光照周期对褪黑激素受体在母兔下丘脑-垂体-卵巢轴中分布与表达的影响

doi:10.11843/j.issn.0366-6964.2019.12.016

Abstract

Abstract: The purpose of the study was to investigate the effect of photoperiods on the distribution and expression patterns of melatonin receptor in the "hypothalamic-pituitary-gonadal axis" of female rabbits, and to further analyze the mechanism of melatonin regulating estrus in female rabbits under different photoperiods. A total of 48 5-month-old nulliparous female New Zealand rabbits, were reared in artificial illumination. They were randomly assigned to 3 groups of 16 each. All groups were reared in 12-h L/12-h D photoperiod for the first 10 days, then were reared in long light group (LD, L:D=16:8), short light group (SD, L:D=8:16) or control group (L:D=12:12) for the last 6 days separately. Light intensity was 80 lx, the total trial period was 16 days. The hypothalamus, pituitary and ovary samples were collected after the test. The effects of photoperiod on the distribution and expression of melatonin receptor subtypes in hypothalamus, pituitary and ovaries of female rabbits were investigated by real-time PCR, Western blot and immunohistochemical staining. The results showed that the expression of MT1 mRNA in the SD group was 88.8% higher than that in the LD group (P<0.05), and 54.9% higher than that in the control group (P<0.05), but there was no significant difference between the LD group and the control group. The expression of MT2 mRNA was not significantly different between different photoperiod groups (P>0.05). Immunohistochemical staining showed that the number of MT1 positive cells in the periventricular nucleus of the hypothalamus in the LD group was significantly lower than that in the SD group (69.4%, P<0.05) and the control group (37.3%, P<0.05), and that of SD group was 104.8% higher (P<0.05) than the control group. Meanwhile, the number of MT1 positive cells in the paraventricular nucleus of the LD group was 52.9% lower than that in the SD group (P<0.05), and that of the control group was 55.8% lower than that of the SD group (P<0.05), while there was no difference between the LD group and the control group (P>0.05). In the pituitary, the expression of MT1 mRNA in the SD group was 164% higher than that in the LD group (P<0.05) and 49.5% higher than that in the control group (P<0.05), and the LD group was significantly lower than the control group by 43.5% (P<0.05), while there was no significant difference in MT2 mRNA expression between different photoperiods (P>0.05). The expression of MT1 mRNA in the ovary of the LD group was 33.3% lower than that of the control group (P<0.05) and 53.6% lower than that of the SD group (P<0.05), and there was no significant difference between the SD group and the control group (P>0.05). However, the relative mRNA expression of MT2 of the ovary in the SD group was significantly higher than that of the control group by 90.0% (P<0.05) and the LD group by 100% (P<0.05), and there was no difference between the LD group and the control group (P>0.05). Short light photoperiod increased the expression of melatonin receptor MT1 subtype in hypothalamus, pituitary gland and ovary, rather than the MT2 receptor. These results suggest that the regulation mechanism of photoperiod on estrus in female rabbits may be realized by MT1 receptor pathway.

Key words: photoperiod, rabbit, hypothalamus, pituitary, ovary, MT1, MT2

CLC Number:

S852.16

WANG Wenli, ZHANG Yuxian, YUAN Zhanhang, WANG Zixu, DONG Yulan, CHEN Yaoxing. Effect of Photoperiods on Distribution and Expression of Melatonin Receptors in Hypothalamus-Pituitary-Ovary Axis in Female Rabbits[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2518-2528.

