].J Dairy Sci,2018,101(3):2496-2505.
[24]	SPENCER T E,FORDE N,LONERGAN P.The role of progesterone and conceptus-derived factors in uterine biology during early pregnancy in ruminants[J].J Dairy Sci,2016,99(7):5941-5950.
[25]	YOSHINO H,KIZAKI K,IGA K,et al.Use of a prediction method for early pregnancy status utilizing receiver operating characteristic curve analysis of peripheral blood leukocyte interferon-stimulated genes in Japanese-Black cattle[J].Anim Reprod Sci,2020,214:106283.
[26]	YOSHINO H,TOJI N,SASAKI K,et al.A predictive threshold value for the diagnosis of early pregnancy in cows using interferon-stimulated genes in granulocytes[J].Theriogenology,2018,107:188-193.
[27]	HAQ I U,HAN Y,ALI T,et al.Expression of interferon-stimulated gene ISG15 and ubiquitination enzymes is upregulated in peripheral blood monocyte during early pregnancy in dairy cattle[J].Reprod Biol,2016,16(4):255-260.
[28]	GREEN J C,OKAMURA C S,POOCK S E,et al.Measurement of interferon-tau (IFN-τ) stimulated gene expression in blood leukocytes for pregnancy diagnosis within 18-20 d after insemination in dairy cattle[J].Anim Reprod Sci,2010,121(1-2):24-33.
[29]	KOWALCZYK A,CZERNIAWSKA-PITKOWSKA E,WRZECIN'SKA M.The importance of interferon-tau in the diagnosis of pregnancy[J].Biomed Res Int,2021,2021:9915814.
[30]	KIKUCHI M,KIZAKI K,SHIGENO S,et al.Newly identified interferon tau-responsive Hes family BHLH transcription factor 4 and cytidine/uridine monophosphate kinase 2 genes in peripheral blood granulocytes during early pregnancy in cows[J].Domest Anim Endocrinol,2019,68:64-72.
[31]	RUHMANN B,GILLER K,HANKELE A K,et al.Interferon-τ induced gene expression in bovine hepatocytes during early pregnancy[J].Theriogenology,2017,104:198-204.
[32]	DALMASO DE MELO G,MELLO B P,FERREIRA C A,et al.Applied use of interferon-tau stimulated genes expression in polymorphonuclear cells to detect pregnancy compared to other early predictors in beef cattle[J].Theriogenology,2020,152:94-105.
[33]	BATRA K,KUMAR A,MAAN S,et al.Recombinant interferon stimulated protein 15(rISG15) as a molecular marker for detection of early pregnancy in Bubalus bubalis[J].Anim Reprod Sci,2018,197:106-116.
[34]	PANDA B S K,MOHAPATRA S K,CHAUDHARY D,et al.Proteomics and transcriptomics study reveals the utility of ISGs as novel molecules for early pregnancy diagnosis in dairy cows[J].J Reprod Immunol,2020,140:103148.
[35]	ZHANG T,GUO S,ZHOU H,et al.Endometrial extracellular matrix rigidity and IFNτ ensure the establishment of early pregnancy through activation of YAP[J].Cell Prolif,2021,54(2):e12976.
[36]	POHLER K G,REESE S T,FRANCO G A,et al.New approaches to diagnose and target reproductive failure in cattle[J].Anim Reprod,2020,17(3):e20200057.
[37]	MAYER J,SOLLER J T,BECK J,et al.Early pregnancy diagnosis in dairy cows using circulating nucleic acids[J].Theriogenology,2013,79(1):173-179.
[38]	GHAFOURI-FARD S,SHOOREI H,TAHERI M.The role of microRNAs in ectopic pregnancy:A concise review[J].Noncoding RNA Res,2020,5(2):67-70.
[39]	IOANNIDIS J,DONADEU F X.Circulating miRNA signatures of early pregnancy in cattle[J].BMC Genomics,2016,17(1):184.
