阿司匹林丁香酚酯对蛋鸡脂肪肝出血综合征的预防作用

doi:10.11843/j.issn.0366-6964.2025.06.039

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (6): 2968-2977.doi: 10.11843/j.issn.0366-6964.2025.06.039

阿司匹林丁香酚酯对蛋鸡脂肪肝出血综合征的预防作用

言凯¹^,²(), 许笑², 秦哲², 白莉霞², 李准², 杨亚军², 刘希望², 李世宏², 葛闻博²^,*(), 李剑勇¹^,²^,*(), 李存¹^,*()

1. 天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室, 天津 300392
2. 中国农业科学院兰州畜牧与兽药研究所农业农村部兽用药物创制重点实验室/甘肃省新兽药工程重点实验室, 兰州 730050

收稿日期:2024-07-29 出版日期:2025-06-23 发布日期:2025-06-25
通讯作者: 葛闻博,李剑勇,李存 E-mail:jinngoi17@gmail.com;gewenbo@caas.cn;lijy1971@163.com;hhlicun@163.com
作者简介:言凯(1997-)，男，湖南株洲人，硕士生，主要从事药理毒理学研究，E-mail: jinngoi17@gmail.com
基金资助:
甘肃省科技计划资助(25ZDNA003)；国家自然科学基金青年基金(32402950)；中央公益性科研院所基本科研业务费(1610322023008)

Preventive Effect of Aspirin Eugenol Ester on Fatty Liver Hemorrhagic Syndrome in Laying Hens

YAN Kai¹^,²(), XU Xiao², QING Zhe², BAI Lixia², LI Zhun², YANG Yajun², LIU Xiwang², LI Shihong², GE Wenbo²^,*(), LI Jianyong¹^,²^,*(), LI Cun¹^,*()

1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China
2. Key Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China

Received:2024-07-29 Online:2025-06-23 Published:2025-06-25
Contact: GE Wenbo, LI Jianyong, LI Cun E-mail:jinngoi17@gmail.com;gewenbo@caas.cn;lijy1971@163.com;hhlicun@163.com

摘要/Abstract

摘要：

旨在探讨阿司匹林丁香酚酯(AEE)对蛋鸡脂肪肝出血综合征(fatty liver hemorrhagic syndrome，FLHS)的预防作用。将78羽160日龄的海兰褐蛋鸡，随机分为6组，每组13只鸡。Ⅰ组饲喂基础日粮；Ⅱ~Ⅴ组饲喂高能低蛋白质日粮，并分别在饲料中添加0、25、50、100 mg·kg^-1阿司匹林丁香酚酯；Ⅵ组饲喂含有氯化胆碱2 g·kg^-1的饲料，试验期90 d。结果显示：1)相较于Ⅰ组，Ⅱ组产蛋率和平均蛋重显著降低(P < 0.05)，料蛋比显著升高(P < 0.05)；相较于Ⅱ组，在不同时间段Ⅲ~Ⅵ组产蛋率和平均蛋重显著升高(P < 0.05)，料蛋比显著降低(P < 0.05)。2)相较于Ⅰ组，Ⅱ组蛋鸡体重显著增加(P < 0.05)；相较于Ⅱ组，Ⅲ~Ⅵ组体重显著降低(P < 0.05)。3)相较于Ⅰ组，Ⅱ组蛋鸡肝脏明显肿大，呈土黄色，表面有点状出血，质脆易碎；HE染色结果显示，肝细胞呈紊乱排列，并出现不同大小的脂肪空泡；相较于Ⅱ组，Ⅲ~Ⅵ组肝脏结构恢复正常。4)相较于Ⅰ组，Ⅱ组蛋鸡肝指数、血清和肝脏中三酰甘油(TG)、胆固醇(TC)、低密度脂蛋白(LDL)水平显著升高，高密度脂蛋白(HDL)水平显著降低(P < 0.05)；相较于Ⅱ组，Ⅲ~Ⅵ组肝指数、血清和肝脏中TG、TC、LDL水平显著降低，HDL水平显著增加(P < 0.05)。5)相较于Ⅰ组，Ⅱ组蛋鸡肝脏中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH)、过氧化氢酶(CAT)活性显著降低，丙二醛(MDA)水平显著升高(P < 0.05)；相较于Ⅱ组，Ⅲ~Ⅵ组SOD、CAT、GSH活性显著增加，MDA水平显著减少(P < 0.05)。综上，饲喂蛋鸡高能低蛋白日粮可以诱导蛋鸡脂肪肝出血综合征病理模型；添加低、中、高剂量AEE在高能低蛋白日粮对蛋鸡脂肪肝出血综合征均起到一定的预防效果。

关键词: 阿司匹林丁香酚酯, 蛋鸡, 脂肪肝出血综合征, 抗氧化指标

Abstract:

