甲烷马赛球菌DZ1对小鼠血清氧化三甲胺和炎症因子、肝脏抗氧化能力及盲肠微生物区系的影响

doi:10.11843/j.issn.0366-6964.2024.10.039

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (10): 4679-4689.doi: 10.11843/j.issn.0366-6964.2024.10.039

甲烷马赛球菌DZ1对小鼠血清氧化三甲胺和炎症因子、肝脏抗氧化能力及盲肠微生物区系的影响

占小秀¹^,²(), 刘鹏宇¹^,², 向小娥³, 毛胜勇¹^,², 金巍¹^,²^,*()

1. 南京农业大学反刍动物营养与饲料工程技术研究中心, 南京 210095
2. 南京农业大学消化道微生物研究室, 南京 210095
3. 南京农业大学动物科学类国家级实验教学中心, 南京 210095

收稿日期:2023-10-10 出版日期:2024-10-23 发布日期:2024-11-04
通讯作者: 金巍 E-mail:2021105048@stu.njau.edu.cn;jinwei@njau.edu.cn
作者简介:占小秀(1998-), 女, 安徽太湖人, 硕士生, 主要从事反刍动物营养与瘤胃微生物研究, E-mail: 2021105048@stu.njau.edu.cn
基金资助:
江苏省自然科学基金(BK20211217);国家自然科学基金(31872381)

Effects of Methanomassiliicoccus DZ1 on Serum Trimethylamine-N-oxide and Inflammatory Factors, Liver Antioxidant Capacity and Cecum Microbiota in Mice

Xiaoxiu ZHAN¹^,²(), Pengyu LIU¹^,², Xiao'e XIANG³, Shengyong MAO¹^,², Wei JIN¹^,²^,*()

1. Ruminant Nutrition and Feed Engineering Technology Research Center, Nanjing Agricultural University, Nanjing 210095, China
2. Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
3. National Experimental Teaching Center of Animal Science, Nanjing Agricultural University, Nanjing 210095, China

Received:2023-10-10 Online:2024-10-23 Published:2024-11-04
Contact: Wei JIN E-mail:2021105048@stu.njau.edu.cn;jinwei@njau.edu.cn

摘要/Abstract

摘要：

甲烷马赛球菌是哺乳动物消化道中的固有菌群，能够利用三甲胺(TMA)生成甲烷，然而其对宿主的作用机制尚不十分清楚。本文旨在利用小鼠模型研究灌胃甲烷马赛球菌菌株DZ1活菌对小鼠血清氧化三甲胺(TMAO)和炎症因子、肝抗氧化能力及盲肠微生物区系的影响。试验采用14只5周龄雄性C57BL/6J小鼠(体重18.4±1.1 g)，随机分为对照组(n=7)和处理组(n=7)，单笼饲养。处理组每天灌胃200 μL DZ1菌液(1.09×10⁹个细胞·mL^-1)，对照组每天灌胃200 μL无菌PBS溶液，试验期4周。结果显示，与对照组相比，处理组小鼠日增重和采食量没有显著变化(P>0.05)。处理组小鼠血清TMAO浓度和炎症因子水平显著降低(P < 0.05)。处理组小鼠肝中超氧化酶歧化酶(SOD)活性显著升高(P=0.035)，肝总抗氧化能力(T-AOC)显著提高(P=0.039)。盲肠细菌16S rRNA基因测序结果显示，处理组和对照组菌群结构没有显著差异(ANOSIM，P=0.161)。处理组中疣微菌门(Verrucomicrobia)的相对丰度有降低的趋势(P=0.064)，Lawsonibacter属、瘤胃球菌属(Ruminococcus)和阿克曼菌属(Akkermansia)等的相对丰度显著降低(P < 0.05)。综上，灌胃甲烷马赛球菌菌株DZ1未显著影响小鼠盲肠细菌区系结构，但降低了小鼠血清TMAO和炎症因子水平，提高了肝组织总抗氧化能力。

关键词: 氧化三甲胺(TMAO), 甲烷马赛球菌, 肠道微生物, 炎症因子, 抗氧化能力

Abstract:

