禽致病性大肠杆菌clpV2基因缺失株的构建及生物学特性分析

doi:10.11843/j.issn.0366-6964.2021.011.032

Abstract

Abstract: This study was conducted to investigate the biological characteristics of the clpV2 gene in the type VI secretion system 2 (T6SS2) from avian pathogenic Escherichia coli (APEC). The clpV2 deleted strain was constructed by the Red recombination system. In addition, the clpV2 gene was cloned into the expression vectors of pBR322 to construct the complementary strain. The mutant strains were proved to be genetically stable. Comparison of biological characteristics was performed. The data demonstrated that, the mutant strains showed no differences in growth curve and sensitivity to multiple antibiotics. But compared with the wild type strain, the motility and biofilm formation of the deleted strain were decreased significantly. This work proved that the clpV2 gene had partial impact on the biological characteristics of TW-XM. Also, this work provided further basis to investigate the function of the clpV2 gene which might also help in understanding the pathogenesis of APEC.

Key words: avian pathogenic Escherichia coli, type VI secretion system, clpV2 gene, biological characteristics

CLC Number:

S852.61

ZHONG Haoran, WANG Peili, GUO Jia, WANG Heng, ZHU Guoqiang, LI Jianji, CUI Luying, DONG Junsheng, MENG Xia. Construction and Biological Characteristics Analysis of clpV2 Gene Mutant in Avian Pathogenic Escherichia coli[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3312-3316.

References 11

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Construction and Biological Characteristics Analysis of clpV2 Gene Mutant in Avian Pathogenic Escherichia coli

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