2017—2021年成都市猫细小病毒的检测及其VP2基因的变异分析

doi:10.11843/j.issn.0366-6964.2022.04.031

Abstract

Abstract: In order to investigate the prevalence and genetic variation of feline parvovirus (FPV) in domestic cats in Chengdu region. From 2017 to 2021, 168 diarrheal fecal samples were collected from domestic cats in Chengdu region of Sichuan province. FPV was detected in 168 samples, and 13 positive samples’nucleotide sequence of VP2 genes were obtained. The homology and variation of FPV nucleotide and amino acid (aa) sequences was analyzed by MegAlign software, and the phylogenetic tree was constructed by neighbor-joining method through MEGA 7.0. Of the 168 samples examined, 70.2% (118/168, 95% CI=62.7%-77.0%) samples were positive for FPV. Sequence homology analysis showed that the 13 full-length VP2 genes shared 97.1% to 100% homology to reference strains. The aa sequence of SMU-D18 and SMU-D14 strains were consistent with new CPV-2a from cats. Interestingly, the SMU-D46 strain showed a unique substitution from Ser (S) to Phe (F) at both 293aa and 297aa. Furthermore, phylogenetic analysis showed that the FPV strains from China and other countries clustered into three subgroups (G1, G2 and G3). The FPV isolates obtained in this study were located in G1 and G3, respectively. It was worth noting that the commercial FPV vaccine strain was located in G2, which had distantly related to the FPV strains circulating in China. The results demonstrated a very high infectious rate of FPV among cats in Chengdu region. The protective efficiency of the existing commercial vaccines should be re-evaluated as soon as possible.

Key words: feline parvovirus, molecular epidemiology, VP2 gene, variant

CLC Number:

ZHOU Qun, YANG Yuan, SONG Xin, HE Xinyi, CAO Hui, ZHANG Bin. Detection of Feline Parvovirus and Mutation Analysis of VP2 Gene in Chengdu from 2017 to 2021[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1303-1309.

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Detection of Feline Parvovirus and Mutation Analysis of VP2 Gene in Chengdu from 2017 to 2021

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