牛超数排卵重组促卵泡素研究进展及其应用

doi:10.11843/j.issn.0366-6964.2025.05.006

摘要/Abstract

摘要：

超数排卵作为牛体内胚胎生产中的关键技术，不仅是获得胚胎的重要方法，也是其他胚胎工程技术的基础。在超数排卵生产体内胚胎的过程中，多种生殖激素被用来促进卵泡的发育和排卵，其中促卵泡素是至关重要且核心的激素之一。近年来，由于垂体提取促卵泡素产量的下降，市场对促卵泡素的需求却不断增加，这引发了越来越多研究者对重组类促卵泡素开发的研究以及其在牛超数排卵中效果的关注。本文基于促卵泡素蛋白结构以及蛋白改造工程相关理论基础，介绍了重组促卵泡的发展历程，同时综述了国内外近十年来重组促卵泡素应用于牛超数排卵相关的研究进展，还对其在牛超数排卵领域中的潜在关注点进行了深入思考。旨在为建立高效的重组促卵泡素超数排方案提供有价值的参考。

关键词: 牛, 超数排卵, 重组促卵泡素, 胚胎工程

Abstract:

Superovulation serves as a pivotal technology in bovine embryo in vivo production, constituting a vital method for embryo in vivo procurement and forming the foundation for various other embryonic engineering techniques. During the process of superovulation, a plethora of reproductive hormones are employed to foster follicular development and ovulation, among which follicle-stimulating hormone (FSH) is one of the most crucial and central hormones. In recent years, there has been a decline in the production of pituitary-derived FSH while the market demand for FSH continues to escalate. This discrepancy has prompted an increasing number of researchers to focus on the development of recombinant FSH and its efficacy in bovine superovulation. This review is grounded in the theoretical foundations of FSH protein structure and protein engineering, presenting the evolution of recombinant FSH and summarizing the research advancements concerning the application of recombinant FSH in bovine superovulation over the past decade, both domestically and internationally. Furthermore, it delves into potential areas of interest within the field of bovine superovulation. The aim is to provide valuable insights for the establishment of an efficient superovulation protocol utilizing recombinant FSH.

Key words: bovine, superovulation, recombinant FSH, embryo engineering

中图分类号:

S823.3

熊铿, 范浩杰, 王杰, 赵善江, 朱庆利, 胡智辉, 罗昊澍, 朱化彬. 牛超数排卵重组促卵泡素研究进展及其应用[J]. 畜牧兽医学报, 2025, 56(5): 2047-2055.

XIONG Keng, FAN Haojie, WANG Jie, ZHAO Shanjiang, ZHU Qingli, HU Zhihui, LUO Haoshu, ZHU Huabin. Recent Advances and Applications of Recombinant Follicle-Stimulating Hormone in Bovine Superovulation[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(5): 2047-2055.

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品种 Breed	FSH种类 Type of FSH	超排用量 Dosage of superovulation	头均可用胚胎数/枚 Number of available embryos per donor
中国荷斯坦牛Chinese Holstein	中科院垂体FSH	8~10 mg	6.36^[25]
文山牛Wenshan cattle	日本产FSH	22 AU	7.83^[26]
澳洲和牛Australian Wagyu	Folltropin-V	240 mg	10.2^[28]
和牛Wagyu	Folltropin-V	120 mg	6.10^[29]
比利时蓝白花牛Belgian Blue	Folltropin-V	700 IU	5.50^[30]
南阳牛Nanyang cattle	Folltropin-V	340 mg	3.32^[31]
西门塔尔牛Simmental	宁波第二激素厂FSH	150 IU	4.94^[32]
南阳牛Nanyang cattle	Folltropin-V	未给出	7.71^[33]
复州牛Fuzhou cattle	中科院垂体FSH	8.8 mg	7.38^[35]
复州牛Fuzhou cattle	日本产FSH	24 AU	7.50^[35]

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