Methanomassiliicoccales is an indigenous group of methanogens in the mammalian gut that can use trimethylamine (TMA) to produce methane, but the mechanisms of its action on the host remain unclear. The aim of this study was to investigate the effects of Methanomethylophilus sp. DZ1 on serum trimethylamine-N-oxide (TMAO) and inflammatory factors, liver antioxidant capacity, and cecum microbiota in mice. Fourteen five-week-old male B7/6J mice (body weight 18.4±1.1g) were randomly divided into a control group (n=7) and a treatment group (n=7), single cage feeding. Each mouse in the treatment group was given 200 μL DZ1 (1.09×10⁹ cells·mL^-1) daily, and each mouse in the control group was given 200 μL sterile PBS solution daily. The experiment lasted for 4 weeks. The results showed that the body weight and feed intake of mice in the treatment group had no significant changes (P>0.05). Serum TMAO concentration and inflammatory factor levels of mice in the treatment group were significantly decreased (P < 0.05). The activities of superoxidase dismutase (SOD) in the liver of the treatment group were significantly increased (P=0.035), and the total antioxidant capacity (T-AOC) of the liver was significantly increased (P=0.039). The results of 16S rRNA gene sequencing of cecal bacteria showed no significant difference in bacterial community structure between the treatment group and control group (ANOSIM, P=0.161). The relative abundance of Verrucomicrobia in the treatment group significantly decreased (P=0.064). The relative abundances of Lawsonibacter, Ruminococcus, and Akkermansia were significantly decreased (P < 0.05). In summary, the strain DZ1 had no significant effect on the bacterial community structure of the cecum in mice, but decreased the levels of serum TMAO and inflammatory factors, and increased the total antioxidant capacity of the liver.

Key words: trimethylamine-N-oxide(TMAO), Methanomassiliicoccus, gut microbiome, inflammatory factors, antioxidant capacity

中图分类号:

S852.6

占小秀, 刘鹏宇, 向小娥, 毛胜勇, 金巍. 甲烷马赛球菌DZ1对小鼠血清氧化三甲胺和炎症因子、肝脏抗氧化能力及盲肠微生物区系的影响[J]. 畜牧兽医学报, 2024, 55(10): 4679-4689.

Xiaoxiu ZHAN, Pengyu LIU, Xiao'e XIANG, Shengyong MAO, Wei JIN. Effects of Methanomassiliicoccus DZ1 on Serum Trimethylamine-N-oxide and Inflammatory Factors, Liver Antioxidant Capacity and Cecum Microbiota in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4679-4689.

