金黄色葡萄球菌异质性耐药研究进展

doi:10.11843/j.issn.0366-6964.2024.04.009

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (4): 1432-1445.doi: 10.11843/j.issn.0366-6964.2024.04.009

金黄色葡萄球菌异质性耐药研究进展

和晓兰¹, 赵艳坤^1,2, 孟璐¹, 刘慧敏¹, 高姣姣², 郑楠^1*

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 新疆农业科学院农业质量标准与检测技术研究所农业农村部农产品质量安全风险评估实验室(乌鲁木齐)新疆农产品质量安全实验室, 乌鲁木齐 830091

收稿日期:2023-07-10 出版日期:2024-04-23 发布日期:2024-04-26
通讯作者: 郑楠,主要从事奶产品风险评估与营养功能评价研究,E-mail:zhengnan@caas.cn
作者简介:和晓兰(2001-)，女，河北内丘人，硕士生，主要从事食品科学研究，E-mail:2460213483@qq.com
基金资助:
国家重点研发计划(2022YFD1301003);国家现代农业产业技术体系(CARS36);农产品质量安全风险评估(GJFP20220304);中国农业科学院科技创新工程(ASTIP-IAS12);国家农产品质量安全风险评估项目(14225001);国家自然科学基金(32060797);天山创新团队(2022D14016)

Research Progress in Heteroresistance of Staphylococcus aureus

HE Xiaolan¹, ZHAO Yankun^1,2, MENG Lu¹, LIU Huimin¹, GAO Jiaojiao², ZHENG Nan^1*

1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Ministry of Agriculture and Rural Affairs-Laboratory of Quality and Safety Risk Assessment for Agro-Products(Urumgi), Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2023-07-10 Online:2024-04-23 Published:2024-04-26

摘要/Abstract

摘要： 金黄色葡萄球菌(Staphylococcus aureus，S. aureus)是人类和动物感染的常见病原菌。近年来，金黄色葡萄球菌已经对多种抗菌药物出现耐药性，关于金黄色葡萄球菌异质性耐药的研究也被频繁报道。异质性耐药现象是指存在异质性细菌种群，其中一个亚群或几个亚群与主要种群相比表现出更高水平的抗菌药物耐药性。该定义反映出异质性耐药现象具有很多特点，包括克隆性、稳定性、不同亚群的耐药水平以及异质性耐药亚群的频率应在10^-7及以上。异质性耐药可看成是敏感表型向耐药表型转化的中间阶段，往往导致临床治疗的失败。本文对异质性耐药的定义、检测方法等进行了介绍，并综述了金黄色葡萄球菌对糖肽类、磺胺类、β-内酰胺类和其他抗菌药物异质性耐药的流行情况及异质性耐药机制的研究，旨在加强对金黄色葡萄球菌异质性耐药的认识，以期为学者了解金黄色葡萄球菌异质性耐药流行情况及机制提供参考。

关键词: 金黄色葡萄球菌, 异质性耐药, 抗菌药物, 流行情况, 机制

Abstract: Staphylococcus aureus (S. aureus) is a common cause of infection in humans and animals. In recent years, S. aureus has become resistant to a variety of antimicrobial drugs, and studies on heteroresistance in S. aureus have been frequently reported. The broadest definition of heteroresistance is the presence of a heterogeneous population of bacteria with one subpopulation or several subpopulations that exhibit increased levels of antibiotic resistance compared with the main population. However, this definition includes phenomena with very different characteristics; for example, clonality of resistant subpopulations, the stability of the resistant subpopulations, the resistance level of the resistant subpopulations, and at a frequency greater than 1×10^-7. Heteroresistance is the intermediate stage of sensitive bacteria evolving into drug-resistant bacteria, which often leads to the failure of clinical therapy. This paper introduces the definition of heteroresistance, detection methods, etc., and reviews the prevalence of heteroresistance of S. aureus to glycopeptides, sulfonamides, β-lactams, and other antibacterial agents and the study of the mechanism of heteroresistance. It is aimed to strengthen the understanding on the heteroresistance of S. aureus, and to provide a reference for scholars to understand the prevalence and mechanism of heteroresistance of S. aureus.

