OPN5-TSH-DIO2/DIO3和VIP-PRL在短光照抑制公山麻鸭睾丸活性中的表达规律

doi:10.11843/j.issn.0366-6964.2023.05.021

Abstract

Abstract: The purpose of this study was to explore the expression and possible relationship of main related factors of OPN5-TSH-DIO2/DIO3 pathway and VIP-PRL pathway in the process of short light inhibiting on reproductive function of male Shanma duck. In this experiment, 80 healthy male Shanma ducks with similar body weight and normal breeding period (220 days old) were selected as subjects. The long light (18L:6D) of the normal breeding period was maintained 15 d before the experiment, from d16, the illumination was shortened by 2 h per day, and the illumination time was 8 h per day (8L:16D) until the end of the experiment (d45). Blood samples and tissue samples of hypothalamus, pituitary and testis were collected on d15, d21, d30 and d45, and testicular development and expression of related genes and proteins in hypothalamus, pituitary and testis were detected by HE staining, ELISA, qRT-PCR and Western blot techniques. The results showed that, compared with long light exposure, short light exposure resulted in testicular atrophy, a significant decrease in the number of spermatogenic cells and sperms(P<0.05), a significant decrease in blood LH, PRL and testosterone levels(P<0.05), a decrease in GnRH expression and an increase in GnIH expression in hypothalamus, but the difference was not significant(P>0.05). GnIHR showed an upward trend and FSH showed a downward trend in pituitary, but the difference was not significant(P>0.05). GnRHR increased significantly in the later stage of the experiment(P<0.05). The gene expression levels of LH in pituitary and FSHR, LHR in testis was decreased, but there was no significant difference(P>0.05). In OPN5-TSH-DIO2/DIO3 pathway, the expression gene levels of OPN5, DIO2, TSH-β and the expression protein levels of OPN5 was decreased significantly(P<0.05), the expression protein levels of DIO2 was decreased, but there was no significant difference(P>0.05). While the expression trend of DIO3 gene was opposite to that of DIO2. Shortening light exposure time led to the increase of gene(P>0.05) and protein(P<0.05) expression level of DIO3. In VIP-PRL pathway, the gene expression of VIP in hypothalamus and PRL in pituitary decreased significantly(P<0.05), while the gene expression of PRLR in pituitary increased significantly(P<0.05), and the gene expression of PRLR in testis decreased significantly(P<0.05). The results showed that both OPN5-TSH-DIO2/DIO3 pathway and VIP-PRL pathway were involved in the process of short light inhibiting on reproductive performance of male Shanma duck, which mediated the inhibitory effect of short light on reproductive axis. Compared with the light conditions needed in normal breeding period, short light could inhibiting the efficiency of two pathways and down-regulate the activity of reproductive axis, but the mutual regulation between the two pathways remains to be further studied.

Key words: Shanma duck, illumination, testis, OPN5, PRL

CLC Number:

S834.3

SUN Junfeng, PAN Jianqiu, ZHANG Zhuoshen, JIANG Danli, SHENG Xu, CHEN Rong, XU Danning, TIAN Yunbo, HUANG Yunmao. The Expression Patterns of OPN5-TSH-DIO2/DIO3 and VIP-PRL in the Process of Repressing the Testicular Function of Male Shanma Duck under Short-day Photoperiod[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2001-2012.

