冷热应激对肉牛生理指标及基因表达影响的研究进展

doi:10.11843/j.issn.0366-6964.2024.03.004

摘要/Abstract

摘要： 冷热应激可导致肉牛的日增重、饲料转化率等重要指标降低，进而影响到整个产业的生产效率，基于此问题，本文就冷热应激下不同地区、不同品种的肉牛血液内分泌、免疫、抗氧化等生理生化指标变化情况进行了概述，对冷热应激的调控基因和分子机制做了挖掘，并在讨论部分展望了包括改变饲喂方式和基于GWAS的分子育种等增加肉牛冷热应激适应性的可行性方法。本文不但为更好地理解肉牛产业中冷热应激的机制提供了理论依据，还为我国提高冷热应激下肉牛的生产效率提供了育种参考，具有一定的理论和现实意义。

关键词: 冷热应激, 肉牛, 生理生化指标, 调控基因, 分子机制

Abstract: Both cold and heat stress can negatively affect the daily gain and feed conversion rate of cattle, which could reduce the production efficiency of the beef cattle industry. This article provides a comprehensive overview of the mechanisms underlying the physiological and biochemical responses of cattle from different regions under cold and heat stress. Additionally, the molecular mechanisms and regulatory genes associated with cold and heat stress were investigated, and practical approaches to mitigate these stressors, such as altering feeding habits and molecular breeding grounded in genome-wide association studies (GWAS), were deliberated and suggested within the beef cattle sector. The review provided the theoretical basis for a better understanding of the mechanisms of cold and heat stress in the beef cattle industry, which could be used in breeding programs to improve production efficiency under cold and heat stress in China.

Key words: cold and heat stress, beef cattle, physiological and biochemical indicators, regulatory genes, molecular mechanisms

中图分类号:

S823.92

王潇, 张昊, 栾庆江, 李慧, 杨鼎, 王婷月, 田菁, 赵濛, 陈陆, 田如刚. 冷热应激对肉牛生理指标及基因表达影响的研究进展[J]. 畜牧兽医学报, 2024, 55(3): 894-904.

WANG Xiao, ZHANG Hao, LUAN Qingjiang, LI Hui, YANG Ding, WANG Tingyue, TIAN Jing, ZHAO Meng, CHEN Lu, TIAN Rugang. A Comprehensive Review of the Impact of Cold and Heat Stress on the Physiological Parameters and Gene Expression in Beef Cattle[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 894-904.

参考文献

[1] 刘曦.肉牛发生应激的防治措施[J].中国动物保健, 2021, 23(3):82-83.
LIU X.Prevention and treatment measures for stress in beef cattle[J].China Animal Health, 2021, 23(3):82-83.(in Chinese)
[2] 程玉萍, 黄子芮.不同气候条件对肉牛生长性能、血液生化及免疫指标的影响[J].中国饲料, 2021(14):17-20.
CHENG Y P, HUANG Z R.Effects of different climatic conditions on growth performance, blood biochemistry and immune indices of beef cattle[J].China Feed, 2021(14):17-20.(in Chinese)
[3] THORNTON P, NELSON G, MAYBERRY D, et al.Impacts of heat stress on global cattle production during the 21st century:a modelling study[J].Lancet Planet Health, 2022, 6(3):e192-e201.
[4] TOGHIANI S, HAY E H, ROBERTS A, et al.Impact of cold stress on birth and weaning weight in a composite beef cattle breed[J].Livest Sci, 2020, 236:104053.
[5] 毕伟波.冷应激对奶牛的影响及控制措施[J].现代畜牧科技, 2018(2):31.
BI W B.The effect and control measures of cold stress on dairy cows[J].Modern Animal Husbandry Science & Technology, 2018, 38(2):31.(in Chinese)
[6] 罗宗刚, 王玲, 蔡明成, 等.热应激对不同杂交组合肉牛生理指标和血液生化指标的影响[J].中国畜牧杂志, 2015, 51(11):82-85.
