猫疱疹病毒1型的检测及一株分离毒株的致病性

doi:10.11843/j.issn.0366-6964.2024.05.042

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (5): 2253-2258.doi: 10.11843/j.issn.0366-6964.2024.05.042

猫疱疹病毒1型的检测及一株分离毒株的致病性

邓梏男¹, 张家祺¹, 保志鹏⁴, 陈涛云¹, 喻琦胜¹, 丁露¹, 朱晨曦¹, 王怡¹, 任玉鹏^1,2, 贺超^3*, 张斌^1,2*

1. 西南民族大学畜牧兽医学院, 成都 610041;
2. 青藏高原动物遗传资源保护与利用教育部/四川省重点实验室, 成都 610041;
3. 四川省兽药监察所, 成都 610041;
4. 云南农业职业技术学院, 昆明 650212

收稿日期:2023-06-15 出版日期:2024-05-23 发布日期:2024-05-27
通讯作者: 张斌，主要从事动物病原分子生物学研究，E-mail：binovy@sina.com；贺超，主要从事动物疫病防控研究及兽药监察工作，E-mail:525069528@qq.com
作者简介:邓梏男(1996-)，男，四川宜宾人，硕士生，主要从事动物疫病防控研究，E-mail：1181389642@qq.com
基金资助:
国家农业产业技术体系四川兽药创新团队(SCCXTD-2020-18)；西南民族大学人才支持基金项目(RQD2021004)

Detection of Feline Herpesvirus Type 1 and Pathogenicity of an Isolated Strain

DENG Gunan¹, ZHANG Jiaqi¹, BAO Zhipeng⁴, CHEN Taoyun¹, YU Qisheng¹, DING Lu¹, ZHU Chenxi¹, WANG Yi¹, REN Yupeng^1,2, HE Chao^3*, ZHANG Bin^1,2*

1. College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China;
2. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education/Sichuan Provine, Chengdu 610041, China;
3. Sichuan Provincial Veterinary Drug Supervision Institute, Chengdu 610041, China;
4. Yunnan Vocational and Technical College of Agriculture, Kunming 650212, China

Received:2023-06-15 Online:2024-05-23 Published:2024-05-27

摘要/Abstract

摘要： 旨在调查成都地区的猫疱疹病毒1型(feline herpesvirus-1, FHV-1)的流行及遗传变异情况。试验选取2020—2023年从成都地区采集的178份具有呼吸道症状猫的眼、鼻及口咽拭子进行FHV-1核酸检测、gE基因遗传进化分析、FHV-1的分离鉴定和致病性研究。结果发现，成都地区FHV-1阳性率为11.8%(21/178)；其中宠物医院FHV-1阳性率为6.4%(7/110)，猫舍FHV-1阳性率为20.6%(14/68)，表明在猫舍中FHV-1感染率更高。FHV-1 gE基因的遗传进化分析结果表明，本研究扩增出的5株gE基因和疫苗株的核苷酸同源性在99.1%~100%，并发现PX-9株存在3个独特的氨基酸突变位点(L259Q、C294I、W295F)。通过CRFK细胞对5份阳性样本进行病毒的分离培养，结果PX-9毒株被成功分离鉴定。对PX-9毒株进行动物回归试验，结果表明攻毒组猫只表现出眼鼻分泌物增多，打喷嚏等临床症状，在接毒后第4天病毒拷贝数最高，为9.05×10⁷ copies·μL^-1，且排毒期较长；攻毒猫只FHV-1病毒载量检测结果显示，气管及肺组织病毒拷贝数分别为1.7×10⁷和3.5×10⁴ copies·μg^-1，其它组织核酸阴性。综上表明，在成都地区仍然存在FHV-1流行，并且流行毒株有较强的致病性。

关键词: FHV-1, 流行情况, 遗传变异, 动物回归试验

Abstract: The aim of this study was to investigate the prevalence and genetic diversity of feline herpesvirus-1 (FHV-1) in Chengdu city. From 2020 to 2023, a total of 178 conjunctiva, pharynx and nose swabs were collected from cats with respiratory symptoms in Chengdu city. The collected samples were subjected to FHV-1 PCR detection, gE gene genetic evolutionary analysis, viral isolation and identification, as well as pathogenicity. The results showed that, the FHV-1 positivity rate in Chengdu city was 11.8% (21/178). In which the FHV-1 positivity rate of veterinary hospitals was 6.4% (7/110) and 20.6% (14/68) in catteries, suggested that the higher rate of FHV-1 infection was in catteries. The genetic evolutionary analysis of five FHV-1 gE genes showed that the nucleotide homology was 99.1%~100% to the vaccine strains,and three unique amino acid mutation sites were found in PX-9 strain (L259Q, C294I, W295F). Five positive samples were isolated and cultured by CRFK cell, only PX-9 strain was successfully isolated and identified from CRFK cells. The results of animal regression experiment by PX-9 strain showed that all cats in the infected group exhibited clinical symptoms, such as increased ocular, nasal secretions and sneezing. The highest viral load was found on the 4th day after inoculation (9.05×10⁷copies·μL^-1), and the detoxification period is relatively long. The results of FHV-1 viral load were 1.7×10⁷ and 3.5×10⁴copies·μg^-1 in trachea and lungs of infected group, respectively. The other tissues were negative for FHV-1 DNA. The results of this study indicated that FHV-1 was still prevalent in Chengdu and the prevalent strains had highly pathogenicity.

Key words: FHV-1, prevalence, genetic variation, animal regression experiment

中图分类号:

邓梏男, 张家祺, 保志鹏, 陈涛云, 喻琦胜, 丁露, 朱晨曦, 王怡, 任玉鹏, 贺超, 张斌. 猫疱疹病毒1型的检测及一株分离毒株的致病性[J]. 畜牧兽医学报, 2024, 55(5): 2253-2258.

DENG Gunan, ZHANG Jiaqi, BAO Zhipeng, CHEN Taoyun, YU Qisheng, DING Lu, ZHU Chenxi, WANG Yi, REN Yupeng, HE Chao, ZHANG Bin. Detection of Feline Herpesvirus Type 1 and Pathogenicity of an Isolated Strain[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2253-2258.

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猫疱疹病毒1型的检测及一株分离毒株的致病性

Detection of Feline Herpesvirus Type 1 and Pathogenicity of an Isolated Strain

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