References 49

[1]	SHINOMIYA A,SHIMMURA T,NISHIWAKI-OHKAWA T,et al.Regulation of seasonal reproduction by hypothalamic activation of thyroid hormone[J]. Front Endocrinol,2014,5:12.
[2]	NAKAYAMA T,YOSHIMURA T.Seasonal rhythms:the role of thyrotropin and thyroid hormones[J]. Thyroid,2018,28(1):4-10.
[3]	刘宏祥,朱春红,宋卫涛,等.光照时长对鸭产蛋性能的影响[J].中国家禽,2020,42(3):114-116.LIU H X,ZHU C H,SONG W T,et al.Effects of illumination durations on laying performance of duck[J]. China Poultry,2020,42(3):114-116.(in Chinese)
[4]	江丹莉,潘建秋,杨博,等.不同光照时间对蛋鸭产蛋及生殖激素的影响[J].仲恺农业工程学院学报,2018,31(2):37-41.JIANG D L,PAN J Q,YANG B,et al.Effects of different lighting time on the egg production and reproductive hormone of duck[J]. Journal of Zhongkai University of Agriculture and Engineering,2018,31(2):37-41.(in Chinese)
[5]	LIUFU S,PAN J Q,SUN J F,et al.OPN5 regulating mechanism of follicle development through the TSH-DIO2/DIO3 pathway in mountain ducks under different photoperiods[J]. Front Physiol,2022,13:813881.
[6]	陈思颖,陈维虎,于静,等.光照对浙东白鹅产蛋及就巢相关行为的影响[J].中国畜牧杂志,2021,57(6):243-247.CHEN S Y,CHEN W H,YU J,et al.Effects of light on laying and nest-related behaviors of East Zhejiang white geese[J]. Chinese Journal of Animal Science,2021,57(6):243-247.(in Chinese)
[7]	ZHOU X L,JIANG D L,ZHANG Z S,et al.Expression of GnIH and its effects on follicle development and steroidogenesis in quail ovaries under different photoperiods[J]. Poult Sci,2022,101(12):102227.
[8]	黄江南,刘林秀,武艳平,等.光照时间对兴国灰鹅产蛋和PRL、FSHβ、LH mRNA表达的影响[J].江西农业大学学报,2015,37(2):308-313.HUANG J N,LIU L X,WU Y P,et al.Effects of light exposure on egg production and mRNA expression of PRL,FSHβ,LH of Xingguo gray geese[J]. Acta Agriculturae Universitatis Jiangxiensis,2015,37(2):308-313.(in Chinese)
[9]	PAN J Q,LIUFU S,SUN J F,et al.Long-day photoperiods affect expression of OPN5 and the TSH-DIO2/DIO3 pathway in Magang goose ganders[J]. Poult Sci,2022,101(10):102024.
[10]	ROSE E M,HAAKENSON C M,BALL G F.Sex differences in seasonal brain plasticity and the neuroendocrine regulation of vocal behavior in songbirds[J]. Horm Behav,2022,142:105160.
[11]	NAKAO N,ONO H,YAMAMURA T,et al.Thyrotrophin in the pars tuberalis triggers photoperiodic response[J]. Nature,2008,452(7185):317-322.
[12]	NAKANE Y,IKEGAMI K,ONO H,et al.A mammalian neural tissue opsin (Opsin 5) is a deep brain photoreceptor in birds[J]. Proc Natl Acad Sci U S A, 2010,107(34):15264-15268.
[13]	KANG S W,KUENZEL W J.Deep-brain photoreceptors (DBPs) involved in the photoperiodic gonadal response in an avian species, Gallus gallus[J]. Gen Comp Endocrinol,2015,211:106-113.
[14]	BEAUDRY F E G,IWANICKI T W,MARILUZ B R Z,et al.The non-visual opsins:eighteen in the ancestor of vertebrates, astonishing increase in ray-finned fish,and loss in amniotes[J]. J Exp Zool Part B Mol Dev Evol,2017,328(7):685-696.
[15]	HALFORD S,PIRES S S,TURTON M,et al.VA Opsin-based photoreceptors in the hypothalamus of Birds[J]. Curr Biol,2009, 19(16):1396-1402.
[16]	张磊,康波,姜冬梅,等.神经视蛋白的结构及其生物学功能研究进展[J].西北农林科技大学学报:自然科学版,2015, 43(8):46-50.ZHANG L,KANG B,JIANG D M,et al.Progress in structure and biological functions of neuropsin[J]. Journal of Northwest A&F University:Natural Science Edition,2015,43(8):46-50.(in Chinese)
[17]	YAMASHITA T.Unexpected molecular diversity of vertebrate nonvisual opsin Opn5[J]. Biophys Rev,2020,12(2):333-338.
