系统分析多组织转录组鉴定影响猪脂肪沉积的关键基因

doi:10.11843/j.issn.0366-6964.2022.06.005

Abstract

Abstract: This study aimed to identify key genes affecting fat deposition and explore the function of adipose tissue, liver and muscle in Songliao black pigs. In this study, 6 healthy Songliao black pigs with weight of about 100 kg and significant difference in backfat thickness (3 high and 3 low) were selected as experimental animals. The gene expression levels in fat, liver and longissimus dorsi muscle were detected by high-throughput transcriptome sequencing, the differentially expressed genes in pigs with different fat deposition levels and different tissues were identified, and the biological functions of the differentially expressed genes were analyzed. The results showed that 135 differentially expressed genes were found in different groups of pigs, some of which were involved in PPAR signaling pathway, AMPK signaling pathway, metabolic pathway, fatty acid metabolism and glycerol metabolism. The biological function analysis showed that EHHADH, ME1, SCD, OLR1, PHGDH, ACLY, LEP and CYP gene family were the key genes affecting pig fat deposition. Among the differentially expressed genes in different tissues, the highly expressed genes in adipose tissue were significantly enriched in insulin signaling pathway, MAPK signaling pathway, tricarboxylic acid cycle, oxidative phosphorylation pathways, and so on; The highly expressed genes in the liver were significantly enriched in the metabolism of various substances, the degradation of fatty acids and the synthesis of amino acids; The highly expressed genes in longissimus dorsi muscle were mainly involved in protein degradation, PI3K-Akt signaling pathway, oxidative phosphorylation pathway, Wnt signaling pathway and phosphatidylinositol signaling pathway. The analysis results of differentially expressed genes among different groups suggest that EHHADH, ME1, SCD, OLR1, PHGDH, ACLY, LEP and CYP gene family are the candidate genes affecting fat deposition; The differentially expressed genes between different tissues show that adipose tissue is the main site of adipogenesis, while liver and muscle tissue are mainly involved in the degradation of fatty acids. This study results has certain significance for genetic improvement and mechanism analysis of fat traits.

Key words: Songliao black pig, multi-tissue, transcriptome, system analysis, fat deposition

CLC Number:

S828.2

ZHAI Liwei, ZHAO Yanhui, LI Wenjun, XING Kai, WANG Chuduan. System Analysis of Multi Tissue Transcriptome to Identify Key Genes Affecting Porcine Fat Deposition[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1702-1711.

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System Analysis of Multi Tissue Transcriptome to Identify Key Genes Affecting Porcine Fat Deposition

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