LUO Z G, WANG L, CAI M C, et al.Effects of heat stress on physiological index and blood biochemical index of different hybrid beef cattle[J].Chinese Journal of Animal Science, 2015, 51(11):82-85.(in Chinese)
[7] TOGHIANI S, HAY E, FRAGOMENI B, et al.Genotype by environment interaction in response to cold stress in a composite beef cattle breed[J].Animal, 2020, 14(8):1576-1587.
[8] 郑海英, 吴佳琦, 包雨鑫, 等.热应激对科尔沁牛与科尔沁肉牛抗氧化功能的影响[J].中国牛业科学, 2021, 47(6):16-19.
ZHENG H Y, WU J Q, BAO Y X, et al.Effect of heat stress on anti-oxidative capability in Kerqin cattle and Kerqin beef cattle[J]. China Cattle Science, 2021, 47(6):16-19.(in Chinese)
[9] 徐明, 吴淑云, 黄常宝, 等.呼和浩特地区牛舍内温湿度变化规律和奶牛冷热应激判定[J].家畜生态学报, 2015, 36(2):54-60.
XU M, WU S Y, HUANG C B, et al.Cold or heat stress determination of barn cows on temperature and humidity index in Hohhot[J].Acta Ecologae Animalis Domastici, 2015, 36(2):54-60.(in Chinese)
[10] 格日乐其木格.冷应激对放牧肉牛免疫功能和抗氧化功能的影响[J].当代畜禽养殖业, 2021(4):7-9.
GERILE Q M G.The effect of cold stress on the immune and antioxidant functions of grazing beef cattle[J].Modern Animal Husbandry, 2021(4):7-9.(in Chinese)
[11] 查森, 敖日格乐.冷应激对放牧肉牛抗氧化功能和血液指标的影响[J].当代畜禽养殖业, 2021(6):12-14.
ZHA S, AORIGELE.The effect of cold stress on antioxidant function and blood indicators in grazed beef cattle[J].Modern Animal Husbandry, 2021(6):12-14.(in Chinese)
[12] BAGATH M, KRISHNAN G, DEVARAJ C, et al.The impact of heat stress on the immune system in dairy cattle:A review[J].Res Veterinary Sci, 2019, 126:94-102.
[13] SEJIAN V, BHATTA R, GAUGHAN J B, et al.Review:Adaptation of animals to heat stress[J].Animal, 2018, 12(S2):S431-S444.
[14] MADHUSOODAN A P, SEJIAN V, RASHAMOL V P, et al.Resilient capacity of cattle to environmental challenge-An updated review[J].J Anim Behav Biometeorol, 2019, 7(3):104-118.
[15] 白丹丹.不同季节对三河牛血液生化指标及冷热应激相关基因表达的影响[D].呼和浩特:内蒙古农业大学, 2017.
BAI D D.Effect of Different seasons on blood biochemical indexes and related genes expression of cold and heat stress in Sanhe cattle[D].Hohhot:Inner Mongolia Agricultural University, 2017.(in Chinese)
[16] 陈浩, 敖日格乐, 王纯洁, 等.慢性冷应激对放牧蒙古母牛血清酶活力、蛋白代谢及血清激素分泌的影响[J].中国农业大学学报, 2019, 24(10):47-54.
CHEN H, AORIGELE, WANG C J, et al.Effects of chronic cold stress on the serum enzyme activity, protein metabolism and serum hormone secretion of grazing Mongolian cows[J].Journal of China Agricultural University, 2019, 24(10):47-54.(in Chinese)
[17] GALÁN E, LlONCH P, VILLAGRÁ A, et al.A systematic review of non-productivity-related animal-based indicators of heat stress resilience in dairy cattle[J].PLoS One, 2018, 13(11):e0206520.
[18] NEJAD J G, KIM B W, LEE B H, et al.Coat and hair color:hair cortisol and serotonin levels in lactating Holstein cows under heat stress conditions[J].Anim Sci J, 2017, 88(1):190-194.
[19] MANCUSO C, NAVARRA P, PREZIOSI P.Roles of nitric oxide, carbon monoxide, and hydrogen sulfide in the regulation of the hypothalamic-pituitary-adrenal axis[J].J Neurochem, 2010, 113(3):563-575.
