马立克病肿瘤组织内HSP60表达及生物学作用初步研究

doi:10.11843/j.issn.0366-6964.2019.12.017

Abstract

Abstract: This paper will focus on the relationship between HSP60 expression and the tumor induced by the Marek's disease(MD) virus. The animal tumor model was established using MD virus and chickens were timely killed post artificial infection. The relationship between pathological lesion and distribution of HSP60 were studied using histopathology and immunohistochemistry methods. Three small interfering RNA sequences of HSP60 were designed and recombinant lentivirus were constructed. Flow cytometry were used to study the effect of down-regulation of HSP60 to the apoptosis ratio of MSB-1 cells. Results showed that HSP60 was mainly located in the cytoplasm of tumor cells. Recombinant RNA interference lentiviruses targeting HSP60 were successfully constructed and the interference effect of 5147 sequence lentivirus was the best. At 48 h post transfection, the transcription, expression of HSP60 were significantly reduced (P<0.01), and the apoptosis ratio of MSB-1 cells was significantly induced (P<0.01) in lentivirus transfected group than those in control cell group and control sequence lentivirus transfected group. HSP60 is mainly expressed in the cytoplasm of tumor cells induced by the MDV and down-regulation of HSP60 is able to induce the apoptosis ratio of MSB-1 cells.

Key words: RNA interference, immunohistochemistry, heat shock protein 60, MSB-1 cells, apoptosis

CLC Number:

S852.3

WANG Junli, LI Yubao, DUAN Shihao, LU Jianbiao, DING Fei, SI Zhenshu, LI Xuyong, JIA Kejin, ZHANG Yunfei. Study on the Distribution of HSP60 in the MD Tumor Tissues and the Biological Function[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2529-2536.

References 27

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Study on the Distribution of HSP60 in the MD Tumor Tissues and the Biological Function

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