胎儿期与成年期蒙古马骨骼肌肌纤维类型转化研究

doi:10.11843/j.issn.0366-6964.2022.01.009

Abstract

Abstract: The study aimed to explore the mechanism of muscle fiber type difference between fetal and adult Mongolian horses. In this study, three 4-month-old fetuses (two females and one male) and three 5-year-old healthy adult female horses were selected as a whole with 4 body-distributed and representative muscle tissues (triceps brachii, splenius, longissimus dorsi and gluteus medius). Fetal Mongolian horse muscle fiber was one group, and adult Mongolian horse muscle fiber was one group, the experiment carried out 3 biological repetitions.Firstly, the skeletal muscle samples of Mongolian horses were observed by immunohistochemical staining. Then, the skeletal muscle samples were subjected to RNA-seq to construct the mRNA expression profiles of skeletal muscle fibers in different periods of fetal and adult Mongolian horses. Differentially expressed genes were screened for GO and KEGG functional enrichment analysis. Histological results showed that the proportion of fast muscle fibers in the fetal period was significantly higher than that in the adult period. RNA-seq was used to screen candidate genes that might affect the transformation related to muscle fiber types. The gene expression profiles related to muscle fiber types in fetal and adult Mongolian horses were compared. A total of 250 differentially expressed genes were screened out. There were 27 up-regulated genes in adulthood, including TNNT1, MYOZ2 and ATP2A2, and 17 up-regulated genes in the fetal period, including MYL1, TNNT3 and ENO3. ATP2A2 and TNNT1 were mainly related to fast and slow muscle fiber type transformation, and these differentially expressed genes were also closely related to skeletal muscle growth and development. The results showed that the proportion of fast muscle fibers in fetal Mongolian horses was significantly higher than that in adult Mongolian horses. ATP2A2, MYOZ2 were mainly expressed in muscle fibers of adult Mongolian horse, ENO3, TNNI2 were mainly expressed in muscle fibers of fetal Mongolian horse. Calcium and AMPK signaling pathways are related to muscle fiber type conversion.The results of this experiment are of great significance to the study of Mongolian horse muscle fiber type transformation.

Key words: Mongolian horse, transcriptome, muscle fiber type

CLC Number:

S821.2

DING Wenqi, BAO Tugeqin, REN Xiujuan, MONGKE Togtokh, LA Xima, JIA Zijie, AN Tana, TIEMUQIER Aertengqimike, HAN Haige, TAO Nala, DUGARJAVIIN Manglai, BAI Dongyi. Study on Muscle Fiber Type Transformation of Mongolian Horse during Fetal and Adult Period[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 88-99.

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Study on Muscle Fiber Type Transformation of Mongolian Horse during Fetal and Adult Period

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