猪lncRNA TCONS_00791383对骨骼肌卫星细胞增殖分化的影响

doi:10.11843/j.issn.0366-6964.2020.002

Abstract

Abstract: The aim of this study was to investigate the effect of lncRNA TCONS_00791383 on the proliferation and differentiation of porcine skeletal muscle satellite cells. qRT-PCR was used to detect the expression levels of TCONS_00791383 in 6 tissues (heart, spleen, lung, kidney, dorsal muscle and leg muscles) of Yorkshire piglets within 7 days of birth; At the same time, the expression levels of TCONS_00791383 before and after proliferation and differentiation of porcine skeletal muscle satellite cells were examined. The expressions of the proliferation and differentiation marker genes were examined after knocking down TCONS_00791383 in porcine skeletal muscle satellite cells using antisense oligonucleotides(ASO) fragment. Target genes of TCONS_00791383 were predicted by using trans (co-expression) method, GO enrichment and KEGG pathway analysis of these target genes were performed by DAVID. The results showed that TCONS_00791383 had the highest expression level in heart, but no expression in spleen and kidney tissues. During the process of proliferation and differentiation of skeletal muscle satellite cells, the expression level of TCONS_00791383 gradually increased and reached the peak at 30 h of differentiation. Compared with the control group, after knocking down TCONS_00791383 using the ASO fragment, the expression levels of the proliferation marker genes Pax3 and Pax7 significantly or extremely significantly decreased (P<0.05,P<0.01), and the expression level of the differentiation marker gene MyoG extremely significantly decreased (P<0.01) at 24 h of differentiation, the expression level of the proliferation marker gene Pax3 extremely significantly decreased (P<0.01) and Pax7 gene significantly decreased (P<0.05), the expression level of the differentiation marker gene MyHC significantly decreased (P<0.05) at 48 h of differentiation. The predicted target genes were enriched in AMPK, ATP and other important signaling pathways involved in the proliferation and differentiation of skeletal muscle satellite cells. This study shows that lncRNA TCONS_00791383 probably promotes the proliferation and differentiation of porcine skeletal muscle satellite cells.

Key words: lncRNA TCONS_00791383, pig, skeletal muscle satellite cells, proliferation, differentiation

CLC Number:

S828.2

LI Qianqian, LI Long, HUANG Ziying, LI Changchun. Effect of Pig lncRNA TCONS_00791383 on the Proliferation and Differentiation of Skeletal Muscle Satellite Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1177-1186.

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Effect of Pig lncRNA TCONS_00791383 on the Proliferation and Differentiation of Skeletal Muscle Satellite Cells

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