新疆地区牛冠状病毒的分子流行病学调查

doi:10.11843/j.issn.0366-6964.2023.12.022

Abstract

Abstract: Bovine coronavirus (BCoV) is one of the major pathogens causing diarrhea in calves. With the rapid development of cattle production in Xinjiang and the increase number of introduced cattle breeds, BCoV infection is on the rise, but there is a lack of comprehensive understanding of its epidemiological status and patterns. In this study a two-year follow-up survey from 2020 to 2022 on the prevalence of BCoV infection was conducted in the main cattle production areas of Xinjiang. In total 652 fecal samples of diarrhea calves were collected and tested for BCoV by RT-PCR. Genetic evolution analysis was performed on the isolated and identified strains. The results indicated that, the total positive rate of BCoV was 23.93% (156/652), in which it varied seasonally, with the peak in the winter being as high as 50.85%. The incidence of BCoV was higher in the southern than in the northern Xinjiang. Compared with dairy calves, meat calves were more susceptible to infection. The phylogenetic analysis showed that the S gene of BCoV/China/XJ-CJ/2022 isolated in this experiment was most closely in relation to the 2018 strain MN982199.1, the N gene was in relation to the 2018 strain MK095169.1 BCOV-China/SWUN/LN4/2018, the M gene was in relation to the 2017 strain MK095148.1 China/SWUN/SC1/2017, and the HE gene was in relation to the 2017 strain MK095136.1 BCOV-China/SWUN/SC2/2017. There was no genetic recombination occurred between BCoV/China/XJ-CJ/2022 and the BCoV strains from other countries. After nucleotide and amino acid similarity analysis of five structural proteins, the results showed that the genes were still the most similar to the local strain FJ938064.1_E-AH187-TC in China, reaching the lowest 91.50% and 96.70%. The results suggested that the seasonal, geographical and interbreed variability of BCoV infection in the scaled cattle farms in Xinjiang could provide data support for the development of the accurate prevention strategies.

Key words: bovine coronavirus, molecular epidemiology, genetic evolutionary analysis

CLC Number:

S852.653

WANG Mengjiao, JIANG Qian, MA Xuejun, XIA Ruiyang, GUO Xueping, SUN Lei, ZHONG Qi, MA Xuelian, YAO Gang. Molecular Epidemiological Investigation of Bovine Coronavirus in Calf Diarrhea in Main Cattle Producing Areas of Xinjiang[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5125-5133.

References

[1] LORENZETTI E,DE ARRUDA LEME R,RIBEIRO J,et al.Diarreia neonatal por coronavírus bovino (BCoV) em rebanhos bovinos de corte[J].Semina Ciências Agrárias,2013,34(6Supl2):3795-3800.
[2] 吴静,刘涵棋,白婷,等.新疆部分地区犊牛腹泻主要病原调查及分离菌耐药性分析[J].黑龙江畜牧兽医,2022(20):86-92,143.WU J,LIU H Q,BAI T,et al.Investigation on main pathogens of calf diarrhea in some areas of Xinjiang Uygur Autonomous Region and analysis of drug resistance of the isolates[J].Heilongjiang Animal Science and Veterinary Medicine,2022(20):86-92,143.(in Chinese)
[3] 王凡,马雪连,孙亚伟,等.新疆南疆地区部分集约化肉牛繁育场主要临床病症和生物安全调查[J].中国动物检疫,2022,39(7):22-28.WANG F,MA X L,SUN Y W,et al.Investigation on major clinical symptoms and biosafety in some intensive beef cattle farms in southern Xinjiang[J].China Animal Health Inspection,2022,39(7):22-28.(in Chinese)
[4] BLAKEBROUGH-HALL C,HICK P,MAHONY T J,et al.Factors associated with Bovine Respiratory Disease case fatality in feedlot cattle[J].J Anim Sci,2022,100(1):skab361.
[5] RAHE M C,MAGSTADT D R,GROELTZ-THRUSH J,et al.Bovine coronavirus in the lower respiratory tract of cattle with respiratory disease[J].J Vet Diagn Invest,2022,34(3):482-488.
[6] SOULES K R,RAHE M C,PURTLE L,et al.Bovine coronavirus infects the respiratory tract of cattle challenged intranasally[J].Front Vet Sci,2022,9:878240.
[7] FULTON R W,STEP D L,WAHRMUND J,et al.Bovine coronavirus (BCV) infections in transported commingled beef cattle and sole-source ranch calves[J].Can J Vet Res,2011,75(3):191-199.
[8] VLASOVA A N,SAIF L J.Bovine coronavirus and the associated diseases[J].Front Vet Sci,2021,8:643220.
[9] OMA V S,TRÅVÉN M,ALENIUS S,et al.Bovine coronavirus in naturally and experimentally exposed calves; viral shedding and the potential for transmission[J].Virol J,2016,13:100.
[10] ROJAS M,MANCHEGO A,ROCHA C B,et al.Outbreak of diarrhea among preweaning alpacas (Vicugna pacos) in the southern Peruvian highland[J].J Infect Dev Ctries,2016,10(3):269-274.
[11] WOO P C Y,LAU S K P,FAN R Y Y,et al.Isolation and characterization of dromedary camel coronavirus UAE-HKU23 from dromedaries of the middle east:minimal serological cross-reactivity between MERS coronavirus and dromedary camel coronavirus UAE-HKU23[J].Int J Mol Sci,2016,17(5):691.
[12] ELAZHARY M A S Y,FRECHETTE J L,SILIM A,et al.Serological evidence of some bovine viruses in the caribou (Rangifer tarandus caribou) in Quebec[J].J Wildl Dis,1981,17(4):609-612.
[13] YANG D K,HWANG I J,KIM B H,et al.Serosurveillance of viral diseases in Korean native goats (Capra hircus)[J].J Vet Med Sci,2008,70(9):977-979.
[14] KIM E M,CHO H C,SHIN S U,et al.Prevalence and genetic characterization of bovine coronavirus identified from diarrheic pre-weaned native Korean calves from 2019 to 2021[J].Infect Genet Evol,2022,100:105263.
[15] CLARK M A.Bovine coronavirus[J].Br Vet J,1993,149(1):51-70.
[16] SUZUKI T,OTAKE Y,UCHIMOTO S,et al.Genomic characterization and phylogenetic classification of bovine coronaviruses through whole genome sequence analysis[J].Viruses,2020,12(2):183.
[17] ZHU Q H,LI B,SUN D B.Advances in bovine coronavirus epidemiology[J].Viruses,2022,14(5):1109.
[18] 杨海峰,粟灵琳,王婧,等.我国14省市呼吸道综合征患牛冠状病毒感染的检测[J].畜牧与兽医,2019,51(11):92-96.YANG H F,SU L L,WANG J,et al.Detection of bovine coronavirus infection in calves with respiratory disease syndrome in 14 provinces of China[J].Animal Husbandry & Veterinary Medicine,2019,51(11):92-96.(in Chinese)
[19] 叶树华.牛冠状病毒病的防治[J].畜牧兽医科技信息,2008(6):39.YE S H.Prevention and treatment of bovine coronavirus disease[J].Chinese Journal of Animal Husbandry and Veterinary Medicine,2008(6):39.(in Chinese)
[20] AZIZ S,ALMAJHDI F N,WAQAS M,et al.Contriving multi-epitope vaccine ensemble for monkeypox disease using an immunoinformatics approach[J].Front Immunol,2022,13:1004804.
[21] DECARO N,MARI V,DESARIO C,et al.Severe outbreak of bovine coronavirus infection in dairy cattle during the warmer season[J].Vet Microbiol,2008,126(1-3):30-39.
[22] LACHOWICZ-WOLAK A,KLIMOWICZ-BODYS M D,PȽONECZKA-JANECZKO K,et al.The prevalence,coexistence,and correlations between seven pathogens detected by a PCR method from south-western Poland dairy cattle suffering from bovine respiratory disease[J].Microorganisms,2022,10(8):1487.
[23] FRUCCHI A P S,AGNOL A M D,BRONKHORST D E,et al.Bovine coronavirus co-infection and molecular characterization in dairy calves with or without clinical respiratory disease[J].Front Vet Sci,2022,9:895492.
[24] ATASOY M O,ISIDAN H,TURAN T.Genetic diversity,frequency and concurrent infections of picobirnaviruses in diarrhoeic calves in Turkey[J].Trop Anim Health Prod,2022,54(2):127.
[25] ZHU Q H,SU M J,LI Z J,et al.Epidemiological survey and genetic diversity of bovine coronavirus in Northeast China[J].Virus Res,2022,308:198632.
[26] DUSE A,OHLSON A,STENGÄRDE L,et al.Associations between bovine coronavirus and bovine respiratory syncytial virus infections and productivity,health status and occurrence of antimicrobial resistance in Swedish dairy herds[J].Antibiotics (Basel),2021,10(6):641.
[27] 杨朝辉,马雪连,王凡,等.安格斯母牛初乳产量与质量测定及其新生犊牛被动免疫效果评价[J].中国畜牧兽医,2022,49(9):3353-3361.YANG C H,SU X L,WANG F,et al.Determination of colostrum yield and quality of angus cows and evaluation of passive immunity effect of newborn calves[J].China Animal Husbandry & Veterinary Medicine,2022,49(9):3353-3361.(in Chinese)
[28] GALAN B S M,DE CARVALHO F G,CARVALHO S C S,et al.Casein and whey protein in the breast milk ratio:could it promote protein metabolism enhancement in physically active adults?[J].Nutrients,2021,13(7):2153.
[29] WALDNER C L,ROSENGREN L B.Factors associated with serum immunoglobulin levels in beef calves from Alberta and Saskatchewan and association between passive transfer and health outcomes[J].Can Vet J,2009,50(3):275-281.
[30] CUTTANCE E L,MASON W A,LAVEN R A,et al.The relationship between failure of passive transfer and mortality,farmer-recorded animal health events and body weights of calves from birth until 12 months of age on pasture-based,seasonal calving dairy farms in New Zealand[J].Vet J,2018,236:4-11.
[31] TAKAMURA K,MATSUMOTO Y,SHIMIZU Y.Field study of bovine coronavirus vaccine enriched with hemagglutinating antigen for winter dysentery in dairy cows[J].Can J Vet Res,2002,66(4):278-281.
[32] TEMIZKAN S S,ALKAN F.Bovine coronavirus infections in Turkey:molecular analysis of the full-length spike gene sequences of viruses from digestive and respiratory infections[J].Arch Virol,2021,166(9):2461-2468.
[33] DAVID D,STORM N,ILAN W,et al.Characterization of winter dysentery bovine coronavirus isolated from cattle in Israel[J].Viruses,2021,13(6):1070.

Molecular Epidemiological Investigation of Bovine Coronavirus in Calf Diarrhea in Main Cattle Producing Areas of Xinjiang

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