PSMD9对山羊前体脂肪细胞脂质沉积的调控作用研究

doi:10.11843/j.issn.0366-6964.2023.09.007

Abstract

Abstract: The study aimed to clone the PSMD9 gene sequence of goat and conduct the bioinformatics analysis. Then, the expression of PSMD9 in different tissues of goat and the expression level in intramuscular preadipocytes at different stages of differentiation was detected, and further revealed the effect of overexpression and interference of PSMD9 on lipid deposition in goat intramuscular preadipocytes. In this study, the sequence of PSMD9 gene was obtained by T-A cloning technique using 1-week-old healthy Jianzhou big-eared male goats (n=12), bioinformatics analysis was performed, and PSMD9 overexpression vector was constructed by double digestion method. PSMD9 was overexpressed in goat intramuscular adipocytes using liposome transfection, and the small interfering RNA (siRNA) interfered with PSMD9 expression. The effect of PSMD9 on lipid droplet formation was observed by Oil Red O staining and triglyceride content was measured by GPO-Trinder enzymatic reaction. The expression levels of PSMD9 in different tissues of goats and different stages of differentiation in intramuscular preadipocytes were measured by real-time quantitative PCR (RT-qPCR). The results showed that 763 bp nucleotide sequence of goat PSMD9 gene was obtained, including 5'UTR 67 bp, CDS 564 bp, and 3'UTR 132 bp, encoding 187 amino acid residues. The expression of PSMD9 gene in goat was highest in the liver and lowest in the spleen, and its expression level decreased gradually in goat precursor adipocytes in the former 4 days of differentiation induction, subsequently increased, and the expression level was the highest on the 8th day. Overexpression of the PSMD9 gene significantly increased the lipid droplets and triglyceride content in intramuscular preadipocytes and significantly up-regulated the relative expression of DGAT2, FASN and ACC, while the expression of DGAT1, PPARγ and SCD1 did not significantly changed. Interfering with PSMD9 gene decreased the lipid droplets and triglyceride content in intramuscular preadipocytes, while significantly down-regulated the expression of DGAT1, PPARγ, FASN and ACC, the expression of DGAT2 and SCD1 did not significantly changed. In this research, the CDS region sequence of PSMD9 gene was successfully obtained and its expression was highest in liver tissue. The expression of PSMD9 gene decreased gradually during differentiation in intramuscular adipocytes and reached the lowest level at day 4 in goat, after which the expression gradually increased.The expression of PSMD9 can significantly promote lipid deposition in goat's intramuscular preadipocytes. These results provide important data support for further elucidating the mechanism of PSMD9 gene regulating the formation of intramuscular fat in goat.

Key words: goat, PSMD9, RNA interference, overexpression, intramuscular precursor adipocytes

CLC Number:

S827.2

Cite this article

SHAO Peng, TANG Yinmei, LIN Yaqiu, WANG Yong, XIANG Hua, HUANG Lian, ZHU Jiangjiang. Regulation Effect of PSMD9 on Lipid Deposition in Goat Precursor Adipocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3653-3663.