References 38

[1]	SHIU S Y W, LI L, XU J N, et al. Melatonin-induced inhibition of proliferation and G1/S cell cycle transition delay of human choriocarcinoma JAr cells:possible involvement of MT2(MEL1B) receptor[J]. J Pineal Res, 1999, 27(3):183-192.
[2]	GOLDMAN B D. Mammalian photoperiodic system:formal properties and neuroendocrine mechanisms of photoperiodic time measurement[J]. J Biol Rhythms, 2001, 16(4):283-301.
[3]	DUBOCOVICH M L, RIVERA-BERMUDEZ M A, GERDIN M J, et al. Molecular pharmacology, regulation and function of mammalian melatonin receptors[J]. Front Biosci, 2003, 8:d1093-d1108.
[4]	WITT-ENDERBY P A, BENNETT J, JARZYNKA M J, et al. Melatonin receptors and their regulation:biochemical and structural mechanisms[J]. Life Sci, 2003, 72(20):2183-2198.
[5]	NOSJEAN O, FERRO M, COGÉ F, et al. Identification of the melatonin-binding site MT3 as the quinone reductase 2[J]. J Biol Chem, 2000, 275(40):31311-31317.
[6]	VINCENT L, COHEN W, DELAGRANGE P, et al. Molecular and cellular pharmacological properties of 5-methoxycarbonylamino-N-acetyltryptamine (MCA-NAT):a nonspecific MT3 ligand[J]. J Pineal Res, 2010, 48(3):222-229.
[7]	MARKOWSKA M, MROZKOWIAK A, SKWARLO-SONTA K. Influence of melatonin on chicken lymphocytes in vitro:involvement of membrane receptors[J]. Neuro Endocrinol Lett, 2002, 23(Suppl 1):67-72.
[8]	周瑞祥, 魏建恩,刘卉,等. 松果体及其褪黑素影响胸腺T细胞发育周期[J]. 解剖学报, 2005, 36(1):70-75.ZHOU R X, WEI J E, LIU H, et al. Pineal gland and melatonin influence the cell cycle of thymocyte[J]. Acta Anatomica Sinica, 2005, 36(1):70-75. (in Chinese)
[9]	CHEN F J, REHEMAN A, CAO J, et al. Effect of melatonin on monochromatic light-induced T-lymphocyte proliferation in the thymus of chickens[J]. J Photochem Photobiol B:Biol, 2016, 161:9-16.
[10]	裴小英, 郭定宗,郭锐. 松果体和褪黑素的生物学作用研究进展[J]. 中国畜牧兽医, 2007, 34(7):60-63.PEI X Y, GUO D Z, GUO R. Advances in the biological effects of pineal gland and melatonin[J]. China Animal Husbandry & Veterinary Medicine, 2007, 34(7):60-63. (in Chinese)
[11]	王淑娟, 刘文举,王立克,等. 褪黑激素对雌性动物生殖系统调节作用的研究进展[J]. 江苏农业科学, 2016, 44(6):15-20.WANG S J, LIU W J, WANG L K, et al. Research progress on the regulation of melatonin on female reproductive system[J]. Jiangsu Agricultural Sciences, 2016, 44(6):15-20. (in Chinese)
[12]	BALÍK A, KRETSCHMANNOVÁ K, MAZNA P, et al. Melatonin action in neonatal gonadotrophs[J]. Physiol Res, 2004, 53(S1):S153-S166.
[13]	TAMURA H, NAKAMURA Y, TERRON M P, et al. Melatonin and pregnancy in the human[J]. Reprod Toxicol, 2008, 25(3):291-303.
[14]	DUBOCOVICH M L, MARKOWSKA M. Functional MT1 and MT2 melatonin receptors in mammals[J]. Endocrine, 2005, 27(2):101-110.
[15]	FISCHER T W, SLOMINSKI A, ZMIJEWSKI M A, et al. Melatonin as a major skin protectant:from free radical scavenging to DNA damage repair[J]. Exp Dermatol, 2008, 17(9):713-730.
[16]	PANDI-PERUMAL S R, TRAKHT I, SRINIVASAN V, et al. Physiological effects of melatonin:role of melatonin receptors and signal transduction pathways[J]. Prog Neurobiol, 2008, 85(3):335-353.
[17]	SLOMINSKI A, FISCHER T W, ZMIJEWSKI M A, et al. On the role of melatonin in skin physiology and pathology[J]. Endocrine, 2005, 27(2):137-147.
[18]	SLOMINSKI A, TOBIN D J, ZMIJEWSKI M A, et al. Melatonin in the skin:synthesis, metabolism and functions[J]. Trends Endocrinol Metab, 2008, 19(1):17-24.
[19]	REPPERT S M, GODSON C, MAHLE C D, et al. Molecular characterization of a second melatonin receptor expressed in human retina and brain:the Mel1b melatonin receptor[J]. Proc Natl Acad Sci U S A, 1995, 92(19):8734-8738.
[20]	REPPERT S M, WEAVER D R, EBISAWA T, et al. Cloning of a melatonin-related receptor from human pituitary[J]. FEBS Lett, 1996, 386(2-3):219-224.
[21]	ROCA A L, GODSON C, WEAVER D R, et al. Structure, characterization, and expression of the gene encoding the mouse Mel1a melatonin receptor[J]. Endocrinology, 1996, 137(8):3469-3477.
