双重放大酶免疫传感器检测鸡白痢鸡伤寒沙门菌

Abstract

Abstract:

The aim of this work is to seek a new rapid detection technology for Salmonella pullorum and Salmonella gallinarum. In this report, we firstly combined multiwalled carbon nanotubes with ionic liquid ［BMIM］PF₆ to modify four-channel screen-printed carbon electrode to propose an enzyme immunosensor with double amplified response signal for detection of Salmonella pullorum and Salmonella gallinarum. The surface morphology of the modified electrode was characterized by atomic force microscopy, while the electrochemical properties were determined by cyclic voltammetry. The results showed that under the optimal assay conditions, a good linear response occurred in the concentration range of 10³-10⁹ cfu·mL^－1 of Salmonella pullorum and Salmonella gallinarum, with a low detection limit of 3.93×10² cfu·mL^－1 (S/N=3). The stability test of the fabricated immunosensor showed that it remained 90.15% of its original response signal after storage of 28 days under 4 ℃. The performance evaluation of the proposed immunosensor showed that it had high stability, good specificity, acceptable reproducibility and accuracy. The results demonstrated that the proposed enzyme immunosensor with combination of multi-walled carbon nanotubes and ionic liquid achieved the effect of double amplification of detection signal which enabled it highly sensitive in detection. Ionic liquid was also helpful for maintaining bioactivity of enzyme labeled antibody, thereby prolonging effective time of the immune electrode. The fabrication of the enzyme immunosensor pursuant to this method provides a good reference model for construction of enzyme immunosensor for rapid detection of other pathogenic bacterium.

CLC Number:

HU Chun-mei, DOU Wen-chao, ZHAO Guang-ying. Enzyme Immunosensor of Double Amplification for Detection of Salmonella pullorum and Salmonella gallinarum[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Enzyme Immunosensor of Double Amplification for Detection of Salmonella pullorum and Salmonella gallinarum

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