图/表 11

表 1

表 2

图 1

图 2

图 3

图 4

表 3

表 4

图 5

表 5

表 6

参考文献 44

1	WANG Z N , ZHAO Y Z . Gut microbiota derived metabolites in cardiovascular health and disease[J]. Protein Cell, 2018, 9 (5): 416- 431. doi: 10.1007/s13238-018-0549-0
2	KOETH R A , WANG Z N , LEVISON B S , et al. Intestinal microbiota metabolism of L-carnitine, a nutrient in red meat, promotes atherosclerosis[J]. Nat Med, 2013, 19 (5): 576- 585. doi: 10.1038/nm.3145
3	CRACIUN S , MARKS J A , BALSKUS E P . Characterization of choline trimethylamine-lyase expands the chemistry of glycyl radical enzymes[J]. ACS Chem Biol, 2014, 9 (7): 1408- 1413. doi: 10.1021/cb500113p
4	HE M X , TAN C P , XU Y J , et al. Gut microbiota-derived trimethylamine-n-oxide: a bridge between dietary fatty acid and cardiovascular disease?[J]. Food Res Int, 2020, 138, 109812. doi: 10.1016/j.foodres.2020.109812
5	DOLPHIN C T , RILEY J H , SMITH R L , et al. Structural organization of the human flavin-containing monooxygenase 3 gene (FMO3), the favored candidate for fish-odor syndrome, determined directly from genomic DNA[J]. Genomics, 1997, 46 (2): 260- 267. doi: 10.1006/geno.1997.5031
6	MIAO J , LING A V , MANTHENA P V , et al. Flavin-containing monooxygenase 3 as a potential player in diabetes-associated atherosclerosis[J]. Nat Commun, 2015, 6, 6498. doi: 10.1038/ncomms7498
7	TANG W H W , WANG Z N , FAN Y Y , et al. Prognostic value of elevated levels of intestinal microbe-generated metabolite trimethylamine-N-oxide in patients with heart failure: refining the gut hypothesis[J]. J Am Coll Cardiol, 2014, 64 (18): 1908- 1914. doi: 10.1016/j.jacc.2014.02.617
8	ZHU W F , GREGORY J C , ORG E , et al. Gut microbial metabolite TMAO enhances platelet hyperreactivity and thrombosis risk[J]. Cell, 2016, 165 (1): 111- 124. doi: 10.1016/j.cell.2016.02.011
9	TANG W H W , WANG Z N , KENNEDY D J , et al. Gut microbiota-dependent trimethylamine N-oxide (TMAO) pathway contributes to both development of renal insufficiency and mortality risk in chronic kidney disease[J]. Cir Res, 2015, 116 (3): 448- 455. doi: 10.1161/CIRCRESAHA.116.305360
10	CHEN Y Y , WENG Z K , LIU Q , et al. FMO3 and its metabolite TMAO contribute to the formation of gallstones[J]. Biochim Biophys Acta Mol Basis Dis, 2019, 1865 (10): 2576- 2585. doi: 10.1016/j.bbadis.2019.06.016
11	SUN X L , JIAO X F , MA Y R , et al. Trimethylamine N-oxide induces inflammation and endothelial dysfunction in human umbilical vein endothelial cells via activating ROS-TXNIP-NLRP3 inflammasome[J]. Biochem Biophys Res Commun, 2016, 481 (1/2): 63- 70.
12	BORREL G , PARISOT N , HARRIS H M , et al. Comparative genomics highlights the unique biology of Methanomassiliicoccales, a thermoplasmatales-related seventh order of methanogenic archaea that encodes pyrrolysine[J]. BMC Genomics, 2014, 15, 679. doi: 10.1186/1471-2164-15-679
13	OREN A , GARRITY G M . List of new names and new combinations previously effectively, but not validly, published[J]. Int J Syst Evol Microbiol, 2013, 63 (9): 3131- 3134.
14	HENDERSON G , COX F , GANESH S , et al. Global Rumen Census Collaborators, et al.Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range[J]. Sci Rep, 2015, 5, 14567. doi: 10.1038/srep14567
15	ZHOU Y , JIN W , XIE F , et al. The role of methanomassiliicoccales in trimethylamine metabolism in the rumen of dairy cows[J]. Animal, 2021, 15 (7): 100259. doi: 10.1016/j.animal.2021.100259
16	REA S , BOWMAN J P , POPOVSKI S , et al. Methanobrevibacter millerae sp. nov. and Methanobrevibacter olleyae sp. nov., methanogens from the ovine and bovine rumen that can utilize formate for growth[J]. Int J Syst Evol Microbiol, 2007, 57 (3): 450- 456. doi: 10.1099/ijs.0.63984-0
17	BALCH W E , FOX G E , MAGRUM L J , et al. Methanogens: reevaluation of a unique biological group[J]. Microbiol Rev, 1979, 43 (2): 260- 296. doi: 10.1128/mr.43.2.260-296.1979
18	LOWE S E , THEODOROU M K , TRINCI A P J , et al. Growth of anaerobic rumen fungi on defined and semi-defined media lacking rumen fluid[J]. J Gen Microbiol, 1985, 131 (9): 2225- 2229.
19	HAI X , LANDERAS V , DOBRE M A , et al. Mechanism of prominent trimethylamine oxide (TMAO) accumulation in hemodialysis patients[J]. PLoS One, 2015, 10 (12): e0143731. doi: 10.1371/journal.pone.0143731
20	ZOETENDAL E G , AKKERMANS A D L , DE VOS W M . Temperature gradient gel electrophoresis analysis of 16s rRNA from human fecal samples reveals stable and host-specific communities of active bacteria[J]. Appl Environ Microbiol, 1998, 64 (10): 3854- 3859. doi: 10.1128/AEM.64.10.3854-3859.1998
21	JEYANATHAN J , KIRS M , RONIMUS R S , et al. Methanogen community structure in the rumens of farmed sheep, cattle and red deer fed different diets[J]. FEMS Microbiol Ecol, 2011, 76 (2): 311- 326. doi: 10.1111/j.1574-6941.2011.01056.x
