牛源金黄色葡萄球菌生物被膜与毒素基因检测及agr分型

doi:10.11843/j.issn.0366-6964.2020.11.029

Abstract

Abstract: The objective of the present study was to investigate the biofilm formation, antimicrobial resistance, virulence genes, and agr genotypes of Staphylococcus aureus (S. aureus) isolated from bovine mastitis cases, and to analyze the correlations between agr genotypes and virulence genes. Biofilm formation, antimicrobial resistance, and virulence genes for 336 strains of S. aureus were detected by microtiter plate method, disk diffusion method, and PCR respectively, and the agr typing of tested strains was determined by multiplex PCR. The results showed that all 336 strains of S. aureus from bovine mastitis were biofilm producers, among which 52.1% and 47.9% of isolates tested were moderate (++) and strong (+++) biofilm producers, respectively. Antimicrobial susceptibility testing demonstrated that S. aureus strains were highly resistant to penicillin, with a resistance rate of 91.7%, followed by erythromycin (89.6%), kanamycin (72.9%), clindamycin (66.7%) and gentamicin (60.4%). However, all isolates were sensitive to nitrofurantoin and linezolid. PCR results showed that the prevalence of fnbA gene was the highest (99.7%), followed by icaD (98.2%), icaA (89.6%), clfA (86.0%), cna (56.0%), and bap (14.6%) genes. Moreover, the sea, seb, sec and tst genes were found in 26.5%, 8.3%, 6.8% and 8.3% of the isolates, respectively. The agr typing results showed that S. aureus strains belonging to agr Ⅰ was predominant in our study, accounting for 77.1% of the isolates, and the frequencies of agr Ⅱ, agr Ⅲ and agr Ⅳ genotypes were 14.0%, 4.8% and 2.1%, respectively. Statistical analysis indicated that the strains of S. aureus belonging to agr Ⅰ genotype have the potential to carry more virulence genes, while no toxin genes could be found in any of the strains belonging to agr Ⅳ. The results revealed high antimicrobial resistance to common antimicrobial agents in S. aureus isolated from bovine mastitis milk samples. Moreover, agr Ⅰ was the predominant genotype with diverse toxin genes in S. aureus from bovine mastitis, and the potential hazard should be of concern.

Key words: bovine mastitis, Staphylococcus aureus, biofilm, virulence genes, agr typing

CLC Number:

S852.611

ZHANG Hang, LI Xinpu, WU Xiaohu, DING Xuezhi, YAN Zuoting, WANG Shengyi, LI Hongsheng. Investigation of Biofilm Formation, Virulence Genes and agr Typing of Staphylococcus aureus from Bovine Mastitis Cases[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2903-2910.

References 25

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Investigation of Biofilm Formation, Virulence Genes and agr Typing of Staphylococcus aureus from Bovine Mastitis Cases

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