两广小花猪FOXL2基因多态性及其与几个重要经济性状的关联分析

doi:10.11843/j.issn.0366-6964.2023.03.010

Abstract

Abstract: The purpose of this study was to explore the SNPs of FOXL2 gene and their effects on ovulation status and slaughtering traits, in order to provide theoretical basis for molecular breeding and genetic improvement of Liangguang Spotted pigs in the future. A total of 107 Liangguang Spotted pigs at the age of (365±25) days were taken as the research objects. The SNPs of FOXL2 were detected using genotyping by target sequencing (GBTS) technology, and association analysis and haplotype analysis were performed on ovulation status, teat numbers and slaughter traits. A total of 24 mutation sites were detected in the FOXL2 gene of Liangguang Spotted pigs. After eliminating SNPs with InDel mutation and the mutation frequency less than 1%, 12 SNPs were analyzed. Among them, 2 SNPs were located in the coding region and 10 SNPs were located in the non-coding region. The association analysis showed that the g.79706726 T>C, g.79706898 C>T, g.79707794 T>C, and g.79709005 T>C showed significant effects on ovulation status (P＜0.05), but had no significant effects on teat numbers trait (P >0.05). The 6 SNPs (g.79707040 A>G, g.79708079 T>C, g.79708152 G>T, g.79706726 T>C, g.79706898 C>T, and g.79707794 T>C) had significant effects on bone weight, lean meat rate, ham weight, and left carcass weight (P＜0.05), and 3 SNPs (g.79707040 A>G, g.79708079 T>C, g.79708152 G>T) displayed significant effects on leaf fat weight (P<0.05). The associa-tion analysis of haplotype combination found that the 3 SNPs g.79706726 T>C, g.79706898 C>T, and g.79707794 T>C were strongly linked (r²>0.9). The H1H2 haplotype combination (g.79706726 T>C and g.79706898 C>T) had significant effects on ovulation status, bone weight, lean meat rate, ham weight, and left carcass weight (P<0.05). The bone weight, ham weight, and left carcass traits of individuals with CCTT haplotype were significantly higher than those with TTCC haplotype (P<0.05), and the lean meat rate trait of individuals with CCTT haplotype was significantly lower than those wtih TTCC haplotype (P<0.05). Four SNPs of FOXL2 gene were significantly associated with ovulation status, which were g.79706726 T>C, g.79706898 C>T, g.79707794 T>C, and g.79709005 T>C. In addition, the SNPs of FOXL2 associated with pig slaughter traits were found in this study, indicating that FOXL2 was not only associated with ovarian phenotype and function, but also relevant for pig growth and development. This study provided an important reference for the selection and breeding of FOXL2 gene in Liangguang Spotted pigs, and also provided a theoretical basis for molecular marker-assisted breeding.

Key words: ovary, slaughtering traits, FOXL2, SNPs, haplotype

CLC Number:

S828.2

LIAO Weili, ZHANG Xiaoke, ZENG Jianhua, YANG Linfang, LI Shuo, HU Mengting, GUO Yixuan, CHEN Zanmou, ZHANG Hao, LI Jiaqi, YUAN Xiaolong. Associations between FOXL2 Polymorphism and Several Important Economic Traits in Liangguang Spotted Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 956-965.

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Associations between FOXL2 Polymorphism and Several Important Economic Traits in Liangguang Spotted Pigs

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