基于16S rRNA测序分析敲除基因ZBED6后巴马猪肠道微生物菌群的变化

doi:10.11843/j.issn.0366-6964.2024.10.006

摘要/Abstract

摘要：

旨在探究在巴马猪中ZBED6基因敲除之后肠道菌群的变化和肌肉表型的关联。本研究以5月龄野生型和ZBED6敲除型广西巴马小型猪为研究对象，按性别和基因型分成3组进行比较，分别是野生型公猪比野生型母猪(公WT: 母WT)、野生型母猪比敲除型母猪(母WT: 母KO)、野生型公猪比敲除型公猪(公WT: 公KO)，利用16S rRNA基因的高通量测序技术分析各肠段微生物组成。结果表明：1)在巴马猪中，大肠的菌群多样性显著高于小肠；2)与雌性野生型猪相比，雄性野生型猪瘦肉率更高，回肠苏黎世杆菌属、盲肠链球菌属、直肠消化链球菌属在雄性野生型猪中显著富集，回肠乳杆菌属在雌性野生型猪中显著富集；3)敲除ZBED6之后，猪的肌肉生长增加。肠道菌群组成显示，母猪的回肠中乳杆菌含量显著下降，盲肠中SMB53的含量显著上升。敲除ZBED6后，公猪直肠中普雷沃菌属和瘤胃球菌属的含量显著上升，盲肠中SMB53的含量显著上升。结果提示，苏黎世杆菌属、链球菌属、消化链球菌属和乳杆菌属在肠道内的差异可能会影响能量代谢从而促进雄性野生型猪的肌肉生长；敲除了ZBED6之后，猪的盲肠中SMB53丰度的显著增加可能是导致肌肉增多的一个因素。

关键词: 巴马猪, ZBED6, 肠道微生物, SMB53

Abstract:

The aim of this study was to investigate the association between changes in gut microbiota and muscle phenotype in ZBED6-knockout Bama pigs. In this study, 5-month-old wild-type and ZBED6-knockout Guangxi Bama mini-pigs were divided into 3 groups based on sex and genotypes: wild-type boars compared with wild-type sows (male WT: female WT), wild-type sows compared with knock-out sows (female WT: female Ko), and wild-type boars compared with knock-out boars (male WT: male Ko). The microbial composition of each intestinal segment was analyzed by high-throughput sequencing of 16S rRNA gene. The results showed that: 1) The community diversity of large intestine in Bama pigs was significantly higher than that of small intestine; 2) Compared with female wild-type pigs, male wild-type pigs have higher lean meat percentage. Turicibacter in ileum, Streptococcus in cecum and Peptostreptococcaceae in rectum were found to be concentrated in male wild-type pigs, while Lactobacillus in ileum was found to be concentrated in female wild-type pigs; 3) After knockout of ZBED6, muscle growth increased in pigs. The content of Lactobacillus in ileum of sows was significantly decreased and SMB53 in cecum was significantly increased. After knockout of ZBDE6, Prevotella and Ruminococcus increased significantly in boar rectum, and SMB53 increased significantly in cecum. The results suggest that the differences in abundance of Turicibacter, Streptococcus, Peptostreptococcaceae and Lactobacillus in the gut may affect energy metabolism and promote muscle growth in male wild-type pigs; After ZBED6 knockout, an increase abundance in SMB53 in the cecum of pigs may be a factor contributing to muscle gain.

Key words: Bama pig, ZBED6, gut microbes, SMB53

中图分类号:

S828.2

徐成, 田文杰, 马月辉, 王圣楠, 蒋琳, 王丹丹. 基于16S rRNA测序分析敲除基因ZBED6后巴马猪肠道微生物菌群的变化[J]. 畜牧兽医学报, 2024, 55(10): 4302-4310.

Cheng XU, Wenjie TIAN, Yuehui MA, Shengnan WANG, Lin JIANG, Dandan WANG. Changes in Gut Microbiota after ZBED6 Knockout were Analyzed Based on 16S rRNA Sequencing[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4302-4310.

图/表 5

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