22	MARTÍNEZ-DEL CAMPO A , BODEA S , HAMER H A , et al. Characterization and detection of a widely distributed gene cluster that predicts anaerobic choline utilization by human gut bacteria[J]. mBio, 2015, 6 (2): e00042- 15.
23	JOHNSON J S , SPAKOWICZ D J , HONG B Y , et al. Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis[J]. Nat Commun, 2019, 10 (1): 5029. doi: 10.1038/s41467-019-13036-1
24	SCHLOSS P D , WESTCOTT S L , RYABIN T , et al. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities[J]. Appl Environ Microbiol, 2009, 75 (23): 7537- 7541. doi: 10.1128/AEM.01541-09
25	LOZUPONE C , LLADSER M E , KNIGHTS D , et al. UniFrac: an effective distance metric for microbial community comparison[J]. ISME J, 2011, 5 (2): 169- 172. doi: 10.1038/ismej.2010.133
26	AMATO K R , YEOMAN C J , KENT A , et al. Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes[J]. ISME J, 2013, 7 (7): 1344- 1353. doi: 10.1038/ismej.2013.16
27	ALTAY O , NIELSEN J , UHLEN M , et al. Systems biology perspective for studying the gut microbiota in human physiology and liver diseases[J]. EBioMedicine, 2019, 49, 364- 373.
28	ARON-WISNEWSKY J , VIGLIOTTI C , WITJES J , et al. Gut microbiota and human NAFLD: disentangling microbial signatures from metabolic disorders[J]. Nat Rev Gastroenterol Hepatol, 2020, 17 (5): 279- 297.
29	秦士贞, 杨敏敏, 任志雄, 等. 枯草芽孢杆菌对脂多糖应激肉仔鸡肠道免疫、肠道组织形态以及肠道屏障的影响[J]. 畜牧兽医学报, 2023, 54 (11): 4676- 4690. doi: 10.11843/j.issn.0366-6964.2023.11.022
	QIN S Z , YANG M M , REN Z X , et al. Effects of Bacillus subtilis on intestinal immunity, intestinal tissue morphology and intestinal barrier of broilers challenged with lipopolysaccharide[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54 (11): 4676- 4690. doi: 10.11843/j.issn.0366-6964.2023.11.022
30	SAKAMOTO M , IINO T , YUKI M , et al. Lawsonibacter asaccharolyticus gen. nov., sp. nov., a butyrate-producing bacterium isolated from human faeces[J]. Int J Syst Evol Microbiol, 2018, 68 (6): 2074- 2081.
31	CHANG P V , HAO L M , OFFERMANNS S , et al. The microbial metabolite butyrate regulates intestinal macrophage function via histone deacetylase inhibition[J]. Proc Natl Acad Sci U S A, 2014, 111 (6): 2247- 2252.
32	BREBAN M , TAP J , LEBOIME A , et al. Faecal microbiota study reveals specific dysbiosis in spondyloarthritis[J]. Ann Rheum Dis, 2017, 76 (9): 1614- 1622.
33	DEL CHIERICO F , NOBILI V , VERNOCCHI P , et al. Gut microbiota profiling of pediatric nonalcoholic fatty liver disease and obese patients unveiled by an integrated meta-omics-based approach[J]. Hepatology, 2017, 65 (2): 451- 464.
34	CANI P D , DEPOMMIER C , DERRIEN M , et al. Akkermansia muciniphila: paradigm for next-generation beneficial microorganisms[J]. Nat Rev Gastroenterol Hepatol, 2022, 19 (10): 625- 637.
35	GEERLINGS S Y , KOSTOPOULOS I , DE VOS W M.D , et al. Akkermansia muciniphila in the human gastrointestinal tract: when, where, and how?[J]. Microorganisms, 2018, 6 (8): 75.
36	YANG W X , BAI X Y , LUAN X H , et al. Delicate regulation of IL-1β-mediated inflammation by cyclophilin A[J]. Cell Rep, 2022, 38 (11): 110513.
37	QING H , DESROULEAUX R , ISRANI-WINGER K , et al. Origin and function of stress-induced IL-6 in murine models[J]. Cell, 2020, 182 (2): 372- 387.
38	CHO C E , CAUDILL M A C . Trimethylamine-N-oxide: friend, foe, or simply caught in the cross-fire?[J]. Trends Endocrinol Metab, 2017, 28 (2): 121- 130.
39	SANCHEZ-LOPEZ E , ZHONG Z Y , STUBELIUS A , et al. Choline uptake and metabolism modulate macrophage IL-1β and IL-18 production[J]. Cell Metab, 2019, 29 (6): 1350- 1362.
40	LAI Y S , TANG H E , ZHANG X R , et al. Trimethylamine-N-oxide aggravates kidney injury via activation of p38/MAPK signaling and upregulation of HuR[J]. Kidney Blood Press Res, 2022, 47 (1): 61- 71.
41	PINHO J , MARQUES S A , FREITAS E , et al. Red cell distribution width as a predictor of 1-year survival in ischemic stroke patients treated with intravenous thrombolysis[J]. Thromb Res, 2018, 164, 4- 8.
42	YANG C C , ZHAO Y , REN D Y , et al. Protective effect of saponins-enriched fraction of Gynostemma pentaphyllum against high choline-induced vascular endothelial dysfunction and hepatic damage in mice[J]. Biol Pharm Bull, 2020, 43 (3): 463- 473.
43	MACHADO V S , OIKONOMOU G , LIMA S F , et al. The effect of injectable trace minerals (selenium, copper, zinc, and manganese) on peripheral blood leukocyte activity and serum superoxide dismutase activity of lactating Holstein cows[J]. Vet J, 2014, 200 (2): 299- 304.
44	ROSA C G S , COLARES J R , DA FONSECA S R B , et al. Sarcopenia, oxidative stress and inflammatory process in muscle of cirrhotic rats-Action of melatonin and physical exercise[J]. Exp Mol Pathol, 2021, 121, 104662.