[40]	TESFAYE D,WORKU D,RINGS F,et al.Identification and expression profiling of microRNAs during bovine oocyte maturation using heterologous approach[J].Mol Reprod Dev,2009,76(7):665-677.
[41]	SU L J,ZHAO S H,ZHU M J,et al.Differential expression of microRNAs in porcine placentas on days 30 and 90 of gestation[J].Reprod Fertil Dev,2010,22(8):1175-1182.
[42]	GUELFI G,STEFANETTI V,DE LUCA S,et al.Serum microRNAs in buffalo cows:potential biomarkers of pregnancy[J].Res Vet Sci,2017,115:294-300.
[43]	SCHANZENBACH C I,KIRCHNER B,ULBRICH S E,et al.MicroRNA of whole milk samples are not suitable for pregnancy detection in cattle[J].Gene,2019,692:17-21.
[44]	GEBREMEDHN S,SALILEW-WONDIM D,HOELKER M,et al.Exploring maternal serum microRNAs during early pregnancy in cattle[J].Theriogenology,2018,121:196-203.
[45]	ZHAO G,GUO S,JIANG K F,et al.MiRNA profiling of plasma-derived exosomes from dairy cows during gestation[J].Theriogenology,2019,130:89-98.
[46]	REESE S T,PEREIRA M H C,EDWARDS J L,et al.Pregnancy diagnosis in cattle using pregnancy associated glycoprotein concentration in circulation at day 24 of gestation[J].Theriogenology,2018,106:178-185.
[47]	GATEA A O,SMITH M F,POHLER K G,et al.The ability to predict pregnancy loss in cattle with ELISAs that detect pregnancy associated glycoproteins is antibody dependent[J].Theriogenology,2018,108:269-276.
[48]	NORTHROP E J,RICH J J J,RHOADES J R,et al.Comparison of two bovine serum pregnancy tests in detection of artificial insemination pregnancies and pregnancy loss in beef cattle[J].PLoS One,2019,14(1):e211179.
[49]	FOSGATE G T,MOTIMELE B,GANSWINDT A,et al.A Bayesian latent class model to estimate the accuracy of pregnancy diagnosis by transrectal ultrasonography and laboratory detection of pregnancy-associated glycoproteins in dairy cows[J].Prev Vet Med,2017,145:100-109.
[50]	MERCADANTE P M,RIBEIRO E S,RISCO C,et al.Associations between pregnancy-associated glycoproteins and pregnancy outcomes,milk yield,parity,and clinical diseases in high-producing dairy cows[J].J Dairy Sci,2016,99(4):3031-3040.
[51]	BARBATO O,GUELFI G,BARILE V L,et al.Using real-time PCR to identify pregnancy-associated glycoprotein 2(PAG-2) in water buffalo (Bubalus bubalis) blood in early pregnancy[J].Theriogenology,2017,89:106-113.
[52]	DUFOUR S,DUROCHER J,DUBUC J,et al.Bayesian estimation of sensitivity and specificity of a milk pregnancy-associated glycoprotein-based ELISA and of transrectal ultrasonographic exam for diagnosis of pregnancy at 28-45 days following breeding in dairy cows[J].Prev Vet Med,2017,140:122-133.
[53]	AYAD A,SOUSA N M,SULON J,et al.Correlation of five radioimmunoassay systems for measurement of bovine plasma pregnancy-associated glycoprotein concentrations at early pregnancy period[J].Res Vet Sci,2009,86(3):377-382.
[54]	POHLER K G,PEREIRA M H C,LOPES F R,et al.Circulating concentrations of bovine pregnancy-associated glycoproteins and late embryonic mortality in lactating dairy herds[J].J Dairy Sci,2016,99(2):1584-1594.
[55]	KAREN A,SOUSA N M D,BECKERS J F,et al.Comparison of a commercial bovine pregnancy-associated glycoprotein ELISA test and a pregnancy-associated glycoprotein radiomimmunoassay test for early pregnancy diagnosis in dairy cattle[J].Anim Reprod Sci,2015,159:31-37.