Key words: Staphylococcus aureus, heteroresistance, antibiotics, prevalence, mechanism

中图分类号:

S852.611

和晓兰, 赵艳坤, 孟璐, 刘慧敏, 高姣姣, 郑楠. 金黄色葡萄球菌异质性耐药研究进展[J]. 畜牧兽医学报, 2024, 55(4): 1432-1445.

HE Xiaolan, ZHAO Yankun, MENG Lu, LIU Huimin, GAO Jiaojiao, ZHENG Nan. Research Progress in Heteroresistance of Staphylococcus aureus[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1432-1445.

参考文献

[1] LUKASSEK J, IGNATOV A, FAERBER J, et al. Puerperal mastitis in the past decade:results of a single institution analysis[J]. Arch Gynecol Obstet, 2019, 300(6):1637-1644.
[2] BRANCH-ELLIMAN W, GOLEN T H, GOLD H S, et al. Risk factors for Staphylococcus aureus postpartum breast abscess[J]. Clin Infect Dis, 2012, 54(1):71-77.
[3] MOORE J, GOODAY C, SOLIMAN R, et al. Reduction in the prevalence of methicillin-resistant Staphylococcus aureus in tissue and wound swab samples taken from outpatients attending a specialist diabetic foot clinic 2005-2021[J]. Diabet Med, 2023, 40(10):e15081.
[4] RUSSELL S P, NEARY C, ABD ELWAHAB S, et al. Breast infections-microbiology and treatment in an era of antibiotic resistance[J]. Surgeon, 2020, 18(1):1-7.
[5] ENANY S, ALEXANDER L C. The rise of virulence and antibiotic resistance in Staphylococcus aureus[M]. Croatia, InTech; 2016:25-41.
[6] KIRMUSAOǦLU S, GAREAYAGHI N, KOCAZEYBEK B S. Antimicrobials, antibiotic resistance, antibiofilm strategies and activity methods[M]. London, Intech, 2019:3-12.
[7] SATOLA S W, FARLEY M M, ANDERSON K F, et al. Comparison of detection methods for heteroresistant vancomycin-intermediate Staphylococcus aureus, with the population analysis profile method as the reference method[J]. J Clin Microbiol, 2011, 49(1):177-183.
[8] Centers for Disease Control and Prevention (CDC). Interim guidelines for prevention and control of staphylococcal infection associated with reduced susceptibility to vancomycin[R]. MMWR Morb Mortal Wkly Rep, 1997, 46(27):626-628, 635.
[9] SAKOULAS G, MOISE-BRODER P A, SCHENTAG J, et al. Relationship of MIC and bactericidal activity to efficacy of vancomycin for treatment of methicillin-resistant Staphylococcus aureus bacteremia[J]. J Clin Microbiol, 2004, 42(6):2398-2402.
[10] BAND V I, WEISS D S. Heteroresistance to beta-lactam antibiotics may often be a stage in the progression to antibiotic resistance[J]. PLoS Biol, 2021, 19(7):e3001346.
[11] ANDERSSON D I, NICOLOFF H, HJORT K. Mechanisms and clinical relevance of bacterial heteroresistance[J]. Nat Rev Microbiol, 2019, 17(8):479-496.
[12] DEWACHTER L, FAUVART M, MICHIELS J. Bacterial heterogeneity and antibiotic survival:understanding and combatting persistence and heteroresistance[J]. Mol Cell, 2019, 76(2):255-267.
[13] SUN L, TALARICO S, YAO L N, et al. Droplet digital PCR-based detection of clarithromycin resistance in Helicobacter pylori isolates reveals frequent heteroresistance[J]. J Clin Microbiol, 2018, 56(9):e00019.
[14] DAI Y C, LI C Y, YI J, et al. Plasmonic colloidosome-coupled MALDI-TOF MS for Bacterial heteroresistance study at single-cell level[J]. Anal Chem, 2020, 92(12):8051-8057.
