中药成分辛弗林对胶体金免疫层析法检测β-受体激动剂的影响

doi:10.11843/j.issn.0366-6964.2021.012.034

Abstract

Abstract: This study was conducted to explore the reason why traditional Chinese medicine extractives and swine urines from pigs fed with some herbal were tested positive by CGIA. A LC-MS/MS method was established to determine synephrine in pigs' urine and traditional Chinese medicine extractives. Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium's extractives were determined, along with urine after herbal feed. In vitro mouse liver microsomes model was used to incubate the extractives of Citri Reticulatae Pericarpium Viride, Citri Reticulatae Pericarpium, Magnoliae Officinalis Cortex, and Chaenomelis Fructus. Synephrine was detected in pig urines after feeding with Citri Reticulatae Pericarpium and Citri Reticulatae Pericarpium Viride, and the concentration was 1.36 and 1.65 μg·mL^-1 respectively. The concentration of synephrine in Citri Reticulatae Pericarpium and Citri Reticulatae Pericarpium Viride's extractive was 132.6 and 312.7 μg·mL^-1 respectively. Extractives of Magnoliae Officinalis Cortex and Chaenomelis Fructus were tested gradually from positive to negative during 2-5 h incubation. Extractives of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium were tested positive during 0-6 h incubation. The experiment group of negative herbs were tested negative during incubation, and control group of synephrine matched were tested positive during 0-6 h incubation. Great dosing of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium in swine can cause false positive results of β-agonists'CGIA test in swine urine, and this phenomenon is related to synephrine. Synephrine is hard to be metabolized by mouse liver microsomes in vitro.

Key words: β-agonists, colloidal gold immunochromatographic assay, traditional Chinese medicine, false positive, synephrine

CLC Number:

S859.84

WANG Zilin, TANG Ziheng, LIN Zening, LIU Wenzi, LIU Rong, ZHANG Jiahui, SHEN Xiangguang. Effect of Traditional Chinese Medicine Compound Synephrine on the Detection of β-agonists Using Colloidal Gold Immunochromatographic Assay[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3675-3680.

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Effect of Traditional Chinese Medicine Compound Synephrine on the Detection of β-agonists Using Colloidal Gold Immunochromatographic Assay

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