非洲猪瘟病毒MGF 360-9L基因序列分析、蛋白结构预测及亚细胞定位

doi:10.11843/j.issn.0366-6964.2020.020

Abstract

Abstract: Previous research results showed that African swine fever virus (ASFV) MGF360-9L can significantly inhibit the host's innate immune response. Herein we compared and analyzed MGF360-9L gene sequences of ASFV and predicted the relationship between MGF360-9L structure and function. In this study, bioinformatics method was used to analyze and predict its pri-mary, secondary and tertiary structure of the expressed protein. Based on the sequence of Georgia 2007/1 published in GenBank (FR682468.1), the MGF360-9L gene was synthesized and its recombinant eukaryotic expression plasmid was constructed. The recombinant plasmid was transfected into PK-15 cells, then gene expression was identified by Western blotting, and subcellular localization of this protein was observed by indirect immunoinfluscent assay (IFA). Results showed that MGF360-9L gene is highly conserved among type II ASFV strains, Georgia 2007/1 is the representative virus strain. The homology analysis results of 54 different ASFV strains is consistent with its phylogenetic relationships of them, the conserved sequence is a 378 bp fragment at the 3'terminal of MGF360-9L. The advanced structure of this protein was mainly α-helix. Nuclear location sequence (NLS) prediction revealed that it might be located in the nucleus, and is consistent with the results of MGF360-9L IFA in PK-15 cells. This study accumulated significant data for further research on inhibition of innate immune response by ASFV MGF360-9L and pathogenic mechanisms of the MGF360 gene family in ASFV.

Key words: African swine fever virus, MGF 360-9L gene, sequence analysis, structural prediction, subcellular localization

CLC Number:

S852.659.1

SHEN Chaochao, LI Guoli, ZHANG Dajun, XU Guowei, HOU Jing, LI Dan, DANG Wen, ZHANG Keshan, ZHENG Haixue, LIU Xiangtao. Gene Sequence Analysis, Protein Structure Prediction and Subcellular Localization of MGF 360-9L from African Swine Fever Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1371-1381.

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Gene Sequence Analysis, Protein Structure Prediction and Subcellular Localization of MGF 360-9L from African Swine Fever Virus

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