[15] RIVERA A, VIÑADO B, BENITO N, et al. Recommendations of the Spanish antibiogram committee (COESANT) for in vitro susceptibility testing of antimicrobial agents by disk diffusion[J]. Enferm Infecc Microbiol Clin (Engl Ed), 2023, 41(9):571-576.
[16] MATUSCHEK E, COPSEY-MAWER S, PETERSSON S, et al. The European committee on antimicrobial susceptibility testing disc diffusion susceptibility testing method for frequently isolated anaerobic bacteria[J]. Clin Microbiol Infect, 2023, 29(6):795.e1-795.e7.
[17] ZUILL D E, ALMAGUER A L, DONATELLI J, et al. Development and preliminary validation of a modified EUCAST yeast broth microdilution MIC method with Tween 20-supplemented medium for rezafungin[J]. J Antimicrob Chemother, 2023, 78(4):1102-1110.
[18] ROISIN S, NONHOFF C, DENIS O, et al. Evaluation of new Vitek 2 card and disk diffusion method for determining susceptibility of Staphylococcus aureus to oxacillin[J]. J Clin Microbiol, 2008, 46(8):2525-2528.
[19] SPENCER D, LI Y T, ZHU Y L, et al. Electrical broth micro-dilution for rapid antibiotic resistance testing[J]. ACS Sens, 2023, 8(3):1101-1108.
[20] KIM J W, LEE K J. Development of a single-nucleotide polymorphism genotyping assay for the rapid detection of vancomycin-intermediate resistance in Staphylococcus aureus epidemic lineage ST5[J]. Ann Lab Med, 2023, 43(4):355-363.
[21] DING Y F, HUANG C X, CHEN M X, et al. Rapid and simultaneous detection of viable S. aureus and its penicillin susceptibility by phage amplification techniques in different food matrices[J]. LWT, 2023, 176:114526.
[22] OPERARIO D J, KOEPPEL A F, TURNER S D, et al. Prevalence and extent of heteroresistance by next generation sequencing of multidrug-resistant tuberculosis[J]. PLoS One, 2017, 12(5):e0176522.
[23] PRICE C S, KON S E, METZGER S. Rapid antibiotic susceptibility phenotypic characterization of Staphylococcus aureus using automated microscopy of small numbers of cells[J]. J Microbiol Methods, 2014, 98:50-58.
[24] GALLOIS E, FIHMAN V, DANJEAN M, et al. QMAC-dRAST for the direct testing of antibiotic susceptibility for Enterobacterales in positive blood-culture broth:a comparison of the performances with the MicroScan system and direct disc diffusion testing methods[J]. J Antimicrob Chemother, 2023, 78(3):684-691.
[25] SHIMAMOTO Y, ARAIE H, ITOH K, et al. MALDI-TOFMS-oriented early definitive therapy improves the optimal use of antibiotics for Enterococcus spp. bloodstream infection[J]. J Infect Chemother, 2021, 27(2):393-396.
[26] LIU Z Y, XUE Y, YANG C, et al. Rapid identification and drug resistance screening of respiratory pathogens based on single-cell Raman spectroscopy[J]. Front Microbiol, 2023, 14:1065173.
[27] HU Y F, FU M X, WANG F X, et al. Molecular typing and homology analysis of heterogeneous vancomycin-intermediate Staphylococcus aureus[J]. Chinese Journal of Antibiotics, 2023, 48(2):222-227. (in Chinese)
胡远芳, 付明霞, 王凤霞, 等. 异质性万古霉素中介金黄色葡萄球菌的分子分型和同源性分析[J]. 中国抗生素杂志, 2023, 48(2):222-227.
[28] LIU C L, MING L. The epidemiology of heterogeneous Vancomycin-intermediated Staphylococcus aureus and study on impact of mgrA gene in Staphylococcus aureus on vancomycin resistance[J]. Chinese Journal of Antibiotics, 2020, 45(2):175-180. (in Chinese)
刘彩林, 明亮. 异质性万古霉素中介金黄色葡萄球菌的流行性及mgrA基因对万古霉素耐药性影响的研究[J]. 中国抗生素杂志, 2020, 45(2):175-180.
