不同生理阶段边鸡卵巢转录谱的构建及卵泡发育相关基因的分析

doi:10.11843/j.issn.0366-6964.2022.02.010

Abstract

Abstract: To dig out the genes related to the follicular development and reveal the genetic resorce for strong nesting, the transcriptome profiles of ovaries of Bian chickens at different physiological stages were analyzed. The transcriptome profiles of ovaries of Bian chickens at 70 d (stage of ovary development), 165 d (beginning of egg laying), 220 d (eggs laying at peak) and 330 d (nesting) were constructed, RSEM method was used to analyze the correlations of ovarian gene expression at different ages, basing on the differentially expressed genes (DEGs), GO and KEGG analysis, the candidate genes related to follicular development were screened and verified by quantitative real-time PCR. The analysis of transcriptome profiles showed that in total, there were 18 891 genes were identified among which 18 063 were known genes and 828 were unknown genes. Among the differentially expressed genes, 40 genes correlated with follicles development were screened, of which 35 genes were positively related to the number of developed follicle. The expression level of FDX1L in Bian chicken ovary at 70, 220, 165 and 330 d gradually decreased, and the expression level of NDRG4 in Bian chicken ovary at 70, 165, 220 and 330 d showed a decreasing trend with the increase of age; The expression levels of MC3R and PRLHR were higher in ovaries of Bian chicken at 70 and 330 d than those at 165 and 220 d; VIP was only expressed in Bian chicken ovary on 70, 165 and 220 d. The 11 randomly selected DEGs were verified by real-time fluorescent quantitative PCR that their relative expression were consistent with the transcriptional expression profile. Furthermore, GO function enrichment found that the DEGs were mainly concentrated in "biological processes", followed by "cell components", and finally "molecular functions"; KEGG analysis found that the DEGs are mainly concentrated in signal transduction, immune system and signal molecules, and interaction pathways. By analyzing gene expression profiles of Bian chicken ovary at different physiological stages, mining genes related to follicular development, and using GO function enrichment analysis and KEGG analysis, the functional classification and signaling pathway of differentially expressed genes was obtained. It provides the basis for revealing the molecular regulation of the related genes and their nesting properties of the Bian chicken follicle development.

Key words: Bian chicken, ovary, transcriptome, differentially expressed gene, follicular development, nesting

CLC Number:

S831.2

ZHANG Junzhen, LI Cai'e, LIU Bo, LI Jianhui, ZHANG Meng, LI Yali, CHANG Qiangqiang. Construction of Ovary Transcription Profile of Bian Chicken at Different Physiological Stages and Analysis of Genes Related to Follicular Development[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 423-435.

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Construction of Ovary Transcription Profile of Bian Chicken at Different Physiological Stages and Analysis of Genes Related to Follicular Development

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