[20] 陈浩, 敖日格乐, 王纯洁, 等.慢性冷热应激对放牧西门塔尔牛抗氧化功能及HPA轴、SAM轴激素分泌的影响[J].中国兽医学报, 2021, 41(1):117-123.
CHEN H, AORIGELE, WANG C J, et al.Effects of chronic cold and heat stress on the antioxidant function and hormone secretion of HPA axis and SAM axis of grazing Simmental cattle[J].Chinese Journal of Veterinary Science, 2021, 41(1):117-123.(in Chinese)
[21] LUCY M C, GARVERICK H A, SPIERS D E.Stress in dairy animals-management induced stress in dairy cattle:effects on reproduction[M]//FUQUAY J W.Encyclopedia of Dairy Sciences.2nd ed.Amsterdam:Academic Press, 2011:575-581.
[22] 陈浩, 王纯洁, 斯木吉德, 等.慢性热应激对放牧肉牛血液生化指标、抗氧化能力及免疫功能的影响[J].中国农业大学学报, 2021, 26(2):61-69.
CHEN H, WANG C J, SIMUJIDE, et al.Effects of chronic heat stress on blood biochemical index, immune function and antioxidant capacity of grazing beef cattle[J].Journal of China Agricultural University, 2021, 26(2):61-69.(in Chinese)
[23] 陈浩, 敖日格乐, 王纯洁, 等.慢性冷热应激对蒙古牛血清内分泌激素及抗氧化指标的影响[J].黑龙江畜牧兽医, 2018(23):87-89.
CHEN H, AORIGELE, WANG C J, et al.Effects of chronic cold and heat stress on serum endocrine hormones and antioxidant indicators in Mongolian cattle[J].Heilongjiang Animal Science and Veterinary Medicine, 2018(23):87-89.(in Chinese)
[24] 罗宗刚, 王玲, 蔡明成, 等.热应激对不同杂交组合肉牛生理指标和血液生化指标的影响[J].中国畜牧杂志, 2015, 51(11):82-85.
LUO Z G, WANG L, CAI M C, et al.Effects of heat stresson physiological index and blood biochemical index of different hybrid beef cattle[J].Chinese Journal of Animal Science, 2015, 51(11):82-85.(in Chinese)
[25] JOHNSON H D, VANJONACK W J.Effects of environmental and other stressors on blood hormone patterns in lactating animals[J].J Dairy Sci, 1976, 59(9):1603-1617.
[26] WOJTUNIK-KULESZA K A, ONISZCZUK A, ONISZCZUK T, et al.The influence of common free radicals and antioxidants on development of Alzheimer^,s Disease[J].Biomed Pharmacother, 2016, 78:39-49.
[27] 宋小珍, 付戴波, 瞿明仁, 等.热应激对肉牛血清内分泌激素含量、抗氧化酶活性及生理生化指标的影响[J].动物营养学报, 2012, 24(12):2485-2490.
SONG X Z, FU D B, QU M R, et al.Effects of heat stress on endocrine hormone content, antioxidant enzyme activity and physiological and serum biochemical indices of beef cattle[J].Chinese Journal of Animal Nutrition, 2012, 24(12):2485-2490.(in Chinese)
[28] 陈志宏, 齐聪儒, 杨松鹤, 等.器官衰老机理和自由基学说[J].承德医学院学报, 2003, 20(2):143-145.
CHEN Z H, QI C R, YANG S H, et al.Organ aging mechanism and free radical theory[J].Journal of Chengde Medical College, 2003, 20(2):143-145.(in Chinese)
[29] 陈雯雯, 李津, 郑威, 等.高温高湿对奶水牛生理和抗氧化指标的影响[J].广西畜牧兽医, 2012, 28(4):226-228.
CHEN W W, LI J, ZHENG W, et al.Effects of high temperature and humidity on physiological and antioxidant indicators of milk buffalo[J].Guangxi Journal of Animal Husbandry & Veterinary Medicine, 2012, 28(4):226-228.(in Chinese)
[30] AL-QUDAH K M.Oxidative stress resulting from subclinical nitrite poisoning in cattle[J].Toxicol Environ Chem, 2010, 92(2):347-354.