[29] NAM E Y, YANG S J, KIM E S, et al. Emergence of Daptomycin-nonsusceptible Methicillin-resistant Staphylococcus aureus clinical isolates among Daptomycin-naive patients in Korea[J]. Microb Drug Resist, 2018, 24(5):534-541.
[30] SREEJISHA M, MULKI S S, SHENOY S, et al. Heterogeneous vancomycin intermediate Staphylococcus aureus infections in diabetic and non-diabetic patients:a hospital-based comparative study[J]. Infect Drug Resist, 2023, 16:9-17.
[31] AMBERPET R, SISTLA S, SUGUMAR M, et al. Detection of heterogeneous vancomycin-intermediate Staphylococcus aureus:a preliminary report from south India[J]. Indian J Med Res, 2019, 150(2):194-198.
[32] RAMLI S R, NEOH H M, AZIZ M N, et al. Screening and detection of heterogenous vancomycin intermediate Staphylococcus aureus in hospital Kuala Lumpur Malaysia, using the glycopeptide resistance detection Etest and population analysis profiling[J]. Infect Dis Rep, 2012, 4(1):e20.
[33] LIANG J, HU Y F, FU M X, et al. Resistance and molecular characteristics of methicillin-resistant Staphylococcus aureus and heterogeneous vancomycin-intermediate Staphylococcus aureus[J]. Infect Drug Resist, 2023, 16:379-388.
[34] CAMPANILE F, BORBONE S, PEREZ M, et al. Heteroresistance to glycopeptides in Italian meticillin-resistant Staphylococcus aureus (MRSA) isolates[J]. Int J Antimicrob Agents, 2010, 36(5):415-419.
[35] YAMAKAWA J, KUROSAWA H, KANEKO J, et al. Heterogeneously vancomycin-intermediate Staphylococcus aureus (hVISA) emerged before the clinical introduction of vancomycin in Japan:a retrospective study[J]. J Infect Chemother, 2012, 18(3):406-409.
[36] TROŚCIAŃCZYK A, NOWAKIEWICZ A, KASELA M, et al. Multi-host pathogen Staphylococcus aureus-epidemiology, drug resistance and occurrence in humans and animals in Poland[J]. Antibiotics (Basel), 2023, 12(7):1137.
[37] LIU R S. Effects of sub-inhibitory concentration of oxacillin on biofilm formation ability of OS-MRSA and its regulatory mechanism[D]. Hohhot:Inner Mongolia Medical University, 2022. (in Chinese)
刘柔杉. 亚抑菌浓度苯唑西林对OS-MRSA生物膜形成能力的影响及调控机制[D]. 呼和浩特:内蒙古医科大学, 2022.
[38] SARAVOLATZ S N, MARTIN H, PAWLAK J, et al. Ceftaroline-heteroresistant Staphylococcus aureus[J]. Antimicrob Agents Chemother, 2014, 58(6):3133-3136.
[39] PARDO L, GIUDICE G, MOTA M I, et al. Phenotypic and genotypic characterization of oxacillin-susceptible and mecA positive Staphylococcus aureus strains isolated in Uruguay[J]. Rev Argent Microbiol, 2022, 54(4):293-298.
[40] YU N, ZHANG W, CHEN J K, et al. Investigation of an appropriate screening method for heterogeneity of methicillin-resistant Staphylococcus aureus[J]. Medical & Pharmaceutical Journal of Chinese People's Liberation Army, 2013, 25(5):74-76. (in Chinese)
于农, 张伟, 陈建魁, 等. 耐甲氧西林金黄色葡萄球菌异质性耐药株检测方法探讨[J]. 解放军医药杂志, 2013, 25(5):74-76.
[41] GUO Z X, LI L Y. Analysis the detection methods of resistant strains of resistant Staphylococcus aureus[J]. Journal of Clinical Medical Literature, 2016, 3(14):2685-2686. (in Chinese)
郭振秀, 李凌云. 耐甲氧西林金黄色葡萄球菌(MRSA)的异质性耐药株的检测方法分析[J]. 临床医药文献电子杂志, 2016, 3(14):2685-2686.
[42] XU Z, CHEN X Y, TAN W, et al. Prevalence and antimicrobial resistance of salmonella and Staphylococcus aureus in fattening pigs in Hubei Province, China[J]. Microb Drug Resist, 2021, 27(11):1594-1602.