[56]	MOUSSAFIR Z,ALLAI L,EL KHALIL K,et al.Could a bovine pregnancy rapid test be an alternative to a commercial pregnancy-associated glycoprotein ELISA test in dairy cattle?[J].Anim Reprod Sci,2018,192:78-83.
[57]	BRAGANÇA G M,MONTEIRO B M,ALBUQUERQUE R D S,et al.Using pregnancy-associated glycoproteins to provide early pregnancy diagnosis in Nelore cows[J].Livest Sci,2018,214:278-281.
[58]	SILVA E,STERRY R A,KOLB D,et al.Accuracy of a pregnancy-associated glycoprotein ELISA to determine pregnancy status of lactating dairy cows twenty-seven days after timed artificial insemination[J].J Dairy Sci,2007,90(10):4612-4622.
[59]	LAWSON B C,SHAHZAD A H,DOLECHECK K A,et al.A pregnancy detection assay using milk samples:Evaluation and considerations[J].J Dairy Sci,2014,97(10):6316-6325.
[60]	HAN R X,KIM H R,DIAO Y F,et al.Detection of early pregnancy-specific proteins in Holstein milk[J].J Proteomics,2012,75(11):3221-3229.
[61]	SINEDINO L D P,LIMA F S,BISINOTTO R S,et al.Effect of early or late resynchronization based on different methods of pregnancy diagnosis on reproductive performance of dairy cows[J].J Dairy Sci,2014,97(8):4932-4941.
[62]	RICCI A,CARVALHO P D,AMUNDSON M C,et al.Factors associated with pregnancy-associated glycoprotein (PAG) levels in plasma and milk of Holstein cows during early pregnancy and their effect on the accuracy of pregnancy diagnosis[J].J Dairy Sci,2015,98(4):2502-2514.
[63]	MAYO L M,MOORE S G,POOCK S E,et al.Technical note:Validation of a chemical pregnancy test in dairy cows that uses whole blood,shortened incubation times,and visual readout[J].J Dairy Sci,2016,99(9):7634-7641.
[64]	ALKAN H,KIVRAK M B,SATILMIS F,et al.Detection of twin pregnancies in ewes by pregnancy-associated glycoprotein assay and transabdominal ultrasonography[J].Domest Anim Endocrinol,2020,72:106399.
[65]	MIDDLETON E L,PURSLEY J R.Short communication:Blood samples before and after embryonic attachment accurately determine non-pregnant lactating dairy cows at 24 d post-artificial insemination using a commercially available assay for pregnancy-specific protein B[J].J Dairy Sci,2019,102(8):7570-7575.
[66]	FILHO R V O,FRANCO G A,REESE S T,et al.Using pregnancy associated glycoproteins (PAG) for pregnancy detection at day 24 of gestation in beef cattle[J].Theriogenology,2020,141:128-133.
[67]	REESE S T,GEARY T W,FRANCO G A,et al.Pregnancy associated glycoproteins (PAGs) and pregnancy loss in high vs sub fertility heifers[J].Theriogenology,2019,135:7-12.
[68]	ROCHA C C,ANDRADE S C D S,DE MELO G D,et al.Early pregnancy-induced transcripts in peripheral blood immune cells in Bos indicus heifers[J].Sci Rep,2020,10(1):13733.
[69]	OTT T L.Symposium review:Immunological detection of the bovine conceptus during early pregnancy[J].J Dairy Sci,2019,102(4):3766-3777.
[70]	HU K L,ZHAO H C,YU Y,et al.Kisspeptin as a potential biomarker throughout pregnancy[J].Eur J Obstet Gynecol Reprod Biol,2019,240:261-266.
[71]	HU K L,ZHANG Y L,YANG Z,et al.Predictive value of serum kisspeptin concentration at 14 and 21 days after frozen-thawed embryo transfer[J].Reprod Biomed Online,2019,39(1):161-167.

Research Progress of Early Pregnancy Diagnosis in Dairy Cows

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