项目 Item	体积/质量 Volume/mass	成分 Component
缓冲液A Buffer A	50 mL	每100 mL含K₂HPO₄ 0.3 g，4 ℃保存
缓冲液B Buffer B	50 mL	每100 mL含(NH₄)₂SO₄ 0.6 g，MgSO₄·7H₂O 0.06 g，KH₂PO₄ 0.3 g， CaCl₂·2H₂O 0.06 g，NaCl 0.6 g，4 ℃保存
基础培养液Basal medium	400 mL	2 g胰蛋白胨、2 g酵母膏、5 g NaHCO₃
无细胞瘤胃液Cell-free rumen fluid	200 mL
NH₄Cl Ammonium chloride	1 g
辅酶M Coenzyme M	10 mL	每100 mL含辅酶M 0.4 g，4 ℃保存
脂肪酸溶液Fatty acid solution	50 mL
微量元素溶液Trace element solution	10 mL
还原剂Reductant	1 g	L-半胱氨酸盐酸盐
指示剂Indicator	1 mL	0.1% 刃天青1 mL(w/v)

类别 Categories	引物序列(5′→3′) Primers sequences (5′→3′)	参考文献 References
古菌Archaea	915f-AAG AAT TGG CGG GGG AGC AC	Jeyanathan等^[21]
	1386r-GCG GTG TGT GCA AGG AGC
甲烷马赛球菌Mmc	762f-GAC GAA GCC CTG GGT C	Jeyanathan等^[21]
	1099r-GAG GGT CTC GTT CGT TAT
胆碱降解菌基因cutC	389-aa-f-TTY GCI GGI TAY CAR CCN TT	Martínez-del Campo等^[22]
Choline degrading bacteria gene cutc	492-aa-r-TGN GGR TCI ACY CAI CCC AT