[43] NWOBI O C, ANYANWU M U, JAJA I F, et al. Staphylococcus aureus in horses in Nigeria:occurrence, antimicrobial, methicillin and heavy metal resistance and virulence potentials[J]. Antibiotics (Basel), 2023, 12(2):242.
[44] HUANG J W, ZHANG W G, SUN B Q, et al. Genetic diversity, antibiotic resistance, and virulence characteristics of Staphylococcus aureus from raw milk over 10 years in Shanghai[J]. Int J Food Microbiol, 2023, 401:110273.
[45] AMARE A, WORKU T, ASHAGIRIE B, et al. Bacteriological profile, antimicrobial susceptibility patterns of the isolates among street vended foods and hygienic practice of vendors in Gondar town, Northwest Ethiopia:a cross sectional study[J]. BMC Microbiol, 2019, 19(1):120.
[46] SATO T, ITO R, KAWAMURA M, et al. The risk of emerging resistance to trimethoprim/sulfamethoxazole in Staphylococcus aureus[J]. Infect Drug Resist, 2022, 15:4779-4784.
[47] NURJADI D, CHANTHALANGSY Q, ZIZMANN E, et al. Phenotypic detection of hemin-inducible trimethoprim-sulfamethoxazole heteroresistance in Staphylococcus aureus[J]. Microbiol Spectr, 2021, 9(2):e01510-21.
[48] NAKIPOǦLU Y, IǦNAK S, GÜRLER N, et al. Klinik Staphylococcus aureus suşlarında antiseptik direnç genlerinin (qacA/B ve smr) ve antibiyotik maddelere direnç prevalansının araştırılması[J]. Mikrobiyol Bul, 2012, 46(2):180-189. (in Turkish).
[49] WANG S F, YU Z N, WANG J, et al. Prevalence, drug resistance, and virulence genes of potential pathogenic bacteria in pasteurized milk of Chinese fresh milk bar[J]. J Food Prot, 2021, 84(11):1863-1867.
[50] MUTU E, CHEN G L, LIU R S, et al. High prevalence of heterogeneous mupirocin-resistant Staphylococcus aureus and its molecular characterization[J]. Am J Transl Res, 2022, 14(11):8243-8251.
[51] BAI B, LIN Z W, PU Z Y, et al. In vitro activity and heteroresistance of omadacycline against clinical Staphylococcus aureus isolates from China reveal the impact of omadacycline susceptibility by branched-chain amino acid transport system Ⅱ carrier protein, Na/Pi cotransporter family protein, and fibronectin-binding protein[J]. Front Microbiol, 2019, 10:2546.
[52] CHEN D K, LAI H Y, YANG D M, et al. Mechanism of hetero-erythromycin resistant Staphylococcus aureus and a comparison of detection methods[J]. National Medical Journal of China, 2013, 93(48):3867-3871. (in Chinese)
陈东科, 赖惠英, 杨冬梅, 等. 红霉素异质性耐药金黄色葡萄球菌耐药机制及检测方法学的比较[J]. 中华医学杂志, 2013, 93(48):3867-3871.
[53] ZHANG F, BAI B, XU G J, et al. Eravacycline activity against clinical S. aureus isolates from China:in vitro activity, MLST profiles and heteroresistance[J]. BMC Microbiol, 2018, 18(1):211.
[54] HU Q W, PENG H G, RAO X C. Molecular events for promotion of vancomycin resistance in vancomycin intermediate Staphylococcus aureus[J]. Front Microbiol, 2016, 7:1601.
[55] HU J X, CHEN L, LI G H, et al. Prevalence and genetic characteristics of fosB-positive Staphylococcus aureus in duck farms in Guangdong, China in 2020[J]. J Antimicrob Chemother, 2023, 78(3):802-809.
[56] KATAYAMA Y, MURAKAMI-KURODA H, CUI L Z, et al. Selection of heterogeneous vancomycin-intermediate Staphylococcus aureus by imipenem[J]. Antimicrob Agents Chemother, 2009, 53(8):3190-3196.
