双组分系统CpxR影响禽致病性大肠杆菌的耐药性、抗血清杀菌能力及毒力

doi:10.11843/j.issn.0366-6964.2021.04.020

Abstract

Abstract: CpxR is the response regulator of the Cpx two component system (TCS), which is involving in maintaining stability of membrane and virulence through regulating the transcriptional expression of target genes in bacteria. This study investigated the effects of TCS CpxR on the biological characteristics, serum bactericidal resistance and pathogenicity of avian pathogenic Escherichia coli (APEC). The cpxR gene mutant and complemented strains of APEC was constructed by Red homologous recombination system and complementation plasmid. Then, the growth curve, motility, biofilm formation, antibiotic sensitivity, serum bactericidal resistance, virulence among wild-type, cpxR mutant and complemented strains were compared and analyzed. There is no significant difference in growth rate and motility among wild-type, cpxR mutant and complemented strains. Moreover, CpxR did not affect the biofilm formation of APEC. However, the antibiotic sensitivity assays showed that cpxR mutant was less resistant to amikacin and kanamycin. The results indicated that of CpxR could enhance the survival ability of APEC in serum. Animal infection experiment showed that the median lethal dose (LD₅₀) of wild-type, cpxR mutant and complemented strains were 7.50×10⁵, 7.50×10⁶ and 1.33×10⁶ CFU respectively, indicating that cpxR mutant strain exhibited attenuated virulence in ducks. These data indicated that TCS CpxR contributes to the antibiotic resistance, serum bactericidal resistance and virulence of APEC, providing help for elucidating the environment adaptability, survival ability and pathogenic mechanism of APEC.

Key words: avian pathogenic Escherichia coli, CpxR, antibiotic resistance, serum bactericidal resistance, virulence

CLC Number:

S852.612

YI Zhengfei, ZHANG Yaodong, WANG Yao, ZHU Hong, TAO Chenglin, AFAYIBO Dossêh Jean Apôtre, LI Tao, QI Jingjing, TIAN Mingxing, DING Chan, YU Shengqing, WANG Shaohui. Two Component System CpxR Contributes to the Antibiotic Resistance, Serum Bactericidal Resistance and Virulence of Avian Pathogenic Escherichia coli[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1053-1060.

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Two Component System CpxR Contributes to the Antibiotic Resistance, Serum Bactericidal Resistance and Virulence of Avian Pathogenic Escherichia coli

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