[31] SCIBILIA L S, MULLER L D, KENSINGER R S, et al.Effect of environmental temperature and dietary fat on growth and physiological responses of newborn calves[J].J Dairy Sci, 1987, 70(7):1426-1433.
[32] 孟祥坤, 曹兵海, 庄宏, 等.慢性冷应激对西门塔尔杂交犊牛免疫相关指标的影响[J].中国农业大学学报, 2010, 15(6):65-70.
MENG X K, CAO B H, ZHUANG H, et al.Effects of chronic cold stress on immune of Simmental crossbred calves[J].Journal of China Agricultural University, 2010, 15(6):65-70.(in Chinese)
[33] 郑海英, 吴佳琦, 包雨鑫, 等.热应激对科尔沁牛与科尔沁肉牛抗氧化功能的影响[J].中国牛业科学, 2021, 47(6):16-19.
ZHENG H Y, WU J Q, BAO Y X, et al.Effect of heat stress on anti-oxidative capability in Kerqin cattle and Kerqin beef cattle[J].China Cattle Science, 2021, 47(6):16-19.(in Chinese)
[34] 陈浩, 敖日格乐, 王纯洁, 等.热应激状态下不同品种肉牛免疫功能和抗氧化功能的比较研究[J].中国农业大学学报, 2019, 24(8):72-77.
CHEN H, AORIGELE, WANG C J, et al.Comparative study on the immune and antioxidant functions of different beef cattle varieties under heat stress[J].Journal of China Agricultural University, 2019, 24(8):72-77.(in Chinese)
[35] 格日乐其木格.热应激对放牧肉牛免疫功能和抗氧化能力的影响[J].当代畜禽养殖业, 2021(5):17-20.
GERILE Q M G.The effect of heat stress on immune function and antioxidant capacity of grazed beef cattle[J].Modern Livestock and Poultry Breeding Industry, 2021(5):17-20.(in Chinese)
[36] 黄德均, 向白菊, 高立芳, 等.热应激对红安格斯牛、抗旱王牛及红抗杂交牛生理指标和血液生化指标的影响[J].黑龙江畜牧兽医, 2019(16):35-39.
HUANG D J, XIANG B J, GAO L F, et al.The effect of heat stress on physiological and blood biochemical indexes of Red Angus cattle, Droughtmaster cattle and Red Angus×Droughtmaster hybrid cattle[J].Heilongjiang Animal Science and Veterinary Medicine, 2019(16):35-39.(in Chinese)
[37] TEJASWI V, BALAMURUGAN B, SAMAD H A, et al.Short communication:Differential endocrine and antioxidant responses to heat stress among native and crossbred cattle[J].J Vet Behav, 2020, 39:1-5.
[38] PAUL A, DANGI S S, GUPTA M, et al.Expression of TLR genes in Black Bengal goat (Capra hircus) during different seasons[J].Small Ruminant Res, 2015, 124:17-23.
[39] JU X H, YONG Y H, XU H J, et al.Selection of reference genes for gene expression studies in PBMC from Bama miniature pig under heat stress[J].Vet Immunol Immunopathol, 2011, 144(1-2):160-166.
[40] 赵亚星.反刍动物冷应激研究进展[J].动物营养学报, 2020, 32(11):5006-5012.
ZHAO Y X.Research progress of cold stress in ruminants[J].Chinese Journal of Animal Nutrition, 2020, 32(11):5006-5012.(in Chinese)
[41] EBRAHIMI M, TOWHIDI A.The mechanism of selenium effect on cow and suckling calves for increasing sustainability against cold stress in cold climates[C]//5th National Congress in Holstein Association of Iran.Iran, 2016:5.
[42] BHARATI J, DANGI S S, MISHRA S R, et al.Expression analysis of Toll like receptors and interleukins in Tharparkar cattle during acclimation to heat stress exposure[J].J Therm Biol, 2017, 65:48-56.