[22]	DUBOCOVICH M L, HUDSON R L, SUMAYA I C, et al. Effect of MT1 melatonin receptor deletion on melatonin-mediated phase shift of circadian rhythms in the C57BL/6 mouse[J]. J Pineal Res, 2005, 39(2):113-120.
[23]	SÁNCHEZ-HIDALGO M, GUERRERO MONTÁV-EZ J M, CARRASCOSA-SALMORAL M D P, et al. Decreased MT1 and MT2 melatonin receptor expression in extrapineal tissues of the rat during physiological aging[J]. J Pineal Res, 2009, 46(1):29-35.
[24]	GUO Q Y, DONG Y L, CAO J, et al. Developmental changes of melatonin receptor expression in the spleen of the chicken, Gallus domesticus[J]. Acta Histochem, 2015, 117(6):559-565.
[25]	MONTGOMERY G W, MARTIN G B, PELLETIER J. Changes in pulsatile LH secretion after ovariectomy in Ile-de-France ewes in two seasons[J]. J Reprod Fertil, 1985, 73(1):173-183.
[26]	MASANA M I, DUBOCOVICH M L. Melatonin receptor signaling:finding the path through the dark[J]. Sci STKE, 2001, 2001(107):pe39.
[27]	张玉仙, 王文利,原展航,等. 不同光照周期对雌兔卵泡发育和下丘脑-垂体-卵巢轴的影响[J]. 畜牧兽医学报, 2019, 50(8):1694-1701.ZHANG Y X, WANG W L, YUAN Z H, et al. The effect of different photoperiods on follicular development and hypothalamus-pituitary-ovary axis in female rabbits[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(8):1694-1701. (in Chinese)
[28]	AGUILAR-ROBLERO R, GONZÁLEZ-MARISCAL G. Behavioral, neuroendocrine and physiological indicators of the circadian biology of male and female rabbits[J].Eur J Neurosci, 2018, doi:10. 1111/ejn. 14265.
[29]	BASURTO E, HOFFMAN K, LEMUS A C, et al. Electrolytic lesions to the anterior hypothalamus-preoptic area disrupt maternal nest-building in intact and ovariectomized, steroid-treated rabbits[J]. Horm Behav, 2018, 102:48-54.
[30]	KENNEDY G A, HUDSON R. Phase response curve to 1 h light pulses for the European rabbit (Oryctolagus cuniculus)[J]. Chronobiol Int, 2016, 33(8):1120-1128.
[31]	SPADONI G, BEDINI A, FURIASSI L, et al. Identification of bivalent ligands with melatonin receptor agonist and fatty acid amide hydrolase (FAAH) inhibitory activity that exhibit ocular hypotensive effect in the rabbit[J]. J Med Chem, 2018, 16(17):7902-7916.
[32]	王忻. 非繁殖季节光照控制对蒙古羊发情排卵和褪黑激素受体表达的影响[D]. 兰州:甘肃农业大学, 2009.WANG Q. Effects of light control on oestrus and ovulation and MTNR1a expression of Mongolia ewe in non-breeding season[D]. Lanzhou:Gansu Agricultural University, 2009. (in Chinese)
[33]	REPPERT S M, WEAVER D R, EBISAWA T. Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses[J]. Neuron, 1994, 13(5):1177-1185.
[34]	HOWARD C M, LUTTERSCHMIDT D I. The effects of melatonin on brain arginine vasotocin:relationship with sex and seasonal differences in melatonin receptor type 1 in green treefrogs (Hyla cinerea)[J]. J Neuroendocrinol, 2015, 27(8):670-679.
[35]	GAUER F, MASSON-PÉVET M, PÉVET P. Melatonin receptor density is regulated in rat pars tuberalis and suprachiasmatic nuclei by melatonin itself[J]. Brain Res, 1993, 602(1):153-156.
[36]	LINCOLN G A, ALMEIDA O F X, KLANDORF H, et al. Hourly fluctuations in the blood levels of melatonin, prolactin, luteinizing hormone, follicle-stimulating hormone, testosterone, tri-iodothyronine, thyroxine and cortisol in rams under artificial photoperiods, and the effects of cranial sympathectomy[J]. J Endocrinol, 1982, 92(2):237-250.
[37]	EL-RAEY M, GESHI M, SOMFAI T, et al. Evidence of melatonin synthesis in the cumulus oocyte complexes and its role in enhancing oocyte maturation in vitro in cattle[J]. Mol Reprod Dev, 2011, 78(4):250-262.
[38]	KANG J T, KOO O J, KWON D K, et al. Effects of melatonin on in vitro maturation of porcine oocyte and expression of melatonin receptor RNA in cumulus and granulosa cells[J]. J Pineal Res, 2009, 46(1):22-28.

Effect of Photoperiods on Distribution and Expression of Melatonin Receptors in Hypothalamus-Pituitary-Ovary Axis in Female Rabbits

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