[18]	BENOIT J.The role of the eye and of the hypothalamus in the photostimulation of gonads in the duck[J]. Ann N Y Acad Sci,1964, 117(1):204-215.
[19]	田继康,靳岚.血管活性肠肽的研究进展[J].药物生物技术,2013,20(6):560-563.TIAN J K,JIN L.Research progress in vasoactive intestinal peptide[J]. Pharmaceutical Biotechnology,2013,20(6):560-563.(in Chinese)
[20]	张耀,郭定宗.催乳素结构与功能研究进展[J].动物医学进展,2007,28(5):49-52.ZHANG Y,GUO D Z.Progress on structure and function of prolactin[J]. Progress in Veterinary Medicine,2007,28(5):49-52.(in Chinese)
[21]	LI X,JI W,SUN G,et al.Cloning and expression analysis of PRL and PRLR genes in black Muscovy duck[J]. Br Poult Sci,2020, 61(1):92-96.
[22]	GUMUȽKA M,HRABIA A,AVITAL-COHEN N,et al.The effect of parachlorophenylalanine treatment on the activity of gonadal and lactotrophic axes in native Polish crested chickens stimulated to broodiness[J]. Poult Sci,2020,99(5):2708-2717.
[23]	LEA R W,VOWLES D M.Vasoactive intestinal polypeptide stimulates prolactin release in vivo in the ring dove (Streptopelia risoria)[J]. Experientia,1986,42(4):420-422.
[24]	SHARP P J.Strategies in avian breeding cycles[J]. Anim Prod Sci,1996,42(1-4):505-513.
[25]	GENG A L,ZHANG J,ZHANG Y,et al.Effects of lighting regimes on performance,pineal melanopsin expression and melatonin content in native laying hens aged from 19 to 34 weeks[J]. Poult Sci,2022,101(1):101567.
[26]	CHEN R,GUO R H,ZHU H X,et al.Development of a sandwich ELISA for determining plasma prolactin concentration in domestic birds[J]. Domest Anim Endocrinol,2019,67:21-27.
[27]	苑志宇,董杨云逸,李锰,等.不同光照周期对鸡睾酮合成的影响[J].中国畜牧杂志,2019,55(3):99-102.YUAN Z Y,DONG Y Y Y,LI M,et al.Effects of different photoperiod on testosterone synthesis of roosters[J]. Chinese Journal of Animal Science,2019,55(3):99-102.(in Chinese)
[28]	KUENZEL W J,KANG S W,ZHOU Z J.Exploring avian deep-brain photoreceptors and their role in activating the neuroendocrine regulation of gonadal development[J]. Poult Sci,2015,94(4):786-798.
[29]	SHARP P J,BLACHE D.A neuroendocrine model for prolactin as the key mediator of seasonal breeding in birds under long-and short-day photoperiods[J]. Can J Physiol Pharmacol,2003,81(4):350-358.
[30]	ZHU H X,HU M D,GUO B B,et al.Effect and molecular regulatory mechanism of monochromatic light colors on the egg-laying performance of Yangzhou geese[J]. Anim Reprod Sci,2019,204:131-139.
[31]	黄运茂,施振旦,李孝伟,等.光照对马岗鹅季节性繁殖活动和内分泌的调控[J].畜牧兽医学报,2008,39(1):29-36.HUANG Y M,SHI Z D,LI X W,et al.Regulation of photoperiod on seasonal reproductive activities and endocrine in Magang Geese[J]. Acta Veterinaria et Zootechnica Sinica,2008,39(1):29-36.(in Chinese)
[32]	唐珊珊,卢光熹,陈元音,等.控制光照对非繁殖期种公鹅睾丸形态和组织学结构变化的研究[J].华南农业大学学报,1989, 10(1):59-64.TANG S S,LU G X,CHEN Y Y,et al.The effect of controlled light on the morphology and histological structure of the testes of stud Gandrs[J]. Journal of South China Agricultural University,1989,10(1):59-64.(in Chinese)
[33]	SINGH P,ANJUM S,SRIVASTAVA R K,et al.Central and peripheral neuropeptide RFRP-3:a bridge linking reproduction, nutrition, and stress response[J]. Front Neuroendocrinol,2022,65:100979.
[34]	CALLIGARO H, DKHISSI-BENYAHYA O, PANDA S.Ocular and extraocular roles of neuropsin in vertebrates[J]. Trends Neurosci, 2022, 45(3):200-211.