share this article

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2023.09.007

https://www.xmsyxb.com/EN/Y2023/V54/I9/3653

References

[1] KUMAR P,SHARMA N,SHARMA S,et al.In-vitro meat:a promising solution for sustainability of meat sector[J].J Anim Sci Technol,2021,63(4):693-724.
[2] BAIK M,KANG H J,PARK S J,et al.TRIENNIAL GROWTH AND DEVELOPMENT SYMPOSIUM:Molecular mechanisms related to bovine intramuscular fat deposition in the longissimus muscle[J].J Anim Sci,2017,95(5):2284-2303.
[3] LIU K Q,YU W S,WEI W,et al.Melatonin reduces intramuscular fat deposition by promoting lipolysis and increasing mitochondrial function[J].J Lipid Res,2019,60(4):767-782.
[4] NISHIMURA T,HATTORI A,TAKAHASHI K.Structural changes in intramuscular connective tissue during the fattening of Japanese black cattle:effect of marbling on beef tenderization[J].J Anim Sci,1999,77(1):93-104.
[5] BAIK M,KANG H J,PARK S J,et al.TRIENNIAL GROWTH AND DEVELOPMENT SYMPOSIUM:Molecular mechanisms related to bovine intramuscular fat deposition in the longissimus muscle[J].J Anim Sci,2017,95(5):2284-2303.
[6] JEONG J,KWON E G,IM S K,et al.Expression of fat deposition and fat removal genes is associated with intramuscular fat content in longissimus dorsi muscle of Korean cattle steers[J].J Anim Sci,2012,90(6):2044-2053.
[7] 倪晓光,赵平.泛素-蛋白酶体途径的组成和功能[J].生理科学进展,2006,37(3):255-258.
NI X G,ZHAO P.The components and functions of the ubiquitin-proteasome pathway[J].Progress in Physiological Sciences,2006, 37(3):255-258.(in Chinese)
[8] XU G J,HUANG S,PENG J,et al.Targeting lipid metabolism in multiple myeloma cells:rational development of a synergistic strategy with proteasome inhibitors[J].Br J Pharmacol,2021,178(23):4741-4757.
[9] 贾彩凤.REGγ调控脂质代谢的研究[D].上海:华东师范大学,2014.
JIA C F.The essential role of REGγ in regulating lipid metabolism[D].Shanghai:East China Normal University,2014.(in Chinese)
[10] 王威.Rpn和Rac及脂质识别蛋白ML对褐飞虱发育、生殖的影响[D].杭州:浙江大学,2021.
WANG W.Effects of Rpn,Rac and lipid recognition protein ML on development and reproduction of brown planthopper (Nilaparvata lugens)[D].Hangzhou:Zhejiang University,2021.(in Chinese)
[11] WATANABE T K,SAITO A,SUZUKI M,et al.cDNA cloning and characterization of a human proteasomal modulator subunit, p27(PSMD9)[J].Genomics,1998,50(2):241-250.
[12] HOPPER J L,BEGUM N,SMITH L,et al.The role of PSMD9 in human disease:future clinical and therapeutic implications[J]. AIMS Mol Sci,2015,2(4):476-484.
[13] PARKER B L,CALKIN A C,SELDIN M M,et al.An integrative systems genetic analysis of mammalian lipid metabolism[J] Nature,2019,567(7747):187-193.
[14] GRAGNOLI C.PSMD9 gene in the NIDDM2 locus is linked to type 2 diabetes in Italians[J].J Cell Physiol,2010,222(2):265-267.
[15] GRAGNOLI C.Overweight condition and waist circumference and a candidate gene within the 12q24 locus[J].Cardiovasc Diabetol,2013,12:2.
[16] GRAGNOLI C,CRONSELL J.PSMD9 gene variants within NIDDM2 may rarely contribute to type 2 diabetes[J].J Cell Physiol, 2007,212(3):568-571.
[17] WILSON S G,ADAM G,LANGDOWN M,et al.Linkage and potential association of obesity-related phenotypes with two genes on chromosome 12q24 in a female dizygous twin cohort[J].Eur J Hum Genet,2006,14(3):340-348.
[18] ABERG K,DAI F,SUN G Y,et al.A genome-wide linkage scan identifies multiple chromosomal regions influencing serum lipid levels in the population on the Samoan islands[J].J Lipid Res,2008,49(10):2169-2178.
[19] THOMAS M K,YAO K M,TENSER M S,et al.Bridge-1,a novel PDZ-domain coactivator of E2A-mediated regulation of insulin gene transcription[J].Mol Cell Biol,1999,19(12):8492-8504.
[20] GRAGNOLI C.Hypercholesterolemia and a candidate gene within the 12q24 locus[J].Cardiovasc Diabetol,2011,10(1):38.
[21] KEATING M F,VALLIM T Q,PARKER B L,et al.PSMD9 identified as a novel regulator of hepatic acylglycerol metabolism using an integrated systems-biology approach[J].Obes Res Clin Pract,2019,13(3):272.
