鸭不同肠段内菌群结构分析与拟杆菌分布研究

doi:10.11843/j.issn.0366-6964.2020.12.010

Abstract

Abstract: This study aimed to analyze the microbiota composition and diversity and the distribution of Bacteroides in duodenal, jejunal, ileal and caecal contents in healthy ducks. The contents of duodenum, jejunum, ileum and cecum were collected aseptically from 20 healthy adult Gaoyou ducks (70-day-old, half male and female), and the bacterial DNA of intestinal contents were extracted. The IonS5TMXL platform was used for 16S rRNA high-throughput sequencing, the microbiota structure and abundance, as well as the distribution of Bacteroides were analyzed. The results showed that the microbiota abundance in duodenal and jejunal contents was significantly higher than that in ileal and caecal contents (P<0.05), the microbiota diversity in ileal contents was the lowest. The microbiota structure in duodenal and jejunal contents was similar, they were different from that in ileum, and especially cecum. The dominant phyla in the intestinal contents of healthy duck were Bacteroidetes, Firmicutes, Proteobacteria and Fusobacteria. The relative abundance of these phyla were different in different intestinal segment contents. The different microbial species were colonized in different intestinal segments, and the differential phyla in duodenum, jejunum, ileum and cecum were Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes, respectively. There were 28 kinds of Bacteroides species in duck intestine, among them, B. acidifaciens, B. barnesiae, B. caccae, B. caecicola, B. coprocola, B. sp and B. luti were significantly clustered in the cecum, and B. caecigallinarum, B. plebeius and B. barnesiae were dominantly colonized in duck cecum. The results indicate that intestinal space significantly affects the microbiota abundance and diversity, and different intestinal segments were colonized by differential microorganism, which is consistent with the intestinal segment niche and function. In the cecum, the dominant colonization of Bacteroides may be related to its physiological and biochemical functions.

Key words: 16S rRNA sequencing, microbiota, intestine, bacteroides, duck

CLC Number:

S852.6
S834

ZHU Chunhong, TAO Zhiyun, LIU Hongxiang, SONG Weitao, ZHANG Shuangjie, XU Wenjuan, YUAN Zhiwei, LI Huifang. Analysis of Microbiota Structure and Bacteroides Distribution in Different Intestinal Segments in Duck[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(12): 3001-3012.

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Analysis of Microbiota Structure and Bacteroides Distribution in Different Intestinal Segments in Duck

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