猪hnRNPUL1基因克隆及变异剪接体鉴定

doi:10.11843/j.issn.0366-6964.2020.03.005

Abstract

Abstract: The study was conducted to clone the complete coding sequence (CDS) and transcript variants of porcine hnRNPUL1, and to analyze its tissue expression and subcellular localization. In this study, three 3-month-old Min pigs were used. The CDS and transcript variants of porcine hnRNPUL1 were cloned using RT-PCR method, and the expression was detected in tissues including heart, liver, spleen, lung, kidney, stomach, large intestine, small intestine, back muscle and leg muscle by qRT-PCR. At the same time, the subcellular localization was analyzed by inserting the fragments of CDS into pEGFP-N1 vector on the basis of bioinformatics prediction. It was found that the CDS of porcine hnRNPUL1 gene (GenBank accession No.:MN399154) was 2 580 bp of length, and the predicted polypeptide contained SAP, SPRY, and AAA domains, the characteristic domains of hnRNPs family. Porcine hnRNPUL1 was ubiquitously expressed in all detected tissues, with the highest level in the spleen but the lowest in leg muscle. The nuclear localization sequence(NLS) was located at 133-430 aa of the polypeptide chain, which was different from the result predicted by the bioinformatics methods. In addition, 2 splice variants and 11 alternative splicing fragments were identified. The results have provided the basis for further revealing the function and transcriptional regulation mechanism of porcine hnRNPUL1.

Key words: pig, hnRNPUL1, cloning, splice variants

CLC Number:

S828.2

SONG Yanfang, ZHANG Caixia, DU Fangfang, MA Junjie, LIU Dongyu, LIU Di, YANG Xiuqin. Cloning and Identification of Splice Variants of the Porcine hnRNPUL1 Gene[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 443-451.

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Cloning and Identification of Splice Variants of the Porcine hnRNPUL1 Gene

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