[22] 林森,林亚秋,朱江江,等.Wnt10b对山羊前体脂肪细胞分化相关基因表达的影响[J].畜牧兽医学报,2018,49(4):685-692.
LIN S,LIN Y Q,ZHU J J,et al.Effect of Wnt10b on the expression of precursor adipocytes differentiation related genes in goat[J].Acta Veterinaria et Zootechnica Sinica,2018,49(4):685-692.(in Chinese)
[23] 许晴,林森,朱江江,等.山羊肌内前体脂肪细胞诱导分化过程中内参基因的表达稳定性分析[J].畜牧兽医学报,2018, 49(5):907-918.
XU Q,LIN S,ZHU J J,et al.The expression stability analysis of reference genes in the process of goat intramuscular preadipocytes differentiation in goat[J].Acta Veterinaria et Zootechnica Sinica,2018,49(5):907-918.(in Chinese)
[24] OTTO T C,LANE M D.Adipose development:from stem cell to adipocyte[J].Crit Rev Biochem Mol Biol,2005,40(4):229-242.
[25] 杨阳.Zfp217参与转录调控和转录后m⁶A修饰调控脂肪细胞生成的机制[D].武汉:华中农业大学,2019.
YANG Y.Mechanism of Zfp217 regulating adipogenesis through transcriptional regulation and M⁶A posttranscriptional modification[D].Wuhan:Huazhong Agricultural University,2019.(in Chinese)
[26] 宋兴亚,彭巍,刘贤,等.m⁶A甲基化修饰及其影响动物脂肪生成的分子机制研究进展[J].中国畜牧杂志,2022,58(10):53-58.
SONG X Y,PENG W,LIU X,et al.The research progress in methylation modification of m⁶A and its molecular mechanism affecting the adipogenesis of animals[J].Chinese Journal of Animal Science,2022,58(10):53-58.(in Chinese)
[27] ARDITO F,GIULIANI M,PERRONE D,et al.The crucial role of protein phosphorylation in cell signaling and its use as targeted therapy (Review)[J].Int J Mol Med,2017,40(2):271-280.
[28] UHLEN M,OKSVOLD P,FAGERBERG L,et al.Towards a knowledge-based Human Protein Atlas[J].Nat Biotechnol,2010, 28(12):1248-1250.
[29] CALNAN D R,BRUNET A.The FoxO code[J].Oncogene,2008,27(16):2276-2288.
[30] 黄馨仪,郭飞,曾祥昌,等.短链脂肪酸作为信号分子在肠道炎症中的研究进展[J].中国临床药理学与治疗学,2019,24(11):1293-1299.
HUANG X Y,GUO F,ZENG X C,et al.Short-chain fatty acids as signal molecules in intestinal inflammation[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2019,24(11):1293-1299.(in Chinese)
[31] SIERSBÆK R,NIELSEN R,MANDRUP S.Transcriptional networks and chromatin remodeling controlling adipogenesis[J]. Trends Endocrinol Metab,2012,23(2):56-64.
[32] 刘珂含,王永,李艳艳,等.SRSF10对山羊肌内前体脂肪细胞分化的影响[J].畜牧兽医学报,2022,53(6):1768-1778.
LIU K H,WANG Y,LI Y Y,et al.Effects of SRSF10 on the differentiation of intramuscular preadipocytes in goats[J].Acta Veterinaria et Zootechnica Sinica,2022,53(6):1768-1778.(in Chinese)
[33] YANG C H,LI Q,LIN Y Q,et al.Diacylglycerol acyltransferase 2 promotes the adipogenesis of intramuscular preadipocytes in goat[J].Anim Biotechnol,2022,24:1-8.
[34] POL A,GROSS S P,PARTON R G.Biogenesis of the multifunctional lipid droplet:lipids,proteins,and sites[J].J Cell Biol,2014, 204(5):635-646.
[35] 杨昌恒,李琪,黄维,等.山羊DGAT1基因的克隆及对前体脂肪细胞脂质沉积的调控作用研究[J].畜牧兽医学报,2022, 53(1):76-87.
YANG C H,LI Q,HUANG W,et al.Cloning of goat DGAT1 gene and its regulation on lipid deposition in preadipocytes[J].Acta Veterinaria et Zootechnica Sinica,2022,53(1):76-87.(in Chinese)
[36] LI S,WU T,LU Y X,et al.Obesity promotes gastric cancer metastasis via diacylglycerol acyltransferase 2-dependent lipid droplets accumulation and redox homeostasis[J].Redox Biol,2020,36:101596.
[37] 王芳,马红,张冬杰,等.猪FASN基因功能性单核苷酸多态性的生物信息学分析[J].黑龙江畜牧兽医,2023(11):62-66,137.
WANG F,MA H,ZHANG D J,et al.Bioinformatics analysis of functional SNPs in pig FASN genes[J].Heilongjiang Animal Science and Veterinary Medicine,2023(11):62-66,137.(in Chinese)
[38] XU H F,LUO J,ZHAO W S,et al.Overexpression of SREBP1(sterol regulatory element binding protein 1) promotes de novo fatty acid synthesis and triacylglycerol accumulation in goat mammary epithelial cells[J].J Dairy Sci,2016,99(1):783-795.
[39] DANDAN M,HAN J L,MANN S,et al.Acetyl-CoA carboxylase inhibitor increases LDL-apoB production rate in NASH with cirrhosis:prevention by fenofibrate[J].J Lipid Res,2023,64(3):100339.

