不同年龄牦牛肺脏中JAK2、STAT3、P-JAK2/STAT3及PCNA蛋白的表达分析

doi:10.11843/j.issn.0366-6964.2025.06.038

摘要/Abstract

摘要：

本研究旨在探究JAK2/STAT3信号通路及其调节的细胞增殖关键蛋白PCNA在不同年龄牦牛肺脏中的表达分布及可能发挥的作用。本试验以初生、幼年、成年和老年健康牦牛肺脏为研究对象，利用实时荧光定量PCR (quantitative real-time PCR，qRT-PCR)检测JAK2、STAT3和PCNA mRNA在不同年龄牦牛肺脏中的表达情况，采用免疫组织化学(immunohistochemistry，IHC)和免疫蛋白印迹(Western blot)方法检测JAK2、STAT3、P-JAK2/STAT3及PCNA蛋白在不同年龄牦牛肺脏中的表达分布情况。结果显示：JAK2、STAT3、P-JAK2/STAT3和PCNA的表达分布基本一致，主要分布在支气管上皮与血管内皮，支气管壁平滑肌层、血管平滑肌以及肺泡细胞等部位。JAK2 mRNA的表达量在不同年龄牦牛肺脏中呈现先升高后降低趋势，成年组表达最高；STAT3 mRNA在肺脏中的表达量随牦牛年龄的增加而增加，老年组表达最高；PCNA mRNA在成年组和老年组的表达量明显高于初生组及幼年组。此外，JAK2、STAT3、P-JAK2/STAT3及PCNA蛋白在不同年龄牦牛肺脏中表达不同，P-JAK2/STAT3与JAK2/STAT3趋势基本一致，JAK2、P-JAK2蛋白在幼年组表达量最高，STAT3、P-STAT3蛋白在初生组和成年组表达量显著高于幼年组及老年组，其中初生组高于成年组；PCNA蛋白在初生组表达量明显高于幼年、成年和老年三组。综上，JAK2/STAT3信号通路及其调节的PCNA在不同年龄牦牛肺脏中的表达分布存在差异，提示JAK2/STAT3信号通路参与了牦牛肺脏发育过程。研究结果为进一步明确JAK2/STAT3信号通路及其靶向调节的PCNA在牦牛肺脏对高原低氧适应过程中发挥的作用提供研究依据。

关键词: 牦牛, 肺脏, JAK2/STAT3信号通路, 低氧性肺动脉高压

Abstract:

This study aimed to investigate the expression distribution and possible roles of the JAK2/STAT3 signaling pathway and its regulated PCNA, a key protein for cell proliferation, in the lungs of yaks of different ages. In this experiment, the lungs of newborn, juvenile, adult and aged healthy yaks were used to detect the expression of JAK2, STAT3 and PCNA mRNA in the lungs of yaks of different ages by quantitative real-time fluorescence PCR (qRT-PCR), and the expression of JAK2, STAT3, P-JAK2/STAT3 and PCNA proteins in the lungs of yaks of different ages was detected by Immunohistochemistry (IHC) and Western blot methods. The expression distribution of JAK2, STAT3, P-JAK2/STAT3 and PCNA were basically consistent, these are mainly distributed in the bronchial epithelium and vascular endothelium, the smooth muscle layer of the bronchial wall, vascular smooth muscle and alveolar cells. The expression of JAK2 mRNA in the lungs of yaks at different ages demonstrated a tendency of increasing and then decreasing, and the expression of JAK2 mRNA in the adult group was the highest. The expression of STAT3 mRNA in the lungs increased with the age of yaks, and the expression was highest in the old group. The expression of PCNA mRNA in the adult and old groups was significantly higher than that in the newborn and juvenile groups. Additionally, the expressions of JAK2, STAT3, P-JAK2/STAT3 and PCNA proteins were different in the lungs of yaks at different ages. The expression trend of P-JAK2/STAT3 was found to be essentially identical to that of JAK2/STAT3. The expression of JAK2/P-JAK2 proteins was the highest in the juvenile group, and the expression of STAT3/P-STAT3 proteins in the newborn and adult groups was significantly higher than that in the juvenile and old groups, and the newborn group was higher than that in the adult group. The expression of the PCNA protein in the newborn group was significantly higher than that in the juvenile, adult, and old groups. The findings of this study indicated that there were differences in the expression and distribution of JAK2/STAT3 signaling pathway and its regulated PCNA in yak lungs of different ages, suggesting that the JAK2/STAT3 signaling pathway plays a role in the development of yak lungs. This study provides a research foundation for further clarifying the role of the JAK2/STAT3 signaling pathway and its targeted regulation of PCNA in the adaptation of yak lungs to the plateau environment.

Key words: yak, lung, JAK2/STAT3 signaling pathway, hypoxic pulmonary hypertension

中图分类号:

S852.1

牛悦悦, 崔燕, 余四九, 何俊峰, 杨珊珊, 祁正满, 豆婉婉, 陈春燕, 邓演江. 不同年龄牦牛肺脏中JAK2、STAT3、P-JAK2/STAT3及PCNA蛋白的表达分析[J]. 畜牧兽医学报, 2025, 56(6): 2957-2967.

NIU Yueyue, CUI Yan, YU Sijiu, HE Junfeng, YANG Shanshan, QI Zhengman, DOU Wanwan, CHEN Chunyan, DENG Yanjiang. Analysis of JAK2, STAT3, P-JAK2/STAT3 and PCNA Proteins Expression in the Lungs of Yaks of Different Ages[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2957-2967.

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基因名称Gene name	引物序列(5′→3′) Primer sequences	退火温度/℃ Tm	产物大小/bp Product size	GenBank登录号 GenBank accession number
JAK2	F: TATGGAGTATGTGTCTGTGGAGAGGAG R: TTCCTGTCTTCCTGTCTTCTTCTCTGA	61.0	235	XM_024996127.2
STAT3	F: GAGAAGGACATCAGCGGCAAGAC R: GATAGACCAGTGGAGACACCAGGAT	60.0	145	NM_001012671.2
PCNA	F: CTCGTCTCATGTCTCCTTGGT R: TGTCTTCATTGCCAGCACATT	56.4	134	XM_005906528.2
β-actin	F: CCGTGACATCAAGGACAAG R: AGGAAGGAAGGATGGAAG	60.0	174	NM_173979.3

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