项目Item	对照组 Control	处理组 Treatment	标准误 SEM	P值 P-value
总古菌/lg10(copies·g^-1) Total archaea	5.50	5.90	0.31	0.536
甲烷马赛球菌/lg10(copies·g^-1) Mmc	3.81	4.67	0.22	0.048
甲烷马赛球菌占古菌比例/% The proportion of Mmc to archaea	3.85	11.73	4.16	0.083
细菌cutC/lg10(copies·g^-1) Bacterial CutC	7.06	6.96	0.05	0.394

项目 Items	对照组 Control	处理组 Treatment	标准误 SEM	P 值 P-value
OTUs	4 299.3	5 000.1	571.3	0.191
Chao指数Chao index	4 843.9	5 636.5	493.3	0.181
Shannon指数Shannon index	8.79	9.41	0.47	0.214
Simpson指数Simpson index	0.024	0.017	0.007	0.386

门水平 Phylums	对照组 Control	处理组 Treatment	标准误 SEM	P值 P-value
Firmicutes	67.61	70.73	2.24	0.277
Bacteroidetes	15.94	19.27	2.60	0.277
Verrucomicrobia	11.63	4.27	2.01	0.064
Proteobacteria	3.67	4.45	0.73	0.949
Actinobacteria	0.63	0.68	0.15	0.848
Unclassified	0.19	0.26	0.02	0.110
Deferribacteres	0.16	0.18	0.05	0.655

甲烷马赛球菌DZ1对小鼠血清氧化三甲胺和炎症因子、肝脏抗氧化能力及盲肠微生物区系的影响

Effects of Methanomassiliicoccus DZ1 on Serum Trimethylamine-N-oxide and Inflammatory Factors, Liver Antioxidant Capacity and Cecum Microbiota in Mice

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 44

相关文章 15

编辑推荐

Metrics

本文评价

门水平 Phylums	属水平 Genus	对照组 Control	处理组 Treatment	标准误 SEM	P 值 P-value
Firmicutes	Kineothrix	10.81	8.64	2.88	0.456
	Duncaniella	7.99	9.54	2.73	0.535
	Enterocloster	6.78	4.30	1.36	0.097
	Ligilactobacillus	6.30	6.28	3.19	0.456
	Limosilactobacillus	6.05	12.15	2.96	0.128
	Muribaculum	4.50	4.49	2.18	0.456
	Lactobacillus	3.96	9.33	3.26	0.710
	Eisenbergiella	3.40	2.67	1.04	0.710
	Anaerostipes	2.32	0.69	0.99	0.073
	Schaedlerella	2.24	2.06	0.87	0.710
	Acetatifactor	2.12	1.15	0.58	0.073
	Herbivorax	1.99	1.12	0.83	0.456
	Faecalimonas	1.26	1.94	0.38	0.165
	Lacrimispora	1.20	1.19	0.24	0.902
	Herbinix	1.14	0.17	0.58	0.053
	Dubosiella	1.10	1.15	1.11	0.710
	Lawsonibacter	1.07	0.57	0.23	0.026
	Extibacter	1.05	1.71	0.46	0.128
	Ruminococcus	1.05	0.33	0.36	0.038
	Roseburia	1.04	1.04	0.34	1.000
	Hungatella	1.02	0.65	0.24	0.209
	Blautia	1.00	1.12	0.18	0.456
	Anaerocolumna	0.94	2.04	1.05	0.318
	Mediterraneibacter	0.73	1.10	0.13	1.000
Verrucomicrobia	Akkermansia	11.63	4.26	3.28	0.007
Proteobacteria	Desulfovibrio	2.99	3.49	1.64	0.073
Bacteroidota	Odoribacter	0.54	1.05	0.54	0.620
	Paramuribaculum	0.80	1.07	0.42	0.902

