Ⅲ型分泌系统2转录因子YqeI对禽致病性大肠杆菌生物被膜调控机制的研究

doi:10.11843/j.issn.0366-6964.2019.12.013

Abstract

Abstract: Biofilm is one of the main cause of bacterial resistance. In this study, the effects of type Ⅲ secretion system 2 (ETT2) transcription regulator YqeI on the biofilm formation of avian pathogenic Escherichia coli and its influencing mechanism were investigated. Red homologous recombination method was used to construct yqeI gene deletion strains, and the effect of transcription regulator YqeI on the biofilm formation ability of avian pathogenic E. coli was explored by detecting the biofilm formation ability of wild strains and deletion strains, combining transcriptome sequencing and fluorescence quantitative detection of biofilm gene transription. The yqeI gene deletion strain was successfully constructed, and the deletion of yqeI does not affect the growth curve, but the biofilm formation ability was significantly decreased and the transription of the relevant biofilm gene was significantly down-regulated. The avian pathogenic E. coli ETT2 transcriptional regulator YqeI significantly affected the biofilm formation ability of avian pathogenic E.coli, providing a possibility to find new drug targets from the perspective of yqeI and related genes.

Key words: avian pathogenic Escherichia coli, type Ⅲ secretion system 2, transcription factor YqeI, biofilm

CLC Number:

S852.612

XIAO Yating, FU Dandan, MUHAMMAD AKMAL RAHEEM, XUE Mei, GU Yi, TU Jian, SONG Xiangjun, SHAO Ying, QI Kezong. Study on the Regulation Mechanism of Type Ⅲ Secretion System 2 Transcription Factor YqeI on the Biofilm of Avian Pathogenic Escherichia coli[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2488-2497.

References 32

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Study on the Regulation Mechanism of Type Ⅲ Secretion System 2 Transcription Factor YqeI on the Biofilm of Avian Pathogenic Escherichia coli

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