[43] PELI A, SCAGLIARINI L, BERGAMINI P F, et al.Influence of heat stress on the immunity in growing beef cattle[J].Large Anim Rev, 2013, 19(5):215-218.
[44] RODRÍGUEZ-ITURBE B, JOHNSON R J.Heat shock proteins and cardiovascular disease[J].Physio Int, 2018, 105(1):19-37.
[45] KHAN R I N, SAHU A R, MALLA W A, et al.Systems biology under heat stress in Indian cattle[J].Gene, 2021, 805:145908.
[46] RAZA S H A, HASSANIN A A, DHSHAN A I M, et al.In silico genomic and proteomic analyses of three heat shock proteins (HSP70, HSP90-α, and HSP90-β) in even-toed ungulates[J].Electron J Biotechnol, 2021, 53:61-70.
[47] GONG W J, GOLIC K G.Loss of Hsp70 in Drosophila is pleiotropic, with effects on thermotolerance, recovery from heat shock and neurodegeneration[J].Genetics, 2006, 172(1):275-286.
[48] 姜冬梅, 李士泽, 姜书磊, 等.冷应激动物相关基因表达的研究进展[J].环境与健康杂志, 2006, 23(5):471-473.
JIANG D M, LI S Z, JIANG S L, et al.Progress in study of correlative genes expression of cold stress animals[J].Journal of Environment and Health, 2006, 23(5):471-473.(in Chinese)
[49] 康玲, 胡丽蓉, 李玮, 等.冷热应激对牛PBMC和MDBK细胞活力及HSP70表达的影响[J].中国兽医学报, 2018, 38(7):1359-1365.
KANG L, HU L R, LI W, et al.Effect of cold and heat stress on cell vitality and HSP70 expression in PBMC and MDBK of cattle[J].Chinese Journal of Veterinary Science, 2018, 38(7):1359-1365.(in Chinese)
[50] MORIMOTO R I.Regulation of the heat shock transcriptional response:cross talk between a family of heat shock factors, molecular chaperones, and negative regulators[J].Genes Dev, 1998, 12(24):3788-3796.
[51] WU T C, TANGUAY R M, WU Y, et al.Presence of antibodies to heat stress proteins and its possible significance in workers exposed to high temperature and carbon monoxide[J].Biomed Environ Sci, 1996, 9(4):370-379.
[52] HOOPER H B, TITTO C G, GONELLA-DIAZA A M, et al.Heat loss efficiency and HSPs gene expression of Nellore cows in tropical climate conditions[J].Int J Biometeorol, 2019, 63(11):1475-1486.
[53] ONASANYA G O, MSALYA G M, THIRUVENKADAN A K, et al.Single nucleotide polymorphisms at heat shock protein 90 gene and their association with thermo-tolerance potential in selected indigenous Nigerian cattle[J].Trop Anim Health Prod, 2020, 52(4):1961-1970.
[54] DE FÁTIMA BRETANHA ROCHA R, BAENA M M, DE CÁSSIA ESTOPA A, et al.Differential expression of HSF1 and HSPA6 genes and physiological responses in Angus and Simmental cattle breeds[J].J Therm Biol, 2019, 84:92-98.
[55] COLLIER R J, COLLIER J L, RHOADS R P, et al.Invited review:genes involved in the bovine heat stress response[J].J Dairy Sci, 2008, 91(2):445-454.
[56] 白丹丹, 敖日格乐, 王纯洁, 等.慢性冷热应激对三河牛血液生化指标及相关基因表达的影响[J].中国农业大学学报, 2017, 22(8):50-56.
BAI D D, AORIGELE, WANG C J, et al.Influences of chronic cold and heat stress on blood biochemical parameters and related gene expression in Sanhe cattle[J].Journal of China Agricultural University, 2017, 22(8):50-56.(in Chinese)
[57] 李玮.温度应激对牛HSP70表达和相关生理生化指标的影响[D].银川:宁夏大学, 2015.