[1]	李碧波, 吴克, 师晓龙, 闫奕凝, 李嘉豪, 段国庆, 李熊, 任彦鹏, 董佳宁, 张春香, 任有蛇. 羊源Lactobacillus plantarum对腹泻羔羊空肠菌群及肠道黏膜屏障的调控作用[J]. 畜牧兽医学报, 2024, 55(8): 3552-3569.
[2]	宋云方, 程浩, 冯露雅, 白平, 邓远坤, 夏耀耀, 谭碧娥, 王婧. 营养调控肠道免疫细胞活化机制研究进展[J]. 畜牧兽医学报, 2024, 55(7): 2846-2858.
[3]	陈倩玲, 沙玉柱, 刘秀, 邵鹏阳, 王翻兄, 陈小伟, 杨文鑫, 谢转回, 高敏, 黄薇. 肠道微生物与线粒体互作调控动物脂肪沉积的研究进展[J]. 畜牧兽医学报, 2024, 55(6): 2293-2303.
[4]	冯铭, 伊旭东, 庞卫军. 肠道微生物通过骨骼肌纤维类型、肌内脂肪含量和骨骼肌代谢调控猪肉质研究进展[J]. 畜牧兽医学报, 2024, 55(6): 2304-2312.
[5]	徐兰梦, 黄榆智, 韩玉竹, 李常营, 章杰. 肠道微生物调控脂肪沉积及其代谢相关疾病的研究进展[J]. 畜牧兽医学报, 2024, 55(10): 4263-4277.
[6]	徐成, 田文杰, 马月辉, 王圣楠, 蒋琳, 王丹丹. 基于16S rRNA测序分析敲除基因ZBED6后巴马猪肠道微生物菌群的变化[J]. 畜牧兽医学报, 2024, 55(10): 4302-4310.
[7]	郑先瑞, 卓明雪, 纪金丽, 蒋维虎, 邓在双, 张吉成, 田雅莉, 丁月云, 张晓东, 殷宗俊. 皖南黑猪不同生长阶段血清免疫指标及肠道菌群的特征分析[J]. 畜牧兽医学报, 2023, 54(9): 3770-3783.
[8]	禹世雄, 魏凌云, 徐甜甜, 焦金真, 蒋林树, 贺志雄. 幼龄反刍动物肠道微生物定植规律及其营养调控研究进展[J]. 畜牧兽医学报, 2023, 54(7): 2701-2707.
[9]	纪鹏, 张斌, 张春勇, 邢笑锟, 杨佳, 刘韶娜, 方碟, 潘洪彬, 赵彦光, 安清聪. 日粮添加乳铁蛋白对断奶仔猪肠道微生物多样性的影响[J]. 畜牧兽医学报, 2023, 54(7): 2942-2955.
[10]	杜海东, 娜仁花. 反刍动物胃肠道上皮屏障功能及与微生物互作研究[J]. 畜牧兽医学报, 2023, 54(5): 1804-1814.
[11]	刘燕坤, 罗润波, 林焱, 朱伟云. 噬菌体鸡尾酒对断奶仔猪生长性能、血液参数和粪样菌群的影响[J]. 畜牧兽医学报, 2023, 54(4): 1555-1567.
[12]	陆梦琪, 杨文杰, 李萍, 余鹏, 董翎, 牛晓玉, 杨克礼, 邹维华, 宋卉. 基于16S rRNA测序分析PRRSV感染仔猪肺和肠道中微生物菌群的变化[J]. 畜牧兽医学报, 2023, 54(4): 1664-1678.
[13]	袁岩聪, 何航, 刘安芳, 万堃, 章杰. 不同体重四川白鹅消化生理、免疫和肠道微生物的比较分析[J]. 畜牧兽医学报, 2023, 54(3): 1124-1134.
[14]	刘攀, 李瑞琦, 谭占坤, 王逸飞, 陈晓晨, 何伟先, 杜忍让, 马健, 褚瑰燕, 蔡传江. 高纤维日粮对生长育肥猪生长性能、肉品质及肠道微生物的影响[J]. 畜牧兽医学报, 2023, 54(10): 4247-4259.
[15]	袁铜, 黄靓, 杨琳, 王文策, 朱勇文. 肠道菌群及其代谢产物调节动物线粒体功能的研究进展[J]. 畜牧兽医学报, 2023, 54(1): 48-57.