Related Articles 15

[1] YAN Xiaochun, XI Haijiao, LI Jinquan, WANG Zhiying, SU Rui. Study on Estimates of Genomic Breeding Value of Fleece Traits in Inner Mongolia Cashmere Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 120-128.

[2] ZHANG Chenjian, LI Yinxia, DING Qiang, LIU Weijia, WANG Huili, HE Nan, WU Jiashun, CAO Shaoxian. Efficient Preparation of CRISPR/Cas9-mediated Goat SOCS2 Gene Edited Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 129-141.

[3] ZHANG De'an, YANG Ruozhu, LIU Jie, LIU Dewu, DENG Ming, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Expression Analysis of Transcriptome in the Liver of Chuanzhong Black Goats Fed with Silage Neolamarckia Cadamba Substitute for Silage Corn [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 232-244.

[4] TANG Yinmei, LI Qi, LI Haiyang, LIN Yaqiu, WANG Yong, XIANG Hua, HUANG Lian, ZHU Jiangjiang. Cloning of the Goat FATP2 Gene and Its Regulatory Effect on Lipid Deposition in Precursor Adipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3642-3652.

[5] LIANG Rui, FAN Xiaorui, ZHANG Jinqiang, PANG Quanhai. Effects of Mouse Melanocyte Silencing and Overexpression of Pigment Epithelium-Derived Factor on Melanin Synthesis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3916-3930.

[6] WANG Wanjie, CHEN Nanzhu, ZOU Huiying, ZHOU Xinyi, HAO Haisheng, PANG Yunwei, ZHU Huabin, ZHAO Xueming, YU Dawei, DU Weihua. Effects of Histone Methyltransferase ASH1L Overexpression on Proliferation and Apoptosis of Bovine Cumulus Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3358-3368.

[7] LIU Xinhuan, YUN Jialei, MAO Li, LI Jizong, HAO Fei, HE Miaofeng, YANG Leilei, ZHANG Wenwen, CHENG Zilong, SUN Min, LIU Maojun, WANG Shaohui, BAI Juan, LI Wenliang. Isolation, Identification, Virulence Genes and Drug Resistance Analysis of Escherichia coli Isolated from Diarrheal Goat and Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3445-3454.

[8] ZHANG Renbao, ZHOU Donghui, ZHOU Lisheng, GAO Xiaoxiao, LIU Nan, HE Jianning. Analysis of Genetic Structure of Conservation Population in Jining Gray Goats Based on 70 K SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2836-2847.

[9] SHAO Yuexin, ZHANG Xinyu, GE Liyan, SHI Huaiping. Cloning and RNA Interference Analysis of ATF4 Gene in Xinong Saanen Dairy Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2353-2364.

[10] LIU Jie, XU Xiangping, DENG Ming, ZOU Xian, JIANG Shengwei, LIU Dewu, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Effect of miR-144-5p Targeting WNT5a on the Proliferation and Apoptosis of Goat Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2421-2435.

[11] XU Tiantian, ZHANG Tongtong, WANG Meng, WANG Xin. Transcription Factor Foxq1 Affects the Proliferation of Hair Follicle Stem Cells in Cashmere Goats via WNT/β-catenin Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2653-2661.

[12] QUBI Wuqie, LI Yanyan, LI Xin, WANG Yong, WANG Youli, LIU Wei, ZHU Jiangjiang, LIN Yaqiu. APOA4 Gene Inhibits Intramuscular Adipocyte Differentiation in Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1927-1938.

[13] BO Luqi, WANG Xueying, HOU Jiaming, ZHANG Li, ZHANG Jianhui, RUAN Rulin, ZHANG Bohan, WANG Shuang, SONG Mingxin, ZHANG Ziqun. Interference of Ts-ODC Gene of Trichinella spiralis by siRNA-757 Effect on Its Acid Resistance [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2114-2125.

[14] ZHU Junru, WEI Wei, PEI Dangshuai, ZHANG Fenque, DUAN Yu, GUO Yongfeng, JIANG Yue, XIA Shuli, HAN Jing, HOU Jinxing, AN Xiaopeng. Effects of PDCD4 on the Apoptosis of Dairy Goat Mammary Epithelial Cells and the Synthesis of β-casein and TG [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1429-1440.

[15] CHEN Cancan, JIANG Jing, SUN Xiaoyan, REN Hangxing, LI Jie. Expression of AGRP Gene in Goat Tissue and Its Action Mechanism on Melanin Production [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1441-1451.

