伪狂犬病病毒gE基因DNA疫苗在动物体内诱导的免疫应答及IL-2对其作用影响的研究

doi:10.11843/j.issn.0366-6964.2014.09.015

Abstract

Abstract:

To evaluate immune responses of gE DNA vaccine of pseudorabies virus (PRV) and the enhancement potency of porcine interleukin-2 for its immunogenicity，the recombinant plasmid of pcDNA3.1(+)-gE1 and pcDNA3.1(+)-gE2-IL-2 were constructed and transfected into Vero cells.The expression of gE and IL-2 gene were identified by indirect immunofluorescent assay，RT-PCR and Western blotting.The results showed that recombinant plasmids were transiently expressed in Vero cells and the protein expressed in Vero cells had good reactogenicity.Furthermore，animals were immunized by recombinant plasmids，empty vector，inactivated virus and normal saline.Indirect ELISA and determination of T lymphocyte subsets showed that the recombinant plasmids were able to induce efficient immune responses in inoculated animals.The IL-2 gene showed the significant enhancement effects on the immunogenecity of PRV gE DNA vaccine.The animals immunized with the pcDNA3.1(+)-gE2-IL-2 recombinant plasmid and pcDNA3.1(+)-gE1+ pcDNA3.1(+)-IL-2 co-immunize provided over 80% protection in mice and over 60% protection in piglets against wild type virus challenge.These results confirm that the fusion protein and hybrid protein enhances the protection against PRV through both humoral and cell-mediated immunity.

CLC Number:

WANG Hong-guang，HAO Fei，TANG De-yuan，ZHANG Yuan-xin，MA Ping，LUO Xian-feng，ZENG Zhi-yong，LIU Xia，LIU Jian，LI Da. Immune Responses of Pseudorabies Virus gE DNA Vaccine in Animals and the Immune Effects of Interleukin-2 Gene on It[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1488-1496.

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Immune Responses of Pseudorabies Virus gE DNA Vaccine in Animals and the Immune Effects of Interleukin-2 Gene on It

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