[57] HOWDEN B P, MCEVOY C R E, ALLEN D L, et al. Evolution of multidrug resistance during Staphylococcus aureus infection involves mutation of the essential two component regulator WalKR[J]. PLoS Pathog, 2011, 7(11):e1002359.
[58] SHU X Q, SHI Y Y, HUANG Y, et al. Transcription tuned by S-nitrosylation underlies a mechanism for Staphylococcus aureus to circumvent vancomycin killing[J]. Nat Commun, 2023, 14(1):2318.
[59] TUON F F, SUSS P H, TELLES J P, et al. Antimicrobial treatment of Staphylococcus aureus biofilms[J]. Antibiotics (Basel), 2023, 12(1):87.
[60] AKCAM F Z, TINAZ G B, KAYA O, et al. Evaluation of methicillin resistance by cefoxitin disk diffusion and PBP2a latex agglutination test in mecA-positive Staphylococcus aureus, and comparison of mecA with femA, femB, femX positivities[J]. Microbiol Res, 2009, 164(4):400-403.
[61] WU S, DE LENCASTRE H, TOMASZ A. Sigma-B, a putative operon encoding alternate sigma factor of Staphylococcus aureus RNA polymerase:molecular cloning and DNA sequencing[J]. J Bacteriol, 1996, 178(20):6036-6042.
[62] FISHER J F, MOBASHERY S. β-Lactams against the fortress of the gram-positive Staphylococcus aureus bacterium[J]. Chem Rev, 2021, 121(6):3412-3463.
[63] SASAKI H, ISHIKAWA H, ITOH T, et al. Penicillin-binding proteins and associated protein mutations confer oxacillin/cefoxitin tolerance in borderline oxacillin-resistant Staphylococcus aureus[J]. Microb Drug Resist, 2021, 27(5):590-595.
[64] PROULX M K, PALACE S G, GANDRA S, et al. Reversion from methicillin susceptibility to methicillin resistance in Staphylococcus aureus during treatment of bacteremia[J]. J Infect Dis, 2016, 213(6):1041-1048.
[65] LIANG B, XIONG Z, LIANG Z, et al. Genomic basis of occurrence of cryptic resistance among oxacillin-and cefoxitin-susceptible mecA-positive Staphylococcus aureus[J]. Microbiol Spectr, 2022, 10(3):e0029122.
[66] CLARKE R S, BRUDERER M S, HA K P, et al. RexAB is essential for the mutagenic repair of Staphylococcus aureus DNA damage caused by co-trimoxazole[J]. Antimicrob Agents Chemother, 2019, 63(12):e00944-19.
[67] KRIEGESKORTE A, LORÈ N I, BRAGONZI A, et al. Thymidine-dependent Staphylococcus aureus small-colony variants are induced by trimethoprim-sulfamethoxazole (SXT) and have increased fitness during SXT challenge[J]. Antimicrob Agents Chemother, 2015, 59(12):7265-7272.
[68] YU P J, PAN Y, SUN L Y, et al. Antibiotic resistance mechanism of heterogeneous linezolid-resistant Staphylococcus aureus[J]. Chinese Journal of Antibiotics, 2013, 38(3):230-234, 238. (in Chinese)
虞培娟, 潘扬, 孙兰云, 等. 异质性利奈唑胺耐药金黄色葡萄球菌耐药机制研究[J]. 中国抗生素杂志, 2013, 38(3):230-234, 238.
[69] WANG Z W, LIN Z W, BAI B, et al. Eravacycline susceptibility was impacted by genetic mutation of 30S ribosome subunits, and branched-chain amino acid transport system Ⅱ carrier protein, Na/Pi cotransporter family protein in Staphylococcus aureus[J]. BMC Microbiol, 2020, 20(1):189.
[70] STEPHEN J, SALAM F, LEKSHMI M, et al. The major facilitator superfamily and antimicrobial resistance efflux pumps of the ESKAPEE pathogen Staphylococcus aureus[J]. Antibiotics (Basel), 2023, 12(2):343.

