鸡血糖性状的全基因组关联分析

doi:10.11843/j.issn.0366-6964.2020.003

Abstract

Abstract: This experiment aimed to explore the effective SNPs loci and functional genes related to blood glucose traits in chickens, and provide effective theoretical support for molecular breeding of high quality broilers. In this experiment, 407 Jing Xing Yellow hens were slaughtered at 98 days of age, blood DNA was extracted by phenol-chloroform method, and whole genome resequencing was performed at a depth of 10×; Glucose oxidase method was used to determine blood glucose levels in serum. The genome-wide association study (GWAS) were performed based on whole genome resequencing and glycemic phenotype data. GWAS screened a total of 6 blood glucose-related SNPs loci (associated threshold P<1.43×10^-6). The gene annotation revealed that rs734134177 was on the 8th intron of UBE3D gene, and its encoded protein was ubiquitin protein ligase. The blood glucose level of wild-type (AA) individuals at this locus was extremely significantly higher than that of mutant (GG) individuals (P<0.01); rs794554022 was extremely located at D 93.5 kb of ACAD9 gene downstream. ACAD9 protein was a member of the acyl-CoA dehydrogenase family and was the rate-limiting enzyme in the process of β-oxidation of fatty acyl-CoA in mitochondria. The blood glucose level of the wild-type(AA) individuals at the rs794554022 locus was extremely significantly lower than that of the mutant(CC) individuals (P<0.01). The above loci may be related candidate SNPs for regulating blood glucose level. The genes containing the two loci may be involved in the regulation of blood glucose metabolism in broilers. These results will provide candidate molecular markers for regulating the blood glucose metabolism of broilers and improving the meat quality, which will provide a new idea for the regulation of blood glucose metabolism in broilers.

Key words: blood glucose, chicken, whole genome resequencing, SNP, genome-wide association study (GWAS), molecular marker

CLC Number:

S831.2

LIU Xiaojing, LIU Lu, WANG Jie, CUI Huanxian, ZHAO Guiping, WEN Jie. Genome-wide Association Study of Chicken Blood Glucose Traits Using Whole Genome Resequencing[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1187-1195.

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Genome-wide Association Study of Chicken Blood Glucose Traits Using Whole Genome Resequencing

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