LI W.Effects of thermo stress on the expressioo of HSP70 and the relatively physical and biochemical indexes[D].Yinchuan:Ningxia University, 2015.(in Chinese)
[58] HU L R, BRITO L F, ABBAS Z, et al.Investigating the short-term effects of cold stress on metabolite responses and metabolic pathways in inner-mongolia Sanhe cattle[J].Animals, 2021, 11(9):2493.
[59] KUMAR A, ASHRAF S, GOUD T S, et al.Expression profiling of major heat shock protein genes during different seasons in cattle (Bos indicus) and buffalo (Bubalus bubalis) under tropical climatic condition[J].J Therm Biol, 2015, 51:55-64.
[60] BEHL R, BEHL J, SADANA D K, et al.Characterization of hsp70 gene promoter for cis-acting elements in Indian zebu cattle of Hariana breed[J].Anim Biotechnol, 2014, 25(3):160-164.
[61] LACETERA N, BERNABUCCI U, SCALIA D, et al.Heat stress elicits different responses in peripheral blood mononuclear cells from brown Swiss and Holstein cows[J].J Dairy Sci, 2006, 89(12):4606-4612.
[62] HU L R, MA Y, LIU L L, et al.Detection of functional polymorphisms in the hsp70 gene and association with cold stress response in Inner-Mongolia Sanhe cattle[J].Cell Stress Chaperones, 2019, 24(2):409-418.
[63] BHAT S, KUMAR P, KASHYAP N, et al.Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle[J].Vet World, 2016, 9(2):113-117.
[64] 甘佳, 付茂忠, 王巍, 等.蜀宣花牛与荷斯坦牛、娟荷杂交牛HSP70基因多态性及表达量分析[J].黑龙江畜牧兽医, 2018(24):50-53, 260.
GAN J, FU M Z, WANG W, et al.Analysis of polymorphism and expression level of HSP70 gene in Shuxuan, Holstein, and Jersey×Holstein crossbred cattle[J].Heilongjiang Animal Science and Veterinary Medicine, 2018(24):50-53, 260.(in Chinese)
[65] SUHENDRO I, JAKARIA J, NOOR R R.The genetic diversity of heat shock protein 70 gene at promoter and 5'untranslated region in beef cattle[J].J Indones Trop Anim Agric, 2021, 46(2):136-144.
[66] VIJAYAKUMAR P, SINGARAVADIVELAN A, MISHRA A, et al.Whole-Genome comparative analysis reveals genetic mechanisms of disease resistance and heat tolerance of tropical Bos indicus cattle breeds[J].Genome, 2022, 65(4):241-254.
[67] GAUGHAN J B, MADER T L, HOLT S M.Assessing the heat tolerance of 17 beef cattle genotypes[J].Int J Biometeorol, 2010, 54(6):617-627.
[68] 甘佳, 王巍, 唐慧, 等.热应激对牛的影响及与HSP70基因关联性研究进展[J].草业与畜牧, 2016(2):56-58.
GAN J, WANG W, TANG H, et al.Research progress on effect of heat stress on cattle and its relationship with HSP70 gene polymorphism[J].Journal of Grassland and Forage Science, 2016(2):56-58.(in Chinese)
[69] DANGI S S, BHARATI J, SAMAD H A, et al.Expression dynamics of heat shock proteins (HSP) in livestock under thermal stress[M]//ASEA A A A, KAUR P.Heat Shock Proteins in Veterinary Medicine and Sciences.Cham:Springer, 2017:37-79.
[70] HARIYONO D N H, PRIHANDINI P W.Association of selected gene polymorphisms with thermotolerance traits in cattle-A review[J].Anim Biosci, 2022, 35(11):1635-1648.
[71] RONG Y, ZENG M F, GUAN X W, et al.Association of HSF1 genetic variation with heat tolerance in Chinese cattle[J].Animals, 2019, 9(12):1027.
[72] SENGAR G S, DEB R, SINGH U, et al.Differential expression of microRNAs associated with thermal stress in Frieswal (Bos taurus x Bos indicus) crossbred dairy cattle[J].Cell Stress Chaperones, 2018, 23(1):155-170.
