SRIF-14对绵羊子宫内膜上皮细胞增殖和凋亡的影响

doi:10.11843/j.issn.0366-6964.2023.08.018

Abstract

Abstract: The purpose of this study was to explore the role of somatostatin release inhibitory factor (SRIF) in sheep endometrial epithelial cells. The uterine tissue samples of sheep were took from the designated slaughterhouse of cattle and sheep in Shihezi city, Xinjiang. The expression of SRIF in the endometrium of sheep was determined by immunohistochemical staining. Estradiol (E₂), progesterone (P₄) and interferon-tau (IFNT) were used to simulate the hormone changes during embryo implantation in sheep endometrium. RT-qPCR and ELISA were used to detect the expression of progestogen and prostaglandins (PGs), and Western blot was used to detect the expression level of SRIF. SRIF-14 was added to sheep endometrial epithelial cells, and cell proliferation was detected by CCK-8 kit and RT-qPCR. The protein expression levels of Caspase 9, Bax, Bcl-2 and Cyt-c were detected by Western blot. The levels of PGE₂ and PGF_2α secreted by cells were detected by ELISA kit. Immunohistochemical staining results showed that SRIF was widely expressed in sheep endometrial tissues, and cell staining results confirmed that SRIF was expressed in sheep endometrial epithelial cells. The results of hormone combined treatment of sheep endometrial epithelial cells showed that the mRNA expression levels of ISG15, CXCL10, HOXA10 and RSAD2 were significantly increased, the secretion level of PGE₂ was increased, the secretion level of PGF_2α was decreased, and the protein expression level of SRIF was significantly increased after hormone treatment. The results of exogenous addition of SRIF-14 in sheep endometrial epithelial cells showed that SRIF-14 inhibited the proliferation of sheep endometrial epithelial cells and the expression level of CCNA mRNA was significantly decreased. The expression level of Bax, Caspase 9 and Cyt-c proteins were significantly increased, and the expression level of Bcl-2 protein was significantly decreased. The secretion level of PGE₂ increased and the secretion level of PGF_2α decreased. This study confirmed that SRIF is widely expressed in sheep endometrial tissues and epithelial cells. The addition of SRIF-14 to sheep endometrial epithelial cells can inhibit cell proliferation and induce apoptosis.

Key words: SRIF, sheep endometrial epithelial cells, proliferation, apoptosis

CLC Number:

S826.3

SONG Meijun, HAO Kexing, HAI Siyu, CHEN Yan, WANG Jing, HU Guangdong. Effects of SRIF-14 on Proliferation and Apoptosis of Endometrial Epithelial Cells in Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3325-3334.

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Effects of SRIF-14 on Proliferation and Apoptosis of Endometrial Epithelial Cells in Sheep

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