14株北京地区犬细小病毒分离毒株的VP2、NS1基因序列分析

doi:10.11843/j.issn.0366-6964.2021.028

Abstract

Abstract: Fourteen strains of canine parvovirus (CPV) were successfully isolated and identified from feces swabs of dogs which were suspected to be infected CPV in Beijing, and their complete NS1 and VP2 genes were sequenced and analyzed. Among the 14 strains identified, the results showed that 7 strains were New CPV-2a and 7 strains were of CPV-2c. Besides, some new amino acid mutation sites were identified (amino acids 19, 33, 293, 588, 624 and 656 of the NS1 and amino acids 13 and 574 of the VP2). Phylogenetic analysis based on the VP2 and NS1 genes indicated that most of the New CPV-2a and CPV-2c strains were closely related to the isolated strains in Nanning, Guangxi, and Changchun, Jilin, indicating that the isolated strains had the same origin as the isolated strains in Guangxi or Jilin. This study provides a useful reference for better epidemiological investigation of canine parvovirus, and also builds a foundation for the in-depth study of the molecular mechanism of canine parvovirus variation and transmission.

Key words: canine parvovirus, isolation and identification, VP2 gene, NS1 gene, evolutionary analysis

CLC Number:

S852.659.2

LI Shaohan, YOU Xinyue, FAN Junwen, XU Yi, HAO Yunfeng, CUI Shangjin, QIN Tong. Sequence Analysis of the Complete VP2 and NS1 Genes of 14 Canine Parvovirus Isolates in Beijing[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 262-267.

References 13

[1]	COTMORE S F, TATTERSALL P. The autonomously replicating parvoviruses of vertebrates[J]. Adv Virus Res, 1987, 33:91-174.
[2]	HUEFFER K, PARKER J S L, WEICHERT W S, et al. The natural host range shift and subsequent evolution of canine parvovirus resulted from virus-specific binding to the canine transferrin receptor[J]. J Virol, 2003, 77(3):1718-1726.
[3]	NELSON C D S, PALERMO L M, HAFENSTEIN S L, et al. Different mechanisms of antibody-mediated neutralization of parvoviruses revealed using the Fab fragments of monoclonal antibodies[J]. Virology, 2007, 361(2):283-293.
[4]	GILBERT L, VALILEHTO O, KIRJAVAINEN S, et al. Expression and subcellular targeting of canine parvovirus capsid proteins in baculovirus-transduced NLFK cells[J]. FEBS Lett, 2005, 579(2):385-392.
[5]	SAXENA L, KUMAR G R, SAXENA S, et al. Apoptosis induced by NS1 gene of Canine Parvovirus-2 is caspase dependent and p53 independent[J]. Virus Res, 2013, 173(2):426-430.
[6]	WANG H L, JIN H L, LI Q, et al. Isolation and sequence analysis of the complete NS1 and VP2 genes of canine parvovirus from domestic dogs in 2013 and 2014 in China[J]. Arch Virol, 2016, 161(2):385-393.
[7]	MARTELLA V, DECARO N, ELIA G, et al. Surveillance activity for canine parvovirus in Italy[J]. J Vet Med Ser B, 2005, 52(7-8):312-315.
[8]	OHSHIMA T, HISAKA M, KAWAKAMI K, et al. Chronological analysis of canine parvovirus type 2 isolates in Japan[J]. J Vet Med Sci, 2008, 70(8):769-775.
[9]	BUONAVOGLIA C, MARTELLA V, PRATELLI A, et al. Evidence for evolution of canine parvovirus type 2 in Italy[J]. J Gen Virol, 2001, 82(Pt 12):3021-3025.
[10]	BATTILANI M, MODUGNO F, MIRA F, et al. Molecular epidemiology of canine parvovirus type 2 in Italy from 1994 to 2017:recurrence of the CPV-2b variant[J]. BMC Vet Res, 2019, 15(1):393.
[11]	YI L, TONG M, CHENG Y, et al. Phylogenetic analysis of canine parvovirus VP2 gene in China[J]. Transbound Emerg Dis, 2016, 63(2):e262-e269.
[12]	PÉREZ R, CALLEROS L, MARANDINO A, et al. Phylogenetic and genome-wide deep-sequencing analyses of canine parvovirus reveal co-infection with field variants and emergence of a recent recombinant strain[J]. PLoS One, 2014, 9(11):e111779.
[13]	HORIUCHI M, GOTO H, ISHIGURO N, et al. Mapping of determinants of the host range for canine cells in the genome of canine parvovirus using canine parvovirus/mink enteritis virus chimeric viruses[J]. J Gen Virol, 1994, 75(Pt 6):1319-1328.

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Sequence Analysis of the Complete VP2 and NS1 Genes of 14 Canine Parvovirus Isolates in Beijing

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