[35]	YOSHIMURA T,YASUO S,WATANABE M,et al.Light-induced hormone conversion of T4 to T3 regulates photoperiodic response of gonads in birds[J]. Nature,2003,426(6963):178-181.
[36]	MAJUMDAR G,RANI S,KUMAR V.Hypothalamic gene switches control transitions between seasonal life history states in a night-migratory photoperiodic songbird[J]. Mol Cell Endocrinol,2015,399:110-121.
[37]	ZHU H X,LIU X Q,HU M D,et al.Endocrine and molecular regulation mechanisms of the reproductive system of Hungarian White geese investigated under two artificial photoperiodic programs[J]. Theriogenology,2019,123:167-176.
[38]	NAKANE Y,YOSHIMURA T.Universality and diversity in the signal transduction pathway that regulates seasonal reproduction in vertebrates[J]. Front Neurosci,2014,8:115.
[39]	NAKANE Y,YOSHIMURA T.Deep brain photoreceptors and a seasonal signal transduction cascade in birds[J]. Cell Tissue Res, 2010, 342(3):341-344.
[40]	GOURDJI D,BATAILLE D,VAUCLIN N,et al.Vasoactive intestinal peptide (VIP) stimulates prolactin (PRL) release and cAMP production in a rat pituitary cell line (GH3/B6).Additive effects of VIP and TRH on PRL release[J]. FEBS Lett,1979,104(1):165-168.
[41]	OHTA H,KATO Y,SHIMATSU A,et al.Inhibition by antiserum to Vasoactive Intestinal Polypeptide (VIP) of prolactin secretion induced by serotonin in the rat[J]. Eur J Pharmacol,1985,109(3):409-412.
[42]	CICCONE N A,DUNN I C,BOSWELL T,et al.Gonadotrophin inhibitory hormone depresses gonadotrophin α and follicle-stimulating hormone β subunit expression in the pituitary of the domestic chicken[J]. J Neuroendocrinol,2004, 16(12):999-1006.
[43]	ROZENBOIM I,TABIBZADEH C,SILSBY J L,et al.Effect of ovine prolactin administration on hypothalamic vasoactive intestinal peptide (VIP),gonadotropin releasing hormone I and II content,and anterior pituitary VIP receptors in laying turkey hens[J]. Biol Reprod,1993,48(6):1246-1250.
[44]	CUI Y M,WANG J,ZHANG H J,et al.Effects of photoperiod on performance,ovarian morphology,reproductive hormone level,and hormone receptor mRNA expression in laying ducks[J]. Poult Sci,2021,100(4):100979.
[45]	TABIBZADEH C,ROZENBOIM I,SILSBY J L,et al.Modulation of ovarian cytochrome P45017α-hydroxylase and cytochrome aromatase messenger ribonucleic acid by prolactin in the domestic turkey[J]. Biol Reprod,1995,52(3):600-608.
[46]	CRISÓSTOMO S,GUÉMENÉ D,GARREAU-MILLS M,et al.Prevention of incubation behavior expression in Turkey hens by active immunization against prolactin[J]. Theriogenology,1998,50(4):675-690.
[47]	REDDY I J,DAVID C G,RAJU S S.Effect of suppression of plasma prolactin on luteinizing hormone concentration, intersequence pause days and egg production in domestic hen[J]. Domest Anim Endocrinol,2007,33(2):167-175.
[48]	GUÉMENÉ D,WILLIAMS J B.Relationships between broodiness expression laying persistency and concentrations of hormones during the first productive period in turkey hens (Meleagris gallopavo)[J]. Reprod Nutr Dev,1994,34(4):371-381.
[49]	ZHU H X,LIU X Q,CAI L P,et al.Cellular and molecular mechanisms of low dose prolactin potentiation of testicular development in cockerels[J]. Domest Anim Endocrinol,2019,69:51-61.

The Expression Patterns of OPN5-TSH-DIO2/DIO3 and VIP-PRL in the Process of Repressing the Testicular Function of Male Shanma Duck under Short-day Photoperiod

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