Recommended Articles

Metrics

Viewed

Full text

Abstract

Comments

Abstract

References

Related Articles

Recommended Articles

Metrics

Comments

TOP

Regulation Effect of PSMD9 on Lipid Deposition in Goat Precursor Adipocytes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YAN Xiaochun, XI Haijiao, LI Jinquan, WANG Zhiying, SU Rui. Study on Estimates of Genomic Breeding Value of Fleece Traits in Inner Mongolia Cashmere Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 120-128.
[2]	ZHANG Chenjian, LI Yinxia, DING Qiang, LIU Weijia, WANG Huili, HE Nan, WU Jiashun, CAO Shaoxian. Efficient Preparation of CRISPR/Cas9-mediated Goat SOCS2 Gene Edited Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 129-141.
[3]	ZHANG De'an, YANG Ruozhu, LIU Jie, LIU Dewu, DENG Ming, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Expression Analysis of Transcriptome in the Liver of Chuanzhong Black Goats Fed with Silage Neolamarckia Cadamba Substitute for Silage Corn [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 232-244.
[4]	TANG Yinmei, LI Qi, LI Haiyang, LIN Yaqiu, WANG Yong, XIANG Hua, HUANG Lian, ZHU Jiangjiang. Cloning of the Goat FATP2 Gene and Its Regulatory Effect on Lipid Deposition in Precursor Adipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3642-3652.
[5]	LIANG Rui, FAN Xiaorui, ZHANG Jinqiang, PANG Quanhai. Effects of Mouse Melanocyte Silencing and Overexpression of Pigment Epithelium-Derived Factor on Melanin Synthesis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3916-3930.
[6]	WANG Wanjie, CHEN Nanzhu, ZOU Huiying, ZHOU Xinyi, HAO Haisheng, PANG Yunwei, ZHU Huabin, ZHAO Xueming, YU Dawei, DU Weihua. Effects of Histone Methyltransferase ASH1L Overexpression on Proliferation and Apoptosis of Bovine Cumulus Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3358-3368.
[7]	LIU Xinhuan, YUN Jialei, MAO Li, LI Jizong, HAO Fei, HE Miaofeng, YANG Leilei, ZHANG Wenwen, CHENG Zilong, SUN Min, LIU Maojun, WANG Shaohui, BAI Juan, LI Wenliang. Isolation, Identification, Virulence Genes and Drug Resistance Analysis of Escherichia coli Isolated from Diarrheal Goat and Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3445-3454.
[8]	ZHANG Renbao, ZHOU Donghui, ZHOU Lisheng, GAO Xiaoxiao, LIU Nan, HE Jianning. Analysis of Genetic Structure of Conservation Population in Jining Gray Goats Based on 70 K SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2836-2847.
[9]	SHAO Yuexin, ZHANG Xinyu, GE Liyan, SHI Huaiping. Cloning and RNA Interference Analysis of ATF4 Gene in Xinong Saanen Dairy Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2353-2364.
[10]	LIU Jie, XU Xiangping, DENG Ming, ZOU Xian, JIANG Shengwei, LIU Dewu, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Effect of miR-144-5p Targeting WNT5a on the Proliferation and Apoptosis of Goat Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2421-2435.
[11]	XU Tiantian, ZHANG Tongtong, WANG Meng, WANG Xin. Transcription Factor Foxq1 Affects the Proliferation of Hair Follicle Stem Cells in Cashmere Goats via WNT/β-catenin Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2653-2661.
[12]	QUBI Wuqie, LI Yanyan, LI Xin, WANG Yong, WANG Youli, LIU Wei, ZHU Jiangjiang, LIN Yaqiu. APOA4 Gene Inhibits Intramuscular Adipocyte Differentiation in Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1927-1938.
[13]	BO Luqi, WANG Xueying, HOU Jiaming, ZHANG Li, ZHANG Jianhui, RUAN Rulin, ZHANG Bohan, WANG Shuang, SONG Mingxin, ZHANG Ziqun. Interference of Ts-ODC Gene of Trichinella spiralis by siRNA-757 Effect on Its Acid Resistance [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2114-2125.
[14]	ZHU Junru, WEI Wei, PEI Dangshuai, ZHANG Fenque, DUAN Yu, GUO Yongfeng, JIANG Yue, XIA Shuli, HAN Jing, HOU Jinxing, AN Xiaopeng. Effects of PDCD4 on the Apoptosis of Dairy Goat Mammary Epithelial Cells and the Synthesis of β-casein and TG [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1429-1440.
[15]	CHEN Cancan, JIANG Jing, SUN Xiaoyan, REN Hangxing, LI Jie. Expression of AGRP Gene in Goat Tissue and Its Action Mechanism on Melanin Production [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1441-1451.