This study aimed to investigate the preventive effects of aspirin eugenol ester (AEE) on fatty liver hemorrhagic syndrome (FLHS) in laying hens. A total of seventy-eight 160-day-old Hy-Line Brown laying hens were randomly assigned to six groups, each containing 13 hens. Results were as follows: Group Ⅰ received a basal diet, while Groups Ⅱ to Ⅴ were fed a high-energy, low-protein diet supplemented with 0, 25, 50, and 100 mg·kg^-1 of AEE, respectively. Group Ⅵ was provided a diet containing 2 g·kg^-1 choline chloride. The experiment lasted for 90 days. The results are in the following fashion: ① Compared to Group Ⅰ, Group Ⅱ showed a significant decrease in egg production rate and average egg weight (P < 0.05), and a significant increase in the feed-to-egg ratio (P < 0.05). In comparison to Group Ⅱ, Groups Ⅲ to Ⅵ exhibited a significant increase in egg production rate and average egg weight at different time points (P < 0.05), and a significant decrease in the feed-to-egg ratio (P < 0.05). ② Compared to Group Ⅰ, the body weight of hens in Group Ⅱ significantly increased (P < 0.05). However, compared to Group Ⅱ, the body weights of hens in Groups Ⅲ to Ⅵ significantly decreased (P < 0.05). ③ Compared to Group Ⅰ, the hens in Group Ⅱ showed significantly enlarged livers with a yellowish color, punctate hemorrhages on the surface, and increased friability. HE staining revealed disordered hepatocyte arrangement and the presence of fat vacuoles of various sizes. In contrast, the liver structure of hens in Groups Ⅲ to Ⅵ returned to normal compared to Group Ⅱ. ④ Liver Index and Lipid Levels: Compared to Group Ⅰ, Group Ⅱ showed significantly increased liver index and elevated levels of triglyceride (TG), total cholesterol (TC), and low-density lipoprotein (LDL) in serum and liver tissues, with significantly decreased high-density lipoprotein (HDL) levels (P < 0.05). However, compared to Group Ⅱ, Groups Ⅲ to Ⅵ exhibited significantly lower liver index, TG, TC, and LDL levels, and significantly increased HDL levels (P < 0.05). ⑤ Group Ⅱ exhibited significantly lower activities of liver superoxide dismutase (SOD), glutathione peroxidase (GSH), and catalase (CAT), along with significantly higher malondialdehyde (MDA) levels compared to Group Ⅰ (P < 0.05). In comparison, Groups Ⅲ to Ⅵ had significantly higher SOD, GSH, and CAT activities and lower MDA levels compared to Group Ⅱ (P < 0.05). The results indicate that feeding laying hens a high-energy, low-protein diet effectively induces fatty liver hemorrhagic syndrome. Supplementing this diet with varying doses of AEE demonstrates a protective effect against FLHS in laying hens.

Key words: aspirin eugenol ester, laying hens, fatty liver hemorrhagic syndrome, antioxidant markers

中图分类号:

S859.7

言凯, 许笑, 秦哲, 白莉霞, 李准, 杨亚军, 刘希望, 李世宏, 葛闻博, 李剑勇, 李存. 阿司匹林丁香酚酯对蛋鸡脂肪肝出血综合征的预防作用[J]. 畜牧兽医学报, 2025, 56(6): 2968-2977.

YAN Kai, XU Xiao, QING Zhe, BAI Lixia, LI Zhun, YANG Yajun, LIU Xiwang, LI Shihong, GE Wenbo, LI Jianyong, LI Cun. Preventive Effect of Aspirin Eugenol Ester on Fatty Liver Hemorrhagic Syndrome in Laying Hens[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2968-2977.

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项目Items	含量Content
项目Items	基础日粮 Basic diet	高能低蛋白日粮 High-energy low-protein diet
原料Ingredients
玉米Corn	64.00	70.00
豆粕Soybean meal	26.00	15.78
豆油Soybean oil		4.22
钙Calcium	8.00	8.00
预混料Premix^*	2.00	2.00
合计Total	100.00	100.00
营养水平Nutrient level
代谢能ME/(kJ·kg^-1)	11 248.64	13 212.22
粗蛋白质CP	15.50	12.30
有效磷AP	0.53	0.51
精氨酸Arg	1.03	0.74
蛋氨酸Met	0.37	0.32
缬氨酸Val	0.77	0.58
蛋氨酸+半胱氨酸Met+Cys	0.67	0.56

时间/d Time	项目Item	组别Groups
时间/d Time	项目Item	Ⅰ	Ⅱ	Ⅲ	Ⅳ	Ⅴ	Ⅵ
1~30	产蛋率/% Reproductive performance	91.92±7.25^a	85.94±9.39^b	88.75±4.42^ab	90.61±7.12^ab	92.31±7.77^a	90.49±8.70^ab
	料蛋比 Feed conversion ratio	2.20±0.55^ab	2.42±0.48^a	2.16±0.28^b	2.21±0.34^ab	2.06±0.33^b	2.28±0.55^ab
	蛋重/g Egg weight	56.47±1.42^a	54.83±1.92^c	55.11±1.92^bc	54.55±2.13^c	55.91±2.24^ab	54.60±1.72^c
31~60	产蛋率/% Reproductive performance	90.59±7.01^a	74.64±13.53^b	85.98±8.51^a	84.38±13.01^a	88.85±9.19^a	84.31±9.98^a
	料蛋比 Feed conversion ratio	2.22±0.45^b	2.61±0.90^a	2.21±0.55^b	2.36±0.47^ab	2.14±0.35^b	2.24±0.34^b
	蛋重/g Egg weight	56.74±1.68^ab	54.44±2.08^c	57.20±0.83^a	56.41±1.08^ab	56.70±0.96^ab	55.10±1.5^bc
61~90	产蛋率/% Reproductive performance	90.28±6.63^a	77.56±10.21^c	82.81±8.84^bc	85.83±11.18^ab	86.90±11.95^ab	81.28±8.44^bc
	料蛋比 Feed conversion ratio	2.24±0.31^bc	2.69±0.44^a	2.39±0.45^b	2.39±0.62^b	2.11±0.26^c	2.40±0.37^b
	蛋重/g Egg weight	59.52±1.54^a	57.78±2.18^b	58.13±2.01^b	58.83±1.67^ab	58.97±1.60^ab	58.19±2.09^b

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