金黄色葡萄球菌异质性耐药研究进展

Research Progress in Heteroresistance of Staphylococcus aureus

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	黄杰, 阮子豪, 蔡瑞. 抗菌肽在猪精液常温保存中的应用研究进展[J]. 畜牧兽医学报, 2024, 55(4): 1401-1411.
[2]	罗通旺, 吴亚, 王书杰, 宋厚辉, 邵春艳. 镉致肝损伤机制及硒拮抗镉肝毒性的研究进展[J]. 畜牧兽医学报, 2024, 55(4): 1456-1466.
[3]	王潇, 张昊, 栾庆江, 李慧, 杨鼎, 王婷月, 田菁, 赵濛, 陈陆, 田如刚. 冷热应激对肉牛生理指标及基因表达影响的研究进展[J]. 畜牧兽医学报, 2024, 55(3): 894-904.
[4]	高远集, 刘畅, 陈淼, 陈松彪, 张俊峰, 李静, 贾艳艳, 廖成水, 郭荣显, 丁轲, 余祖华, 尚珂. 细菌外膜囊泡结构、分泌特性及致病机制[J]. 畜牧兽医学报, 2024, 55(3): 971-983.
[5]	符雪珍, 李鑫灿, 钱虹宇, 吕红, 吴婵玉, 王晓涵, 王小华, 王芝英, 周作勇. 香柠檬精油抗伪结核棒状杆菌作用及机制研究[J]. 畜牧兽医学报, 2024, 55(3): 1217-1227.
[6]	王浩, 肖金龙, 沈珏, 赵金刚, 王帅, 刘根, 赵汝, 肖鹏, 高洪. 细胞死亡的新方式——铁死亡与铜死亡[J]. 畜牧兽医学报, 2024, 55(2): 461-470.
[7]	周梦婷, 宋银娟, 许健, 李斌, 冉多良, 储岳峰. 基于碳水化合物的佐剂作用机制研究进展[J]. 畜牧兽医学报, 2024, 55(2): 491-501.
[8]	吴自豪, 蔡依龙, 陀海欣, 陈伟. 1株马乳源PVL⁺ST22型金黄色葡萄球菌致病性分析[J]. 畜牧兽医学报, 2024, 55(2): 718-726.
[9]	刘元红, 胡玉欢, 张莉, 杨萍瑞, 胡卫东, 马琪, 毕师诚. 白术-肉苁蓉治疗便秘的网络药理学分析及试验验证[J]. 畜牧兽医学报, 2024, 55(2): 834-845.
[10]	王瑞玲, 王雪妍, 王菲菲, 孔维怡, 毛永霞, 刘欣, 丁辉, 许立华, 郭延生. 奶牛产后急性子宫内膜炎血液氧化脂质组变化特征[J]. 畜牧兽医学报, 2024, 55(1): 373-387.
[11]	何晨鹏, 李柏珍, 刘杰, 贺建华, 伍树松. 母猪繁殖障碍综合征的主要发病原因及机制研究进展[J]. 畜牧兽医学报, 2023, 54(8): 3139-3151.
[12]	王思盈, 邹宏, 宋振辉. Na⁺/H⁺交换体家族第三个亚型在感染性腹泻中的作用及活性调控机制[J]. 畜牧兽医学报, 2023, 54(8): 3230-3241.
[13]	张旭梅, 魏玉荣, 许丞惠, 杨彤, 史慧君, 付强, 杨莉. 基于网络药理学和试验验证分析小檗碱治疗鸡沙门菌感染的作用机制[J]. 畜牧兽医学报, 2023, 54(8): 3557-3570.
[14]	周炜炜, 王雪峰, 张梦洁, 杨娟, 孙悦龙, 张祖烽, 张宇欣, 豆佳红, 王梓颖, 戴小枫, 李秀梅. 基于生物网络功能模块整合组方规律分析四黄止痢颗粒治疗仔猪腹泻的作用机制[J]. 畜牧兽医学报, 2023, 54(7): 3031-3043.
[15]	安宗麒, 占思远, 李利, 张红平. circRNA作为ceRNA调控畜禽重要经济性状的研究进展[J]. 畜牧兽医学报, 2023, 54(6): 2215-2222.