[73] TIAN R G, NANAIE H A, WANG X, et al.Genomic adaptation to extreme climate conditions in beef cattle as a consequence of cross-breeding program[J].BMC Genomics, 2023, 24(1):186.
[74] JIA P, CAI C C, QU K X, et al.Four novel SNPs of MYO1A gene associated with heat-tolerance in Chinese cattle[J].Animals, 2019, 9(11):964.
[75] LIANG L, MA G F, CHEN K, et al.EIF2AK4 mutation in pulmonary veno-occlusive disease:A case report and review of the literature[J].Medicine (Baltimore), 2016, 95(39):e5030.
[76] LIU Y, LÁSZLÓ C, LIU Y, et al.Regulation of G₁ arrest and apoptosis in hypoxia by PERK and GCN2-mediated eIF2α phosphorylation[J].Neoplasia, 2010, 12(1):61-68.
[77] EDEA Z, DADI H, DESSIE T, et al.Genome-wide scan reveals divergent selection among taurine and zebu cattle populations from different regions[J].Anim Genet, 2018, 49(6):550-563.
[78] BOHLOULI M, HALLI K, YIN T, et al.Genome-wide associations for heat stress response suggest potential candidate genes underlying milk fatty acid composition in dairy cattle[J].J Dairy Sci, 2022, 105(4):3323-3340.
[79] ZENG L L, QU K X, ZHANG J C, et al.Genes related to heat tolerance in cattle-a review[J].Anim Biotechnol, 2023, 34(5):1840-1848.
[80] SAJJANAR B, DEB R, SINGH U, et al.Identification of SNP in HSP90AB1 and its association with the relative thermotolerance and milk production traits in Indian dairy cattle[J].Anim Biotechnol, 2015, 26(1):45-50.
[81] 李进春, 雷正达, 龚洋, 等.五粮型白酒糟日粮添加不同饲料添加剂对肉牛生长和血清免疫指标的影响[J].黑龙江畜牧兽医, 2020(4):91-94, 98.
LI J C, LEI Z D, GONG Y, et al.Effects of different feed additives in five grain Baijiu grains diet on growth and serum immune indexes of beef cattle[J].Heilongjiang Animal Science and Veterinary Medicine, 2020(4):91-94, 98.(in Chinese)
[82] 何振富, 董俊, 郝怀志, 等.不同饲料添加剂对舍饲肉牛血清生化指标、免疫功能及生长激素的影响[J].中国饲料, 2016(18):24-28.
HE Z F, DONG J, HAO H Z, et al.Efects of the different feed additives on serum biochemical indicator, immune function and growth hormone in the barn feeding cattle[J].China Feed, 2016(18):24-28.(in Chinese)
[83] 隋朝阳.不同季节肉牛饲养的管理要点[J].现代畜牧科技, 2016(2):7.
SUI Z Y.Key management points for beef cattle breeding in different seasons[J].Modern Animal Husbandry Science & Technology, 2016(2):7.(in Chinese)
[84] DAURIA B D, SIGDEL A, PETRINI J, et al.Genetic effects of heat stress on milk fatty acids in Brazilian Holstein cattle[J].J Dairy Sci, 2022, 105(4):3296-3305.
[85] MORALES R M, MENÉNDEZ-BUXADERA A, DEMYDA-PEYRÁS S, et al.Genetic effects of season on the preweaning growth of beef cattle:A first approach to Retinta calves[J].Rev Colomb Cienc Pec, 2019, 33(2):134-143.
[86] FATHONI A, BOONKUM W, CHANKITISAKUL V, et al.An appropriate genetic approach for improving reproductive traits in crossbred Thai-Holstein cattle under heat stress conditions[J].Vet Sci, 2022, 9(4):163.
[87] RAHIMI J, MUTUA J Y, NOTENBAERT A M O, et al.Heat stress will detrimentally impact future livestock production in East Africa[J].Nat Food, 2021, 2(2):88-96.
[88] STRANDÉN I, KANTANEN J, LIDAUER M H, et al.Animal board invited review:Genomic-based improvement of cattle in response to climate change[J].Animal, 2022, 16(12):100673.