Acta Veterinaria et Zootechnica Sinica ›› 2019, Vol. 50 ›› Issue (12): 2537-2544.doi: 10.11843/j.issn.0366-6964.2019.12.018

• CLINICAL VETERINARY MEDICINE • Previous Articles     Next Articles

Palmitic Acid Induced Steatosis in BRL 3A Cells by Up-regulating the Expression of ACC/FAS/DGAT2 Pathway

YANG Ling1,2,3, YANG Wenling1,2,3, ZHANG Huiyan1,2,3, BIAN Jianchun1,2,3, LIU Zongping1,2,3, YUAN Yan1,2,3, ZOU Hui1,2,3, GU Jianhong1,2,3*, LIU Xuezhong1,2,3*   

  1. 1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
    2. Jiangsu Co-innovation Center for Prevention and Contrlol of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China;
    3. Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China
  • Received:2019-07-17 Online:2019-12-23 Published:2019-12-20

PDF (PC)

148

Abstract: This paper will focus on the mechanism of palmitic acid-induced BRL 3A cell steatosis. BRL 3A cells were treated with different concentrations of PA (0, 0.2, 0.4, 0.6 mmol·L-1) for 24 h. The cell proliferation was detected by using CCK-8 method and RTCA technique, the cell lipid droplet formation was observed by using oil red O staining, the nucleus and cytoskeleton morphology were observed by using DAPI/F-actin double staining, the TG content was determined by colorimetry. The transcriptional levels of Acaca, Fasn and Dgat2 were detected by RT-PCR. The expressions of ACC, FAS, SCD1, GPAM and DGAT2 were detected by Western Blot. In result, compared with the control group, the 0.2, 0.4 mmol·L-1 PA group had no effect on cell proliferation and 0.6 mmol·L-1 PA inhibited cell proliferation. Oil red O staining showed that a large amount of lipid droplets accumulated in the 0.4 mmol·L-1 PA group, and obvious fatty degeneration occurred. With the increase of PA concentration, the nucleus shrinks, deforms and fragments; the cytoskeleton was destroyed and the microfilament breaks. With the increase of PA concentration, the TG content increased significantly (P<0.01). The transcriptional levels of Acaca, Fasn and Dgat2 mRNA in the lipid synthesis genes of different concentrations of PA (0.2, 0.4, 0.6 mmol·L-1) were significantly increased (P<0.05). The protein expression levels of ACC, FAS, SCD1, GPAM and DGAT2 were significantly increased (P<0.05). SCD1 protein expression was significantly lower in the 0.6 mmol·L-1 PA group compared with the 0.4 mmol·L-1 PA group (P<0.05). High concentration of palmitic acid inhibited the proliferation of BRL 3A cells and damaged the nucleus and skeleton. Palmitic acid induced the accumulation of lipid droplets and the increase of TG, and induced steatosis by up-regulating the transcription levels of Acaca, Fasn, Dgat2 mRNA and the expression of ACC/FAS/DGAT2 pathway proteins.

Key words: palmitic acid, steatosis, acetyl-CoA carboxylase, diacylglycerol acyltransferase 2, BRL 3A

CLC Number: